Team:Edinburgh/Plan/Beta-Carotene

From 2008.igem.org

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(β-carotene synthesis)
(Directly involved genes from P. ananatis)
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# Lycopene is produced from phytoene by phytoene desaturase ([https://2008.igem.org/Team:Edinburgh/crtI '''''crtI''''']).
# Lycopene is produced from phytoene by phytoene desaturase ([https://2008.igem.org/Team:Edinburgh/crtI '''''crtI''''']).
# Finally, lycopene β-cyclase ([https://2008.igem.org/Team:Edinburgh/crtY '''''crtY''''']) cyclises lycopene to produce β-carotene.
# Finally, lycopene β-cyclase ([https://2008.igem.org/Team:Edinburgh/crtY '''''crtY''''']) cyclises lycopene to produce β-carotene.
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See figure 1 for structure details.
== Indirectly involved genes from ''E. coli'' ==
== Indirectly involved genes from ''E. coli'' ==

Revision as of 16:18, 29 October 2008

Contents

β-carotene synthesis

β-carotene is produced from the products of glycolysis, as can be seen in the overview figure. We have concentrated on two areas of β-carotene for our project. The first involves transfering carotenoid synthesis genes from Pantoea ananatis, a member of the proteobacteria naturally capable of producing β-carotene. The second involves upregulating the glycolysis pathways in E. coli in order to concentrate more energy into making β-carotene.

Directly involved genes from P. ananatis

  1. Geranyl diphosphate synthase (crtE) converts the substrates farnesyl diphosphate and isopentyl diphosphate into geranyl geranyl diphosphate.
  2. Geranyl geranyl diphosphate is then converted into phytoene by phytoene synthase (crtB).
  3. Lycopene is produced from phytoene by phytoene desaturase (crtI).
  4. Finally, lycopene β-cyclase (crtY) cyclises lycopene to produce β-carotene.

See figure 1 for structure details.

Indirectly involved genes from E. coli

Overview

Edinburgh-Beta-carotene.jpg
In blue are the genes which we manipulated.