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Team:Hawaii/Protocols
From 2008.igem.org
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* [[Team:Hawaii/Protocols/Transformation_E_coli|Transformation of '' E. coli'']] | * [[Team:Hawaii/Protocols/Transformation_E_coli|Transformation of '' E. coli'']] | ||
* [[Team:Hawaii/Protocols/Transformation_PCC6803|Triparental conjugation of ''Synechocystis'' sp. PCC6803]] | * [[Team:Hawaii/Protocols/Transformation_PCC6803|Triparental conjugation of ''Synechocystis'' sp. PCC6803]] | ||
| - | * Alkaline lysis / plasmid | + | * Alkaline lysis / plasmid isolation |
| + | * Solid growth media | ||
| + | :* LB | ||
| + | :* LB + amp<sub>100</sub> | ||
| + | :* LB + kan<sub>20</sub> + amp <sub>50</sub> | ||
| + | :* LB + sp<sub>100</sub> | ||
| + | :* LB + sp<sub>100</sub> + sm<sub>50</sub> | ||
| + | :* BG-11 + 5% LB | ||
| + | :* BG-11 | ||
| + | :* BG-11 + sp<sub>2.5</sub> + sm<sub>2.5</sub> | ||
| + | * Liquid growth media | ||
{{Team:Hawaii/Footer}} | {{Team:Hawaii/Footer}} | ||
__NOTOC__ | __NOTOC__ | ||
Revision as of 07:04, 24 June 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
List of Protocols
This is a list of tried and true protocols used by our team.
- Making competent cells
- Transformation of E. coli
- Triparental conjugation of Synechocystis sp. PCC6803
- Alkaline lysis / plasmid isolation
- Solid growth media
- LB
- LB + amp100
- LB + kan20 + amp 50
- LB + sp100
- LB + sp100 + sm50
- BG-11 + 5% LB
- BG-11
- BG-11 + sp2.5 + sm2.5
- Liquid growth media
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
