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Team:Rice University/Notebook/5 June 2008
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*#Grow VCS275 supA+ strain in Lambda media (LB supplemented with 0.2% w/v maltose and 10mM MgS04) @ 30*C O/N, shaking @ 250rpm. | *#Grow VCS275 supA+ strain in Lambda media (LB supplemented with 0.2% w/v maltose and 10mM MgS04) @ 30*C O/N, shaking @ 250rpm. | ||
*#Pellet cells @ 500xg for 10min, and re-suspend in 2x volume of 10mM MgS04. Aliquot into 200uL fractions. | *#Pellet cells @ 500xg for 10min, and re-suspend in 2x volume of 10mM MgS04. Aliquot into 200uL fractions. | ||
| - | *#Add serial dilutions of phage in SM buffer see [[ | + | *#Add serial dilutions of phage in SM buffer see [[packaging manual.pdf]] |
Revision as of 18:07, 25 June 2008
Thursday 5 June
- Selim Sheikh:
- Prepared gel electrophoresis of digestions from the previous day with 1Kb standard
(from right to left)
lane 1: 1Kb standard
lane 2: NEB lambda with HindIII
lane 3: NEB lambda with XhoI/HindIII)
- Taylor Stevenson
- Titered the phage stocks obtained from packaging the NEB lambda genome
- Grow VCS275 supA+ strain in Lambda media (LB supplemented with 0.2% w/v maltose and 10mM MgS04) @ 30*C O/N, shaking @ 250rpm.
- Pellet cells @ 500xg for 10min, and re-suspend in 2x volume of 10mM MgS04. Aliquot into 200uL fractions.
- Add serial dilutions of phage in SM buffer see packaging manual.pdf
