Team:Princeton/Experiments

From 2008.igem.org

(Difference between revisions)
(Bio-brick Creation)
(Bio-brick Creation)
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===Bio-brick Creation===
===Bio-brick Creation===
'''Lab Protocols'''
'''Lab Protocols'''
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1. Primer Design
1. Primer Design
2. PCR
2. PCR
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13. Extract DNA (Maxiprep or Midiprep)
13. Extract DNA (Maxiprep or Midiprep)
14. Sequence
14. Sequence
 +
Cell Culture
Cell Culture

Revision as of 08:25, 28 October 2008

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PRINCETON IGEM 2008

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Bio-brick Creation

Lab Protocols

1. Primer Design 2. PCR 3. Run Gel 4. Gel Extraction 5. Digestion 6. CIP Treatment 7. PCR Purification 8. Ligation 9. Transformation and Plating 10. Extract DNA (Miniprep) 11. Restriction Map/ Digest 12. Re-transform with selected plasmid 13. Extract DNA (Maxiprep or Midiprep) 14. Sequence


Cell Culture