Team:Chiba/Calendar-Home/23 September 2008

From 2008.igem.org

(Difference between revisions)

Revision as of 20:03, 29 October 2008

>Home | Notebook

22 September 2008 <|> 24 September 2008

Laboratory work

Team:Communication

time-delay test
- transformants
  - [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
  - [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
  - [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
  - [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
  - [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084012　 BBa_K084012]
  - [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084014　 BBa_K084014]
- E.coli strain
  Senders:XL10GOLD
  Receiver:JW1908
- reaction temparature
  37°C and 30°C

Result(at 37°C)
- Receiver cells/Sender cells = 1

File:Chiba communication 0923 01.xls

- Receiver cells/Sender cells = 10

Result(at 30°C)
- Receiver cells/Sender cells = 1

- Receiver cells/Sender cells = 10

Team:Output

Digestion test
BBa_E0240

DNA(μL)	2
EcoRI(μL)	0.5
PstI(μL)	0.5
BSA(×10)	1
EcoRI Buffer(&time;10)	1
dH₂O(μL)	5
TOTAL(μL)	10

TRANSFORMATION
BBa_I6107
BBa_I6206
BBa_I6101
BBa_I6042
BBa_I0462

Digestion
Vector:BBa_R0062:1
Insert:BBa_E0240:2
Vector:BBa_I7100:3
Insert:BBa_R0062:4
Insert:BBa_J37033:5

</tr>

Sample No	1	2	3	4	5
DNA(μL)	25	25	25	25	25
EcoRI(μL)	-	-	1	1	-
SpeI(μL)	1	-	1	1	-
PstI(μL)	1	1	1	-	1
XbaI(μL)	-	1	-	-	1
BSA(×10)(μL)	4	4	4	-	4
EcoRI Buffer(&time;10)(μL)	-	-	4	4	-
Buffer 2(μL)	4	-	-	-	-
Buffer 3(μL)	-	4	-	-	4
dH₂O(μL)	5	5	5	9	5
TOTAL(μL)	40	40	40	40	40

->1 hour 37°C
->SAP 1μL(Vector only) ->1 hour 37°C ->30 minutes 65°C
->Zymo ->10μL
Gel check

DNA(μL)	1
Loading Dye(μL)	1
dH₂O(μL)	4
TOTAL(μL)	6

->3&4&5のLigationはしない

@@ Line 16: / Line 16: @@
 **reaction temparature<br>37°C and 30°C
-*Result(at 37°C)
+*Result(at 37&deg;C)
 **Receiver cells/Sender cells = 1
 [[Image:Chiba_communication_0923_01.gif]]
@@ Line 22: / Line 22: @@
 **Receiver cells/Sender cells = 10
-*Result(at 30°C)
+*Result(at 30&deg;C)
 **Receiver cells/Sender cells = 1
@@ Line 33: / Line 33: @@
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">DNA</td>
+<td width="257">DNA(&mu;L)</td>
 <td>2</td>
 </tr>
 <tr>
-<td>EcoRⅠ</td>
+<td>EcoRI(&mu;L)</td>
 <td>0.5</td>
 </tr>
 <tr>
-<td>PstⅠ</td>
+<td>PstI(&mu;L)</td>
 <td>0.5</td>
 </tr>
 <tr>
-<td>BSA(x10)</td>
+<td>BSA(&times;10)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>EcoRⅠ Buffer(x10)</td>
+<td>EcoRI Buffer(&time;10)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>5</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
 <td>10</td>
 </tr>
@@ Line 71: / Line 71: @@
 <br>Digestion
-<br>Vector:BBa_R0062①
+<br>Vector:BBa_R0062:1
-<br>Insert:BBa_E0240②
+<br>Insert:BBa_E0240:2
-<br>Vector:BBa_I7100③
+<br>Vector:BBa_I7100:3
-<br>Insert:BBa_R0062④
+<br>Insert:BBa_R0062:4
-<br>Insert:BBa_J37033⑤
+<br>Insert:BBa_J37033:5
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
 <td width="257">Sample No</td>
-<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
+<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td>
 </tr>
 <tr>
-<td>DNA</td>
+<td>DNA(&mu;L)</td>
 <td>25</td><td>25</td><td>25</td><td>25</td><td>25</td>
 </tr>
 <tr>
-<td>EcoRⅠ</td>
+<td>EcoRI(&mu;L)</td>
 <td>-</td><td>-</td><td>1</td><td>1</td><td>-</td>
 </tr>
 <tr>
-<td>SpeⅠ</td>
+<td>SpeI(&mu;L)</td>
 <td>1</td><td>-</td><td>1</td><td>1</td><td>-</td>
 </tr>
 <tr>
-<td>PstⅠ</td>
+<td>PstI(&mu;L)</td>
 <td>1</td><td>1</td><td>1</td><td>-</td><td>1</td>
 </tr>
 <tr>
-<td>XbaⅠ</td>
+<td>XbaI(&mu;L)</td>
 <td>-</td><td>1</td><td>-</td><td>-</td><td>1</td>
 </tr>
-<td>BSA(x10)</td>
+<td>BSA(&times;10)(&mu;L)</td>
 <td>4</td><td>4</td><td>4</td><td>-</td><td>4</td>
 </tr>
 <tr>
-<td>EcoRⅠ Buffer(x10)</td>
+<td>EcoRI Buffer(&time;10)(&mu;L)</td>
 <td>-</td><td>-</td><td>4</td><td>4</td><td>-</td>
 </tr>
 <tr>
-<td>Buffer 2</td>
+<td>Buffer 2(&mu;L)</td>
 <td>4</td><td>-</td><td>-</td><td>-</td><td>-</td>
 </tr>
 <tr>
-<td>Buffer 3</td>
+<td>Buffer 3(&mu;L)</td>
 <td>-</td><td>4</td><td>-</td><td>-</td><td>4</td>
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>5</td><td>5</td><td>5</td><td>9</td><td>5</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
 <td>40</td><td>40</td><td>40</td><td>40</td><td>40</td>
 </tr>
 </table>
-<br>->1hour 37℃
+<br>->1 hour 37&deg;C
-<br>->SAP 1μl(Vector only) ->1hour 37℃ ->30min 65℃
+<br>->SAP 1&mu;L(Vector only) ->1 hour 37&deg;C ->30 minutes 65&deg;C
-<br>->Zymo  ->10μl
+<br>->Zymo  ->10&mu;L
-<br>Gel cheack
+<br>Gel check
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">DNA</td>
+<td width="257">DNA(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(&mu;L)</td>
 <td>1</td>
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O(&mu;L)</td>
 <td>4</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(&mu;L)</td>
 <td>6</td>
 </tr>
 </table>
-<br>->③&④&⑤のLigationはしない
+<br>->3&4&5のLigationはしない