Biobrick
List of Biobricks:
BBa_K094100, BBa_K094101, BBa_K094102, BBa_K094103, BBa_K094104, BBa_K094105, BBa_K094106, BBa_K094110, BBa_K094111, BBa_K094112, BBa_K094113, BBa_K094120, BBa_K094130, BBa_K094141, BBa_K094150, BBa_K094151
BBa_K094100 ![](https://static.igem.org/mediawiki/2008/6/63/Biobrick_pic1.gif)
cheZ: This part contains the cheZ operon coding region without an rbs nor terminator. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.
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BBa_K094101 ![](https://static.igem.org/mediawiki/2008/b/bb/Biobrick_pic2.gif)
plambda(R-O12)-rbs-cheZ: This part contains a lambda promoter that is constitutively on and can be repressed by CI protein. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. This part lacks a terminator.
BBa_K094102 ![](https://static.igem.org/mediawiki/2008/d/d5/Biobrick_pic3.gif)
pTetR-rbs-cheZ: This part uses BBa_J61003 as a backbone and cheZ as the inserted gene. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement. The cheZ operon lacks a terminator.
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BBa_K094103 ![](https://static.igem.org/mediawiki/2008/c/c2/Biobrick_pic4.gif)
plux-rbs-cheZ-terminator: This is a cheZ gene that can be induced by luxR protein under high AHL concentration. cheZ protein is responsible for the dephosphorylation of cheY protein in bacteria flagella movement.
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BBa_K094104 ![](https://static.igem.org/mediawiki/2008/4/4e/Biobrick_pic5.gif)
pLacI/ara-1-rbs-mRFP-double terminator: This part is adapted from part BBa_J04450 by changing the promoter pLacI to promoter pLacI/ara-1. This part can test the leaky of promoter plac/ara-1.
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BBa_K094105 ![](https://static.igem.org/mediawiki/2008/9/9d/Biobrick_pic6.gif)
plambda(R-O12)-rbs-cheZ:-rbs-mRFP1-double terminator: This part can be used to measure the gene expression of cheZ under the control of promoter plambda(R-O12).
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BBa_K094106 ![](https://static.igem.org/mediawiki/2008/9/9b/Biobrick_pic7.gif)
Plux-rbs-CI-terminator-plambda-rbs-cheZ: This part encodes a CI protein that is used to repress lambda promoter. The part is controlled by the lux promoter that can be induced by luxR protein under high AHL concentration. In this part, the expression of cheZ is repressed by increasing AHL concentration in the environment in the presence of luxR protein. In other circumstances, the cheZ gene express constitutively.
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BBa_K094110 ![](https://static.igem.org/mediawiki/2008/9/9b/Biobrick_pic8.gif)
cheY*: This is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This part contains the coding region only.
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BBa_K094111![](https://static.igem.org/mediawiki/2008/0/07/Biobrick_pic9.gif)
pTetR-rbs-cheY*: This part uses BBa_J61003 as a backbone and cheY* as the inserted gene. cheY is the gene coding for cheY protein which controls flagella movement. This sequence, however, is mutated to give a mutant cheY protein which will not respond to the presence of cheZ protein. This part lacks a terminator.
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BBa_K094112 ![](https://static.igem.org/mediawiki/2008/3/33/Biobrick_pic10.gif)
luxR-rbs-luxI-terminator: : The bacterial luciferin-luciferase system is encoded by a set of genes labelled the Lux operon. In V. fischeri five such genes (LuxCDABE) have been identified as active in the emission of visible light, and two genes (LuxR and LuxI) are involved in regulating the operon. This part can be utilized as a part of quorum sensing system.
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BBa_K094113![](https://static.igem.org/mediawiki/2008/1/1b/Biobrick_pic11.gif)
lacZ: This is a reporter gene that codes for beta galactosidase.
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BBa_K094120 ![](https://static.igem.org/mediawiki/2008/b/b4/Biobrick_pic12.gif)
pLacI/ara-1: This promoter can be repressed by lacI protein and induced by either IPTG or arabinose.
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BBa_K094130 ![](https://static.igem.org/mediawiki/2008/c/c6/Biobrick_pic13.gif)
rbs-mRFP1-terminator: This part is adapted from part BBa_J04450, with the pLacI promoter deleted. This part can serve as promoter efficiency test kit.
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BBa_K094141 <![](https://static.igem.org/mediawiki/2008/c/cc/Biobrick_pic14.gif)
pLacIq-rbs-LacIq-terminator: LacIQ can repress promoter lac. This part contains a mutated pLacIq promoter which enables high level of transcription. This ensures effective repression of pLacI promoter that might be present.
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BBa_K094150 ![](https://static.igem.org/mediawiki/2008/0/0f/Biobrick_pic15.gif)
pLacI/ara-1-rbs-luxI-luxR-terminator: This is a quorum sensing composition. Induced by IPTG or arabinose, this part can establish a quorum sensing system, with the luxI protein producing AHL and luxR protein as the AHL density sensor.
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BBa_K094151 ![](https://static.igem.org/mediawiki/2008/4/4d/Biobrick_pic16.gif)
pLacI/ara-1-rbs-luxI-terminator: The luxI operon is controlled by promoter LacI/ara-1.
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