Meetings

From 2008.igem.org

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<br><br>
<br><br>
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'''Sept. 24th. 2008'''<br>
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...<br>
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'''Sept. 29th 2008'''<br>
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...<br>
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'''Oct. 7th 2008''''<br>
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...<br>
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'''Oct. 17th 2008'''<br><br>
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'''participants:'''<br>
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Philipp Mappes, Katja Arndt, Kristian Müller, Normann Kilb, Robert Gawlik, Michael Kneib
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<br><br>
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'''Final report'''
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<ul>
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<li> structure equal to a scientific paper containing the parts Abstract, Introduction, Materials and Methods, Results</li>
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<li> fusion of all sections we worked in: Origami, AFM, FACS, CA<sup>2+</sup>/Fura measuremt, cell cultures, modeling, ethics</li>
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<li> quotation of literature through autor and year of publication in the text (Max Muster, 2007), full quotation in references in the end</li>
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<li> deadline Friday Oct. 24th 2008</li>
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</ul><br>
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'''official wikipediasite'''
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<ul>
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<li> continue the labjournal on the official page only</li>
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<li> not too much linking: maintenance of the flow of information</li>
 +
<li> all embedded files should start with "Freiburg2008"</li>
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</ul><br>
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'''iGEM parts'''
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<ul>
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<li>uploading the parts starting on Monday Oct. 20th 2008</li>
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</ul>
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<br>
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'''Oct. 21th 2008'''<br>
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'''participants:<br>'''
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Moritz Busaker, Philipp Mappes, Kristian Müller, Normann Kilb, Robert Gawlik, Michael Kneib, Kathrin Pieper, Simone Weber<br>
 +
 +
<br>
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'''Cloning:<br>'''
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At least - All the constructes are cloned!!!<br>
 +
<br>
 +
construcs: <br>
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<br>
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pMA - singalpeptide – scFv-anti-NIP – gggs-Linker – transmembran region - '''X''' –  pMA<br><br>
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'''X :'''
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* bla1(1/2 beta-Lactamase)-<br>
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* bla2 (1/2 beta-Lactamase) –<br>
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* split-linker-C-CFP(Cerutan) –<br>
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* N-CFP – <br>
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* split-linker-C-GFP (split venus)– '''--------> Hi Philipp oder Robert!!! könnt ihr von bla1 bis Luciferase2 ein Stück einziehen?! Habs nicht hin bekommen''' <br>
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* N-GFP –<br>
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* luciferase 1 (58) –<br>
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*luciferase 2 (57) –<br>
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<br>
 +
<br>
 +
'''Next steps:''' <br>
 +
1) We want to test, if the transmembran region really is located in the membrane<br>
 +
Therefore we want to clone a constructe which expresses a YFP on the cytoplasmic side of the receptor.<br>
 +
Construct:<br>
 +
-  singalpeptide  –  scFv-anti-NIP  –  gggs-Linker  –  transmembran region  - split-linker  -  bla1  - YFP -<br>
 +
<br>
 +
2) We also want to test, if we able to bringe the receptors together by using a NIP coupled peptide.<br>
 +
Therefore we need the peptides from Schamels group -> Norman will ask them.<br>
 +
<br>
 +
3) Origami-We want to try if the Origami are able to bring two or more receptors together.<br>
 +
Have to produce new Origami -> Michael and Norman<br>
 +
<br>
 +
Next meeting: Oct. 23th 2008<br>
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<br>
}}
}}

Revision as of 15:24, 29 October 2008


Freiburg2008 small header.gif



Home

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CoLABoration
_meetings




Sept. 24th. 2008
...


Sept. 29th 2008
...


Oct. 7th 2008'
...


Oct. 17th 2008

participants:
Philipp Mappes, Katja Arndt, Kristian Müller, Normann Kilb, Robert Gawlik, Michael Kneib

Final report

  • structure equal to a scientific paper containing the parts Abstract, Introduction, Materials and Methods, Results
  • fusion of all sections we worked in: Origami, AFM, FACS, CA2+/Fura measuremt, cell cultures, modeling, ethics
  • quotation of literature through autor and year of publication in the text (Max Muster, 2007), full quotation in references in the end
  • deadline Friday Oct. 24th 2008

official wikipediasite

  • continue the labjournal on the official page only
  • not too much linking: maintenance of the flow of information
  • all embedded files should start with "Freiburg2008"

iGEM parts

  • uploading the parts starting on Monday Oct. 20th 2008



Oct. 21th 2008

participants:
 Moritz Busaker, Philipp Mappes, Kristian Müller, Normann Kilb, Robert Gawlik, Michael Kneib, Kathrin Pieper, Simone Weber


Cloning:
 At least - All the constructes are cloned!!!

construcs:

pMA - singalpeptide – scFv-anti-NIP – gggs-Linker – transmembran region - X – pMA

X :

  • bla1(1/2 beta-Lactamase)-
  • bla2 (1/2 beta-Lactamase) –
  • split-linker-C-CFP(Cerutan) –
  • N-CFP –
  • split-linker-C-GFP (split venus)– --------> Hi Philipp oder Robert!!! könnt ihr von bla1 bis Luciferase2 ein Stück einziehen?! Habs nicht hin bekommen
  • N-GFP –
  • luciferase 1 (58) –
  • luciferase 2 (57) –



Next steps:
1) We want to test, if the transmembran region really is located in the membrane
Therefore we want to clone a constructe which expresses a YFP on the cytoplasmic side of the receptor.
Construct:
- singalpeptide – scFv-anti-NIP – gggs-Linker – transmembran region - split-linker - bla1 - YFP -

2) We also want to test, if we able to bringe the receptors together by using a NIP coupled peptide.
Therefore we need the peptides from Schamels group -> Norman will ask them.

3) Origami-We want to try if the Origami are able to bring two or more receptors together.
Have to produce new Origami -> Michael and Norman

Next meeting: Oct. 23th 2008

Freiburg08 FT3.png

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