Team:Chiba/parts/BBa K084007

From 2008.igem.org

(Difference between revisions)
(→Sender:JW1908, Receiver:JW1908, 30°C)
 
(25 intermediate revisions not shown)
Line 13: Line 13:
===parts usage===
===parts usage===
-
[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0178 LasI(no LVA)])==
+
[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0178 LasI(no LVA)])
 +
 
[[Image:Tet-lasi chiba.gif]]
[[Image:Tet-lasi chiba.gif]]
Line 20: Line 21:
English:
English:
-
===Experients and Results===
+
===Experiment===
 +
To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.
 +
 +
#Transformed Senders into E.coli strains(XL10GOLD or JW1908) and Receiver into E.coli strain(JW1908).
 +
#Inoculated them independently in liquid media. Incubated at 37°C 12h
 +
#Mixed them.
 +
#Incubated at 30°C.
 +
#Measured intensity of green fluorescence at regular time intervals.
 +
*[[Team:Chiba/protocol/phenotype/timedelay|crosstalk experients]] at various conditions.
 +
#E.Coli strain JW1908 or XL10Gold.
 +
#Reaction Temparature 37 or 30.
 +
#Cell number.
 +
 
 +
===Results===
 +
====Sender:JW1908, Receiver:JW1908, 37°C====
 +
[[Image:Chiba_communication_JW1908_37_RS1_01.gif‎|frame|left|Fig.　　<br>E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.]]
 +
[[Image:Chiba_talks_JW1908_37_RS1_02.gif‎|frame|left|Fig.　　<br>E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.]]
 +
====Sender:JW1908, Receiver:JW1908, 30°C====
 +
[[Image:Chiba_talks_JW1908_30_RS1_01.gif|frame|left|Fig.　　<br>E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.]]
 +
[[Image:Chiba_talks_JW1908_30_RS1_02.gif|frame|left|Fig.　　<br>E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.]]
 +
 
 +
====Senders:XL10Gold, Receiver:JW1908, 37°C====
 +
[[Image:Chiba_communication_XL10Gol_37_RS1_01.gif‎|frame|left|Fig.　　<br>E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.]]
 +
 
 +
[[Image:Chiba_talks_XL10G_37_RS1_02.gif‎|frame|left|Fig.　　<br>E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.]]
 +
 
 +
[[Image:Chiba_talks_XL10G_37_RS1_03.gif‎|frame|left|Fig.　　<br>E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.]]
 +
====Senders:XL10Gold, Receiver:JW1908, 30°C====
 +
[[Image:Chiba_talks_XL10G_30-1_RS1_01.gif‎‎|frame|left|Fig.　　<br>E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.]]
 +
[[Image:Chiba_talks_XL10G_30-1_RS1_02.gif‎‎‎|frame|left|Fig.　　<br>E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.]]
 +
 
 +
 
 +
====others====
 +
[[Image:BBa_K084007_XL10G_30_RS_1.gif|frame|left|Fig.　　<br>E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.]]
 +
 
 +
[[Image:Chiba_BBa_K084007_XL10Gold_37_RS_1.gif‎|frame|left|Fig.　　<br>E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.]]
 +
 
 +
[[Image:Chiba_LasI_JW1908_37_RS_1_.gif‎|frame|left|Fig.　　<br>E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30,Receiver cells/Sender cells = 1.]]

Latest revision as of 03:18, 29 October 2008

Chiba-U.gif

Home The Team The Project Parts Submitted to the Registry Reference Notebook Acknowledgements


parts usage

[http://partsregistry.org/Part:BBa_K084007 BBa_K084007]([http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0178 LasI(no LVA)])

Tet-lasi chiba.gif

日本語:IPTGにより、シュードモナス由来の3OC12HSL合成酵素,LasIを発現するデバイス。

English:

Experiment

To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.

  1. Transformed Senders into E.coli strains(XL10GOLD or JW1908) and Receiver into E.coli strain(JW1908).
  2. Inoculated them independently in liquid media. Incubated at 37°C 12h
  3. Mixed them.
  4. Incubated at 30°C.
  5. Measured intensity of green fluorescence at regular time intervals.
  1. E.Coli strain JW1908 or XL10Gold.
  2. Reaction Temparature 37 or 30.
  3. Cell number.

Results

Sender:JW1908, Receiver:JW1908, 37°C

Fig.　　
E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.

Sender:JW1908, Receiver:JW1908, 30°C

Fig.　　
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.

Senders:XL10Gold, Receiver:JW1908, 37°C

Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.

Senders:XL10Gold, Receiver:JW1908, 30°C

Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.


others

Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30,Receiver cells/Sender cells = 1.
Retrieved from "http://2008.igem.org/Team:Chiba/parts/BBa_K084007"