Imperial College/15 August 2008
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|{{#calendar: title=Imperial_College |year=2008 | month=08}} | |{{#calendar: title=Imperial_College |year=2008 | month=08}} | ||
|{{#calendar: title=Imperial_College |year=2008 | month=09}} | |{{#calendar: title=Imperial_College |year=2008 | month=09}} | ||
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- | = | + | =15 August 2008= |
- | ==Wet Lab== | + | =='''Wet Lab'''== |
+ | |||
+ | ===Cloning=== | ||
*Plates form yestreday's transformations checked. | *Plates form yestreday's transformations checked. | ||
**J31005, B0015, J04650 and I13401 transformants grew overnight but no growth was registered for the C0012 transformant nor the controls. | **J31005, B0015, J04650 and I13401 transformants grew overnight but no growth was registered for the C0012 transformant nor the controls. | ||
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*PCR primers for XylR, LacI and AmyE integration sequences designed and sent for synthesis | *PCR primers for XylR, LacI and AmyE integration sequences designed and sent for synthesis | ||
- | == | + | ===''B.subtilis''=== |
+ | *The transformation protocol 2 [http://openwetware.org/wiki/IGEM:IMPERIAL/2008/Prototype/Wetlab/Transformation_protocol_4#Transformation_Protocol_2| (click link here for the protocol)] was performed yesterday. The transformed cells have not yet grown on the LB agar plates containing 100ug/ul streptinomycin. We will leave over the weekend to grow further. | ||
+ | <br> | ||
+ | {{Imperial/EndPage|Notebook|Notebook}} |
Latest revision as of 20:36, 28 October 2008
15 August 2008Wet LabCloning
B.subtilis
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