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Team:The University of Alberta/1 October 2008
From 2008.igem.org
(Difference between revisions)
(New page: *James **Eco R1 is digested as per Tom's request **run a gel: plasmid is ~2000bp so colonies 1-4, w/c are 21-25 are way undersized, that is we expect 5.5kb+2kb=approximally 7.5kb in size *...) |
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**run a gel: plasmid is ~2000bp so colonies 1-4, w/c are 21-25 are way undersized, that is we expect 5.5kb+2kb=approximally 7.5kb in size | **run a gel: plasmid is ~2000bp so colonies 1-4, w/c are 21-25 are way undersized, that is we expect 5.5kb+2kb=approximally 7.5kb in size | ||
**ETFA/B is matchs with expected size of 4300bp | **ETFA/B is matchs with expected size of 4300bp | ||
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| + | ==David== | ||
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| + | i transformed competent cells with the following ligations from yesterday | ||
| + | pTET+BisdA | ||
| + | pTET+BisdB | ||
Latest revision as of 03:35, 29 October 2008
- James
- Eco R1 is digested as per Tom's request
- run a gel: plasmid is ~2000bp so colonies 1-4, w/c are 21-25 are way undersized, that is we expect 5.5kb+2kb=approximally 7.5kb in size
- ETFA/B is matchs with expected size of 4300bp
David
i transformed competent cells with the following ligations from yesterday
pTET+BisdA pTET+BisdB
