Latest revision as of 03:50, 26 June 2008

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Things we did today

Krystle & Margaret

Krystle

Norman

Krystle, Norman, & Margaret

Note to self: if I have weird cancers in strange places, its probably because I was playing with these carcinogens. - KS

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 5: / Line 5: @@
 ===Alkaline Lysis Miniprep===
 :<strong> Krystle & Margaret </strong>
+:* Plasmid prep was performed according to the experimental protocol
+:* Plasmid prep was performed with:
+{| border="1"
+|+ '''BioBricks'''
+!part number
+!antibx media strength
+!source organism
+|-
+|E0040 (GFP)
+|rowspan= "5" align="center" |amp100
+|DH5&alpha
+|-
+|J04430 (lac+GFP)
+|DH5&alpha
+|-
+|B0034 (RBS)
+|GP's super competent cells
+|-
+|B0024 (TT)
+|DH5&alpha
+|}
+{| border="1"
+|+ '''Tool Plasmids'''
+! type
+!antibx media strength
+!source organism
+|-
+|align="center" |pUC18
+|rowspan= "2" align="center" | amp100
+|rowspan= "2" align="center" |DH5&alpha
+|-
+|align="center" |pBLUSCRIPT
+|-
+|align="center" |RP1
+|align="center" |kan50
+|align="center" |E. coli
+|}
-:* Plasmid prep was performed with Biobricks (E0040 (GFP) with amp100 from DH5alpha, J04430 (lac+GFP) with amp100 from DH5alpha, B0034 (RBS) with amp100 from GP's super competent cells, and B0024 (TT)with amp100 from DH5alpha) and plasmids (pUC18 with amp100 from DH5alpha, pBLUSCRIPT with amp100 from DH5alpha, and RP4 with Kan 50 from E. coli HB101).
 ===Cryo-Stocks===
 :<strong>Krystle</strong>
 :* Made cryo-stocks of pBLUSCRIPT, pUC18, and B0024.
+===Run Gel for pRL1383a plasmid Prep===
+:<strong>Norman</strong>
+:*See experiment for details.
 == Drylab Work ==