Team:Princeton/Experiments
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===Bio-brick Creation=== | ===Bio-brick Creation=== | ||
'''Lab Protocols''' | '''Lab Protocols''' | ||
- | + | 1. Primer Design | |
+ | 2. PCR | ||
+ | 3. Run Gel | ||
+ | 4. Gel Extraction | ||
+ | 5. Digestion | ||
+ | 6. CIP Treatment | ||
+ | 7. PCR Purification | ||
+ | 8. Ligation | ||
+ | 9. Transformation and Plating | ||
+ | 10. Extract DNA (Miniprep) | ||
+ | 11. Restriction Map/ Digest | ||
+ | 12. Re-transform with selected plasmid | ||
+ | 13. Extract DNA (Maxiprep or Midiprep) | ||
+ | 14. Sequence | ||
- | + | Cell Culture |
Revision as of 08:23, 28 October 2008
PRINCETON IGEM 2008
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Bio-brick Creation
Lab Protocols 1. Primer Design 2. PCR 3. Run Gel 4. Gel Extraction 5. Digestion 6. CIP Treatment 7. PCR Purification 8. Ligation 9. Transformation and Plating 10. Extract DNA (Miniprep) 11. Restriction Map/ Digest 12. Re-transform with selected plasmid 13. Extract DNA (Maxiprep or Midiprep) 14. Sequence
Cell Culture