Wisconsin: Lignin Project/26 June 2008

From 2008.igem.org

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'''Team Fungus:''' <br>
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Performed ligation of insert and vector with ratios of 0:1, 1:0, 1:3, 3:1, and 1:1 <br>
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Perform transformation of plasmid into E. coli (BL21) and plated to grow overnight
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<div style="width:800px; height:147px; border:0px; margin:0px;">[[Image:Igemwibanner.gif]]</div>
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{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
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== Team Sorbitol ==
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Found that creating fresh proteinase K solution every time will work better.
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Designed and set up a gradiant PCR (using Herculase).
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|'''Team Sorbitol:'''
 +
Found that creating fresh proteinase K solution every time will work better.<br>
 +
Designed and set up a gradiant PCR (using Herculase).<br>
 +
Several successful bands for ''srlD'' were obtained.<br>
 +
The PCR product was purified and a second pcr reaction using the product was performed to maximize our gene sample.<br>
 +
Also designed sequencing primers to test for ''srlD'' in both pBAD30 and pBAD18.<br>
 +
Found there was once a commercial kit for quantifying sorbitol (Boehringer Manheim), however it was now not available in the market.<br>
-
Several successful bands for ''srlD'' were obtained.
+
'''Team Fungus:''' <br>
-
 
+
Performed ligation of insert and vector with ratios of 0:1, 1:0, 1:3, 3:1, and 1:1 <br>
-
The PCR product was purified and a second pcr reaction using the product was performed to maximize our gene sample.
+
Perform transformation of plasmid into E. coli (BL21) and plated to grow overnight
-
 
+
|}
-
Also designed sequencing primers to test for ''srlD'' in both pBAD30 and pBAD18.
+

Latest revision as of 02:50, 29 October 2008

Igemwibanner.gif
Team Sorbitol:

Found that creating fresh proteinase K solution every time will work better.
Designed and set up a gradiant PCR (using Herculase).
Several successful bands for srlD were obtained.
The PCR product was purified and a second pcr reaction using the product was performed to maximize our gene sample.
Also designed sequencing primers to test for srlD in both pBAD30 and pBAD18.
Found there was once a commercial kit for quantifying sorbitol (Boehringer Manheim), however it was now not available in the market.

Team Fungus:
Performed ligation of insert and vector with ratios of 0:1, 1:0, 1:3, 3:1, and 1:1
Perform transformation of plasmid into E. coli (BL21) and plated to grow overnight

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