Imperial College/12 August 2008
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|{{#calendar: title=Imperial_College |year=2008 | month=08}} | |{{#calendar: title=Imperial_College |year=2008 | month=08}} | ||
|{{#calendar: title=Imperial_College |year=2008 | month=09}} | |{{#calendar: title=Imperial_College |year=2008 | month=09}} | ||
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= 12 August 2008 = | = 12 August 2008 = | ||
- | == | + | ==B.subtilis== |
+ | *Mini prep of 5ml ''E.coli'' cultures containing the pDR110/pDR111 | ||
+ | *Competent ''B.subtilis'' prepared | ||
- | + | ==PCR Cloning== | |
- | Designed Primers for PCR cloning of AmyE integration sequences and Spectinomycin resistance from plasmid pDR111 | + | *Designed Primers for PCR cloning of XylR gene and EpsE integration sequences from the ''B.subtilis'' chromosome |
+ | |||
+ | *Designed Primers for PCR cloning of AmyE integration sequences and Spectinomycin resistance from plasmid pDR111 | ||
+ | |||
+ | ==Dry Lab== | ||
+ | |||
+ | * wrote a code on matlab to obtain velocity, direction, run time and tumble time from the positions of the cell at each frame | ||
+ | * Did analysis on exponential distribution, plotting of Posterior of parameter lambda in response to obtained synthetic data samples generated. | ||
+ | <br> | ||
+ | {{Imperial/EndPage|Notebook|Notebook}} |
Latest revision as of 20:35, 28 October 2008
12 August 2008B.subtilis
PCR Cloning
Dry Lab
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