Team:LCG-UNAM-Mexico/Parts

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Revision as of 06:19, 28 October 2008

LCG-UNAM-Mexico:Parts

Header image
iGEM 2008 TEAM
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Parts
Constructed Parts
The following are the bioparts that we are bringing into play for our project:
   
Regulatory Parts
BioBrick Number:

BBa_K119000
Part Name: Constitutive weak promoter of lacZ
Description: LacZ constitutive promoter, without including operators.
Length: 38 bp
Designed by: Miguel Angel Ramirez-Romero
References:  
   
BioBrick Number:

BBa_K119001
Part Name: Mutated LacZ promoter
Description: Constitutive weak promoter of lacZ which sequence was changed at position 29 (T->G) in order to make it weaker
 
Length: 38 bp
Designed by: Miguel Angel Ramirez-Romero
References:  
   
BioBrick Number:

BBa_K119002
Part Name: RcnR operator (represses RcnA)
Description: Operator for RcnR(YohL) the negative regulator of niquel extrusion pump(RcnA) in E.coli
Length: 83 bp
Designed by: Libertad Pantoja
References:  
   
Coding Parts
BioBrick Number:

BBa_K119003
Part Name: RcnA (YohM)
Description: Nickel efflux pump from E.coli
Length: 833 bp
Designed by: Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo
 
References:  
   
Primers
BioBrick Number:

BBa_K119004
Part Name: Upper primer for aiiA with normal lacZ promoter
Description:

Upper primer used to add lacZ promoter to aiiA in the device LuxR_cI_aiiA_Normal lacZ promoter

 
Length: 67 bp
Designed by: Martin del Castillo V.
References:  
   
BioBrick Number:

BBa_K119005
Part Name: Upper primer for aiiA with mutated lacZ promoter
Description:

Upper primer used to add a mutated version of lacZ promoter to aiiA in the device LuxR_cI_aiiA_Normal lacZ promoter

 
Length: 67 bp
Designed by: Martin del Castillo V.
References:  
   
BioBrick Number:

BBa_K119007
Part Name: Upper primer for RcnA
Description:

Upper primer used to add cI promoter upstream of the RcnR operator in the device cI+AVGreg_RcnRop_RcnA()

 
Length: 73 bp
Designed by: Libertad Pantoja
References:  
   
BioBrick Number:

BBa_K119008
Part Name: Lower primer for RcnA (BBa_K119003)
Description:

Lower primer for RcnA (BBa_K119003). Includes just the end of the RcnA coding region.
Length: 21 bp
Designed by: Libertad Pantoja
References:  
   
Devices  
BioBrick Number:

BBa_K119009
Part Name: cI promoter RcnR op RcnA
Description:

This device encodes the extrusion pump and uses two repressible promoters in order to stop the niquel extrusion.

 
Length: 969 bp
Designed by: Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo, Martín del Castillo
 
References:  
   
BioBrick Number:

BBa_K119010
Part Name: LuxR_cI_aiiA_Normal lacZ promoter
Description:

This device encodes cI, the protein which controls RcnA. It also contains LuxR, which Induces the expression of cI and aiiA

 
Length: 2873 bp
Designed by: Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo
References:  
   
BioBrick Number:

BBa_K119011
Part Name: LuxR_cI_aiiA_Mutated lacZ promoter
Description:

This device encodes cI, the protein which controls RcnA. It also contains LuxR, which Induces the expression of cI and aiiA

 
Length: 2873 bp
Designed by: Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja, Martin del Castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, Enrique Paz Cortes, Atahualpa Castillo, Isaac Rodriguez, Minerva Trejo
References:  
   
     

 
 
             

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