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Revision as of 19:32, 29 October 2008 (view source) Takahiro (Talk | contribs) (→Team:Input) ← Older edit		Revision as of 19:33, 29 October 2008 (view source) Takahiro (Talk | contribs) (→Team:Input) Newer edit →
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	'''[[Team:Chiba/protocol/transformation|Transformation]]'''		'''[[Team:Chiba/protocol/transformation|Transformation]]'''

---

Revision as of 19:33, 29 October 2008

>Home | Notebook

19 August 2008 <|> 21 August 2008

Contents 1 Laboratory work 1.1 Team:Input 1.2 Team:Communication 1.3 Team:Output

Laboratory work

Team:Input

PCR

• [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)(1)
• [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2008)(2)
• [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)(3)
• [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2008)(4)
• [http://partsregistry.org/Part:BBa_E0612 BBa_E0612](2008)(5)

>

Sample No.	1	2	3	4	5
DNA tamplate	1	1	1	1	1
FW primer	5	5	5	5	5
VR primer	5	5	5	5	5
dNTPmix	10	10	10	10	10
thermo pol buffer	10	10	10	10	10
DNA pol(VENT)	1	1	1	1	1
dH2O	68	68	68	68	68
TOTAL	100	100	100	100	100

-->Gel Check

>

Sample No.	1	2	3	4	5
Sample DNA	1	1	1	1	1
loading dye	1	1	1	1	1
dH2O	4	4	4	4	4
Total	6	6	6	6	6

-->1,2,3,4,5:none

Transformation

• [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2007)
• [http://partsregistry.org/Part:BBa_R0051 BBa_R0051](2006)
• [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2007)
• [http://partsregistry.org/Part:BBa_J06650 BBa_J06650](2006)
• [http://partsregistry.org/Part:BBa_J22136 BBa_J22136](2007)
• [http://partsregistry.org/Part:BBa_J22141 BBa_J22141](2007)-->only one colony

-->BBa_J22141のみコロニーがほとんど生えていなかった。(１個のみ)

Team:Communication

(18/8)--->Mini prep

• [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007)
• [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006)
• [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007)
• [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007)
• [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006)
• [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
• [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
• [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
• [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)

--->Gel Check

Sample DNA 3 Loading Dye 2 dH2O 7 TOTAL 12μL From left; [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> None

Sample DNA 10 Loading Dye 4 dH2O 10 TOTAL 24μL From left; [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007) -> None [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006) -> OK [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007) -> None [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007) -> OK [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006) -> OK

Sample DNA 3 Loading Dye 2 dH2O 7 TOTAL 12μL From left; [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK

--->Digestion test

• [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
• [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
• [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
• [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)

Sample No	1	2	3	4	5	6	7	8
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)	3	-	-	-	3	-	-	-
[http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)	-	3	-	-	-	3	-	-
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)	-	-	3	-	-	-	3	-
[http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)	-	-	-	3	-	-	-	3
EcoRⅠ	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1
PstⅠ	-	-	-	-	0.1	0.1	0.1	0.1
Buffer EcoRⅠ	1	1	1	1	1	1	1	1
BSA	1	1	1	1	1	1	1	1
dH2O	4.9	4.9	4.9	4.9	4.8	4.8	4.8	4.8
TOTAL	10	10	10	10	10	10	10	10

--->Gel Check

Sample DNA 3 Loading Dye 2 dH2O 7 TOTAL 12μL From left; (Single Digestion 1~4) [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK (Double Digestion 1~4) [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006) -> OK [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007) -> OK [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006) -> OK [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007) -> OK

Team:Output

Transformation

• [http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2007)-->colony
• [http://partsregistry.org/Part:BBa_I712019 BBa_I712019](2008)-->no colony
• [http://partsregistry.org/Part:BBa_J33202 BBa_J33202](2007)-->colony
• [http://partsregistry.org/Part:BBa_E2030 BBa_E2030](2007)-->not so good
• YFP(P-GEX)-->colony(Amp)
• [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)-->colony
• [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007)-->colony

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