Team:Warsaw/Calendar-Main/24 July 2008
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+ | <h3>Cloning of protein Z DNA to <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b-OmpA-omega</a> in place of OmpA</h3><h4>Paweł</h4> | ||
+ | <ol><li>Results of ligation: 6 colonies grown.</li> | ||
+ | <li>Each colony cultured overnight in LB + ampicillin.</li></ol> | ||
+ | <h3>Cloning of protein A DNA to OmpA constructs</h3><h4>Michał K.</h4> | ||
+ | <ol> | ||
+ | <li>Colony PCR on colonies from plates with transformations <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-A-omega>pACYC177+OmpA_A_omega</a>. Primers used: | ||
+ | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a>.</li><li> Gel electrophoresis.</li> | ||
+ | <li>Confirmed transformant colonies inoculated to liquid LB with kanamycin. </li></ol> | ||
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+ | <h3>Preparation of chemocompetent bacteria</h3> | ||
+ | <h4>Antoni</h4> | ||
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+ | <p>Preparation of <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> bacteria <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htmtop10">TOP10</a>.</p> | ||
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Latest revision as of 21:22, 29 October 2008
Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpAPaweł
Cloning of protein A DNA to OmpA constructsMichał K.
Preparation of chemocompetent bacteriaAntoniPreparation of chemocompetent bacteria E. coli TOP10.
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