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Team:Rice University/Notebook/12 June 2008
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=Thursday 12 June= | =Thursday 12 June= | ||
*Taylor Stevenson | *Taylor Stevenson | ||
| - | **Transformation From New Registry (Paper Punches)-[[Team:Rice University/Electroporation|Electroporation]] and [[Team: | + | **Transformation From New Registry (Paper Punches)-[[Team:Rice University/Electroporation|Electroporation]] and [[Team:Rice_University/Heat_Shock|Heat Shock]] of [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13602 BBa_I13602] located on [http://partsregistry.org/Part:pSB1A2 pSB1A2] into TetR electrocompetent cells. |
*#Added 2uL of DNA solution obtained by soaking DNA punch in (10:1) Tris:EDTA buffer incubated @ 42*C for 20min. | *#Added 2uL of DNA solution obtained by soaking DNA punch in (10:1) Tris:EDTA buffer incubated @ 42*C for 20min. | ||
*#Electropulse parameters: 1.8 kV & 5.4 ms | *#Electropulse parameters: 1.8 kV & 5.4 ms | ||
Revision as of 17:19, 25 June 2008
Thursday 12 June
- Taylor Stevenson
- Transformation From New Registry (Paper Punches)-Electroporation and Heat Shock of [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13602 BBa_I13602] located on [http://partsregistry.org/Part:pSB1A2 pSB1A2] into TetR electrocompetent cells.
- Added 2uL of DNA solution obtained by soaking DNA punch in (10:1) Tris:EDTA buffer incubated @ 42*C for 20min.
- Electropulse parameters: 1.8 kV & 5.4 ms
- Plated 100uL and 20uL of transformed cells as well as 20uL of 1/100 and 1/10,000 dilutions in sterile 50% glycerol. Plates were cultured O/N.
- Result-no colonies were observed at any dilution
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