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Team:USTC/Results
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=== Correlation between GFP generation and cell density === | === Correlation between GFP generation and cell density === | ||
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| + | ::[[Image:USTC_OD-time.jpg |left]] | ||
| + | :::::::::::::::::[[Image:USTC_GFP-OD.jpg]] | ||
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| + | In order to know how the expression of signal molecules is related to cell density, we use GFP as the intermediate variable between the two variables. First, we determined the function of GFP according to the population of bacteria. Here, we have constructed the part with BioBricks R0040 and E0840. After ligating them together, we measured the amount of GFP expression and corresponding OD values and then worked out the correlation curve using Origin 7.5. | ||
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==Function Test == | ==Function Test == | ||
After GFP(LVA) cloned successfully,we want to test its function.We ligate it with lac promoter R0010,a strong RBS B0034 and teminator B0015. We transform the plasmid with these parts to E.coli strain TOP10 and induce the GFP express with 0.1mM IPTG. | After GFP(LVA) cloned successfully,we want to test its function.We ligate it with lac promoter R0010,a strong RBS B0034 and teminator B0015. We transform the plasmid with these parts to E.coli strain TOP10 and induce the GFP express with 0.1mM IPTG. | ||
:::::[[Image:USTC-result1.jpg|left|300px|thumb|fig 7.Cells grow on the left plate generate GFP after IPTG induced,celles on the right plate is negtive control.]] | :::::[[Image:USTC-result1.jpg|left|300px|thumb|fig 7.Cells grow on the left plate generate GFP after IPTG induced,celles on the right plate is negtive control.]] | ||
:::::::::::::::::::[[Image:USTC-result2.jpg|left|300px|thumb|fig 8.Cells that generate GFP in fluorescence microscope ]] | :::::::::::::::::::[[Image:USTC-result2.jpg|left|300px|thumb|fig 8.Cells that generate GFP in fluorescence microscope ]] | ||
Revision as of 09:43, 29 October 2008
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Correlation between GFP generation and cell density
In order to know how the expression of signal molecules is related to cell density, we use GFP as the intermediate variable between the two variables. First, we determined the function of GFP according to the population of bacteria. Here, we have constructed the part with BioBricks R0040 and E0840. After ligating them together, we measured the amount of GFP expression and corresponding OD values and then worked out the correlation curve using Origin 7.5.
Function Test
After GFP(LVA) cloned successfully,we want to test its function.We ligate it with lac promoter R0010,a strong RBS B0034 and teminator B0015. We transform the plasmid with these parts to E.coli strain TOP10 and induce the GFP express with 0.1mM IPTG.
fig 7.Cells grow on the left plate generate GFP after IPTG induced,celles on the right plate is negtive control.
fig 8.Cells that generate GFP in fluorescence microscope
