Team:Warsaw/Calendar-Main/24 July 2008

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<h3>Preparation of chemocompetent <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a></h3>
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<h3>Preparation of chemocompetent bacteria</h3>
<h4>Antoni</h4>
<h4>Antoni</h4>

Latest revision as of 21:22, 29 October 2008

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Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA

Paweł

  1. Results of ligation: 6 colonies grown.
  2. Each colony cultured overnight in LB + ampicillin.

Cloning of protein A DNA to OmpA constructs

Michał K.

  1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega. Primers used: AL+SacI and AP+NotI.
  2. Gel electrophoresis.
  3. Confirmed transformant colonies inoculated to liquid LB with kanamycin.

Preparation of chemocompetent bacteria

Antoni

Preparation of chemocompetent bacteria E. coli TOP10.

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