Team:Paris/Modeling/Workflow Example
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{{Paris/Header|Characterization Approach : Generic Workflow on an Example}} | {{Paris/Header|Characterization Approach : Generic Workflow on an Example}} | ||
- | Here, we will explain the generic protocol that leads to the modelisation "by characterization", of one of the parts of our system. The choosen example is '''the | + | Here, we will explain the generic protocol that leads to the modelisation "by characterization", of one of the parts of our system. The choosen example is '''the activity of the promoter ''pFliL'' ''' (that leads to the production of the '''Fluorescent Protein FP1''') '''in function of the transcription factors ''FlhDC'' and ''FliA'' '''. |
We know that ''the hexamere FlhD<sub>4</sub>C<sub>2</sub>'' and the protein ''FliA'' act both on the promoter ''pFliL'' as inducers... how do we evaluate this phenomenon? <br> | We know that ''the hexamere FlhD<sub>4</sub>C<sub>2</sub>'' and the protein ''FliA'' act both on the promoter ''pFliL'' as inducers... how do we evaluate this phenomenon? <br> | ||
As it is illustrated below; starting from ''molecular reactions'' '''(1)''', we develop a ''model of our system'' '''(2)''', and then we identify the ''parameters'' '''(3)''' that needs to be estimated. Essentially, it amounts to estimate ''Promoters Activities as functions of their Transcription Factors''. To do so, we propose ''constructs'' '''(4)''', ''protocols'' '''(5)''' and ''programs'' '''(6)'''. | As it is illustrated below; starting from ''molecular reactions'' '''(1)''', we develop a ''model of our system'' '''(2)''', and then we identify the ''parameters'' '''(3)''' that needs to be estimated. Essentially, it amounts to estimate ''Promoters Activities as functions of their Transcription Factors''. To do so, we propose ''constructs'' '''(4)''', ''protocols'' '''(5)''' and ''programs'' '''(6)'''. |
Revision as of 19:40, 29 October 2008