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EPF-Lausanne/6 October 2008
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==Cloning== | ==Cloning== | ||
| - | + | [[Image:epfl_gel-10-06-08.jpg|thumb|Agarose Gel: Clones of LuxI with RBS and Term. added by 2Step PCR]] | |
| - | Results of the plating of RBS-LuxI-Term: | + | '''Results of the plating of RBS-LuxI-Term''': |
40 colonies, and 6 on the vector autoligation. | 40 colonies, and 6 on the vector autoligation. | ||
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*8 colonies picked and miniprep'd. | *8 colonies picked and miniprep'd. | ||
*Ctrl. digestion with X,P. | *Ctrl. digestion with X,P. | ||
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Expected bands: | Expected bands: | ||
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Clones 2, 3, 5 and 8 are correct. | Clones 2, 3, 5 and 8 are correct. | ||
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| + | '''Cloning of A1 into B1:''' | ||
| + | |||
| + | Cloning was succesful. | ||
Latest revision as of 09:09, 29 October 2008
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Cloning
Results of the plating of RBS-LuxI-Term:
40 colonies, and 6 on the vector autoligation.
- 8 colonies picked and miniprep'd.
- Ctrl. digestion with X,P.
Expected bands:
- 2.1kb (vector pSB1A2)
- 800bp (luxI by 2step pcr)
Clones 2, 3, 5 and 8 are correct.
Cloning of A1 into B1:
Cloning was succesful.
