Team:Princeton/Experiments

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PRINCETON IGEM 2008

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Bio-brick Creation

Lab Protocols

1. Primer Design

2. PCR Amplification

3. Run Gel/ Gel extraction

4. PCR SOEing

5. Digestion

6. CIP Treatment

7. PCR Purification

8. Ligate DNA

9. Transformation and Plating

10. Extract DNA (Miniprep)

11. Restriction Map/ Restriction Digest

12. Re-transform with selected plasmid

13. Extract DNA (Maxiprep or Midiprep)

14. Sequence


Bio-Brick Verification

Cell Culture Process

1. Lentivirus Production

2. Lentivirus Harvesting

3. Infection


Also,

1. Transfection (nonviral)

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