Team:Chiba/Calendar-Home/31 August 2008

From 2008.igem.org

(Difference between revisions)

Latest revision as of 20:41, 29 October 2008

>Home | Notebook

30 August 2008 <|> 1 September 2008

Laboratory work

Team:Input

no work

Team:Communication

Transformation

Competent Cells : XL10Gold

[http://partsregistry.org/Part:BBa_K084007 BBa_K084007](Plac+RBS+LasI)
[http://partsregistry.org/Part:BBa_K084008 BBa_K084008](Plac+RBS+RhlI)

---> We saved these plates with a refrigerator.

Digestion

[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)

Sample No.	1~3	4
Sample DNA(μL)	12	10
PstⅠ(μL)	0.2	0.2
XbaⅠ(μL)	0.2	-
SpeⅠ(μL)	-	0.4
Buffer 2(μL)	-	1.5
Buffer 3(μL)	2	-
BSA(μL)	2	1.5
dH₂O(μL)	3.6	1.4
TOTAL(μL)	20	15

--->(1/9)Gel Check

(30/8)--->Mini prep

--->Digestion test

[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5
[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)

Sample	Single Digesiton 2~5	Double Digestion 2~5	Single Digestion T9002(1)	Single Digesiton T9002(2)	Double Digestion T9002(1)(2)
Sample DNA(μL)	1	3	3	1	3
XbaI(μL)	0.1	0.1	0.1	0.1	0.1
SpeI(μL)	-	0.1	-	-	0.1
Buffer 2(μL)	0.9	0.8	0.9	0.9	0.8
BSA(μL)	1	1	1	1	1
dH₂O(μL)	7	5	5	7	5
TOTAL(μL)	10	10	10	10	10

--->Gel Check

Sample No.	[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1)(2)	Single & Double Digestion
Sample DNA(μL)	1	10
Loading Dye(μL)	1	2
dH₂O(μL)	4	-
TOTAL(μL)	6	12

From Left

Single Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
Double Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)

Team:Output

Digestion

[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](1)
[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2)

Sample No.	1	2
DNA	10	10
SpeI	1	0
PstI	1	1
XbaI	0	1
BSA(x100)	0.5	0.5
Buffer2	5	0
Buffer3	0	5
dH₂O	28.5	28.5
TOTAL	50μl	50μl

-->left for 3 hours at 37 °C.

-->(1)20μl refine

-->(2)20μl refine

@@ Line 9: / Line 9: @@
 ===Team:Communication===
 :'''[[Team:Chiba/protocol/transformation|Transformation]]'''
-:Competent Cells : XL10G
+:Competent Cells : XL10Gold
-::*[http://partsregistry.org/Part:BBa_K084007 BBa_K084007(Plac+RBS+LasI)]
+::*[http://partsregistry.org/Part:BBa_K084007 BBa_K084007](Plac+RBS+LasI)
-::*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008(Plac+RBS+RhlI)]
+::*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008](Plac+RBS+RhlI)
 ::---> We saved these plates with a refrigerator.
@@ Line 28: / Line 28: @@
 </tr>
 <tr>
-<td>Sample DNA</td><td>12</td><td>10</td>
+<td>Sample DNA(μL)</td><td>12</td><td>10</td>
 </tr>
 <tr>
-<td>PstⅠ</td>
+<td>PstⅠ(μL)</td>
 <td>0.2</td><td>0.2</td>
 </tr>
 <tr>
-<td>XbaⅠ</td><td>0.2</td><td>-</td>
+<td>XbaⅠ(μL)</td><td>0.2</td><td>-</td>
 </tr>
 <tr>
-<td>SpeⅠ</td><td>-</td><td>0.4</td>
+<td>SpeⅠ(μL)</td><td>-</td><td>0.4</td>
 </tr>
 <tr>
-<td>Buffer 2</td>
+<td>Buffer 2(μL)</td>
 <td>-</td><td>1.5</td>
 </tr>
 <tr>
-<td>Buffer 3</td>
+<td>Buffer 3(μL)</td>
 <td>2</td><td>-</td>
 </tr>
 <tr>
-<td>BSA</td>
+<td>BSA(μL)</td>
 <td>2</td><td>1.5</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>3.6</td><td>1.4</td></tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>20</td><td>15</td>
 </tr>
@@ Line 69: / Line 69: @@
 :--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
-::*Plac+RBS+RhlI Sample No.2~5
+::*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5
-::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
+::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
 <table width="600" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
 <td width="257">Sample</td>
-<td>Single Digesiton 2~5</td><td>Double Digestion 2~5</td><td>Single Digestion T9002①</td><td>Single Digesiton T9002②</td><td>Double Digestion T9002①②</td>
+<td>Single Digesiton 2~5</td><td>Double Digestion 2~5</td><td>Single Digestion T9002(1)</td><td>Single Digesiton T9002(2)</td><td>Double Digestion T9002(1)(2)</td>
 </tr>
 <tr>
-<td>Sample DNA</td><td>1</td><td>3</td><td>3</td><td>1</td><td>3</td>
+<td>Sample DNA(μL)</td><td>1</td><td>3</td><td>3</td><td>1</td><td>3</td>
 </tr>
 <tr>
-<td>XbaⅠ</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
+<td>XbaI(μL)</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
 </tr>
 <tr>
-<td>SpeⅠ</td><td>-</td><td>0.1</td><td>-</td><td>-</td><td>0.1</td>
+<td>SpeI(μL)</td><td>-</td><td>0.1</td><td>-</td><td>-</td><td>0.1</td>
 </tr>
 <tr>
-<td>Buffer 2</td>
+<td>Buffer 2(μL)</td>
 <td>0.9</td><td>0.8</td><td>0.9</td><td>0.9</td><td>0.8</td>
 </tr>
 <tr>
-<td>BSA</td>
+<td>BSA(μL)</td>
 <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 </tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>7</td><td>5</td><td>5</td><td>7</td><td>5</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
 </tr>
@@ Line 113: / Line 113: @@
 <tr>
 <td width="257">Sample No.</td>
-<td>Plac+RBS+RhlI Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①②
+<td>[http://partsregistry.org/Part:BBa_K084008 BBa_K084008] Sample No.2~5, [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1)(2)
 </td><td>Single & Double Digestion</td>
 </tr>
 <tr>
-<td>Sample DNA</td>
+<td>Sample DNA(μL)</td>
 <td>1</td><td>10</td>
 </tr>
 <tr>
-<td>Loading Dye</td>
+<td>Loading Dye(μL)</td>
 <td>1</td><td>2</td></tr>
 <tr>
-<td>dH<sub>2</sub>O</td>
+<td>dH<sub>2</sub>O(μL)</td>
 <td>4</td><td>-</td>
 </tr>
 <tr>
-<td>TOTAL</td>
+<td>TOTAL(μL)</td>
 <td>6</td><td>12</td>
 </tr>
 </table>
 :From Left
-::*Single Digestion Plac+RBS+RhlI 2~5
+::*Single Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
-::*Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
+::*Single Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
-::*Double Digestion Plac+RBS+RhlI 2~5
+::*Double Digestion [http://partsregistry.org/Part:BBa_K084008 BBa_K084008] 2~5
-::*Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
+::*Double Digestion [http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
-::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002]①、②
+::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](1),(2)
 |}
 ===Team:Output===
 [[Team:Chiba/protocol/digestion|Digestion]]
-*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]①
+*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](1)
-*PCR product([http://partsregistry.org/Part:BBa_J52008 BBa_J52008])②
+*[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](2)
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
 <td width="257">Sample No.</td>
-<td>①</td><td>②</td>
+<td>1</td><td>2</td>
 </tr>
 <tr>
-<td>DNA </td>
+<td>DNA</td>
 <td>10</td><td>10</td>
 </tr>
 <tr>
-<td>SpeⅠ</td>
+<td>SpeI</td>
 <td>1</td><td>0</td>
 </tr>
 <tr>
-<td>PstⅠ</td>
+<td>PstI</td>
 <td>1</td><td>1</td>
 </tr>
 <tr>
-<td>XbaⅠ</td>
+<td>XbaI</td>
 <td>0</td><td>1</td>
 </tr>
@@ Line 188: / Line 185: @@
 <tr>
 <td>TOTAL</td>
-<td>50</td><td>50</td>
+<td>50μl</td><td>50μl</td>
 </tr>
 </table>
--->37℃ 3hour
+-->left for 3 hours at 37 °C.
--->①20μl refine
+-->(1)20μl refine
--->②20μl refine
+-->(2)20μl refine