Team:Warsaw/Calendar-Main/6 June 2008

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<h3> Blue/white and rifampicin test </h3>
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<h3> Blue/white and rifampicin test #1</h3>
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<h4>Michał L., Ewa, Marcin:</h4>
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<h4>Michał L., Ewa, Marcin</h4>
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<ol><li>Making <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a>. </li></ol><br>
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<p>Making <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> carrying <a href="https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320">pZC320</a>. </p><br>
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<html><h3>Preparation of pMPMT5-AID+AIDT7 construct</h3>
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<h4>Michał K.:</h4><ol>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5-AID+AIDT7.</li>
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<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPM-T5-AID+AIDT7 with BamHI.</li>
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<li>DNA gel electrophoresis.</li></ol>
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<html><h3>Preparation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID%2BAID-T7>pMPMT5-AID+AIDT7</a> construct</h3>
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<h4>Michał K.</h4><ol>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID%2BAID-T7>pMPM-T5-AID+AIDT7</a>.</li>
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<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID%2BAID-T7>pMPM-T5-AID+AIDT7</a> with BamHI (BamHI buffer).</li>
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<li>DNA gel electrophoresis. <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/6_June_2008#fig1">Fig. 1</a></li></ol> <br>
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<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/3/34/Trawienie_aat_kontrola_04_06_2008.jpg" width=250></a>
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<var><b>Fig. 1.</b> Control digest of the pMPMT5-AID+AIDT7 construct.<br> Lane 2 - 5: isolated plasmids digested with BamHI</var>
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Latest revision as of 16:52, 28 October 2008

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Blue/white and rifampicin test #1

Michał L., Ewa, Marcin

Making chemocompetent E. coli TOP10 carrying pZC320.


Preparation of pMPMT5-AID+AIDT7 construct

Michał K.

  1. Isolation of hypothetical pMPM-T5-AID+AIDT7.
  2. Control Digest of pMPM-T5-AID+AIDT7 with BamHI (BamHI buffer).
  3. DNA gel electrophoresis. Fig. 1

Fig. 1. Control digest of the pMPMT5-AID+AIDT7 construct.
Lane 2 - 5: isolated plasmids digested with BamHI

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