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Team:Rice University/Notebook/5 June 2008
From 2008.igem.org
Thursday 5 June
- Selim Sheikh:
- Prepared gel electrophoresis of digestions from the previous day with 1Kb standard
(from right to left)
lane 1: 1Kb standard
lane 2: NEB lambda with HindIII
lane 3: NEB lambda with XhoI/HindIII)
- Taylor Stevenson
- XL1-Blue MR cells were prepared for phage infection as specified in the phage packaging manual [1] and infected with phage at roughly a 1/10 pfu/cfu ratio. Resulting phage/bacteria mixture was incubated @ 37*C for 20 min and then streaked onto an LB plate. Plate was incubated @ 30*C O/N.
- Result-approximately 100 possibly lysogenic colonies grew on plate.
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