Team:Brown/Project/Analysis

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{{BrownTemp}}
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[[Image:Conductance.png]]
 
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[[Image:Od measurements cell lysis test 2.png‎|center|thumb|550px|Optical Density WRT Time]]
[[Image:Od measurements cell lysis test 2.png‎|center|thumb|550px|Optical Density WRT Time]]
[[Image:Optical density measurement test 1.png|center|thumb|550px|Optical Density WRT Time]]
[[Image:Optical density measurement test 1.png|center|thumb|550px|Optical Density WRT Time]]
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==Optical Density==
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* Due to the nature of the Cell Lysis Cassette, Optical Densities can be used to determine when lysis has occurred.  The R gene (endolysin) of the cassette degrades the cell wall.  The solution becomes clear after lysis.  After two to three hours, the Optical Density of the cell cultures dropped, signifying the expression of the S,R,Rz genes.  The following graphs exhibit optical density trends during gene expression and resulting cell lysis and cell wall degradation. 
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[[Image:Conductance.png]]

Revision as of 04:31, 29 October 2008



Optical Density WRT Time
Optical Density WRT Time

Optical Density

  • Due to the nature of the Cell Lysis Cassette, Optical Densities can be used to determine when lysis has occurred. The R gene (endolysin) of the cassette degrades the cell wall. The solution becomes clear after lysis. After two to three hours, the Optical Density of the cell cultures dropped, signifying the expression of the S,R,Rz genes. The following graphs exhibit optical density trends during gene expression and resulting cell lysis and cell wall degradation.


Conductance.png