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Team:Chiba/parts/BBa K084007
From 2008.igem.org
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====Senders:XL10Gold, Receiver:JW1908, 30°C==== | ====Senders:XL10Gold, Receiver:JW1908, 30°C==== | ||
[[Image:Chiba_talks_XL10G_30-1_RS1_01.gif|frame|left|Fig. <br>E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.]] | [[Image:Chiba_talks_XL10G_30-1_RS1_01.gif|frame|left|Fig. <br>E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.]] | ||
| + | [[Image:Chiba_talks_XL10G_30-1_RS1_02.gif|frame|left|Fig. <br>E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.]] | ||
====others==== | ====others==== | ||
Revision as of 17:21, 28 October 2008
| Home | The Team | The Project | Parts Submitted to the Registry | Reference | Notebook | Acknowledgements |
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parts usage
[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0178 LasI(no LVA)])
日本語:IPTGにより、シュードモナス由来の3OC12HSL合成酵素,LasIを発現するデバイス。
English:
Experiment
To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.
- Transformed Senders into E.coli strains(XL10GOLD or JW1908) and Receiver into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 30°C.
- Measured intensity of green fluorescence at regular time intervals.
- crosstalk experients at various conditions.
- E.Coli strain JW1908 or XL10Gold.
- Reaction Temparature 37 or 30.
- Cell number.
Results
Sender:JW1908, Receiver:JW1908, 37°C
Fig.
E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.
E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Sender:JW1908, Receiver:JW1908, 30°C
Senders:XL10Gold, Receiver:JW1908, 37°C
Fig.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Senders:XL10Gold, Receiver:JW1908, 30°C
Fig.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Fig.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
others
Fig.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Fig.
E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Fig.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30,Receiver cells/Sender cells = 1.
