Edinburgh/18 August 2008

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:::: '''[[Edinburgh/17_August_2008|< Previous Entry]]'''
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'''[[Edinburgh/17_August_2008|< Previous Entry]] | [[Team:Edinburgh/Notebook|Notebook Index]] | [[Edinburgh/19_August_2008|Next Entry >]]'''
== Week 10 ==
== Week 10 ==
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* Minipreps:
* Minipreps:
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'''M144-M147:''' Minipreps of cenA(1,2,3,4). Mistake made: I used methanol(920ul) instead of ethanol for M144-M147.<br>
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**'''M144-M147:''' Minipreps of ''cenA'' (1,2,3,4). Mistake made: I used methanol(920ul) instead of ethanol for M144-M147.<br>
-
'''M148-M151:''' Minipreps of cex(1,2,3,4).<br>
+
**'''M148-M151:''' Minipreps of ''cex'' (1,2,3,4).<br>
-
'''M152-M155:''' Minipreps of dxs-crtE (1,2,3,4).(OG)
+
**'''M152-M155:''' Minipreps of ''dxs-crtE'' (1,2,3,4).(OG)
* Minipreps 144-155 were digested with EcoRI/PstI. These need running on a gel tomorrow. (AH)  
* Minipreps 144-155 were digested with EcoRI/PstI. These need running on a gel tomorrow. (AH)  
* Maxipreps:
* Maxipreps:
-
'''X11:''' Zea mays isoamylase 1 (isa1+pZntr22).<br>
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**'''X11:''' Zea mays isoamylase 1 (''SU1''+P''zntr22'').<br>
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'''X12:''' Maxiprep of Zea mays isoamylase 2 (isa2+pZntr22). (HX)  
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**'''X12:''' Maxiprep of Zea mays isoamylase 2 (''ISO2''+P''zntr22''). (HX)  
* Combined biobricks:  
* Combined biobricks:  
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'''L42:''' dxs+lims (M137) to appY (M82).<br>
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**'''L42:''' ''dxs''+''LIMS'' (M137) to ''appY'' (M82).<br>
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'''L43:''' crtB+crtI (M109) to appY (M82).<br>
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**'''L43:''' ''crtB''+''crtI'' (M109) to ''appY'' (M82).<br>
-
'''L44:''' pstA (M130) to lacB. (YAN)  
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**'''L44:''' P''cstA'' (M130) to ''lacZ''. (YAN)
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* Blunt-ended ligations '''L45~L47''' of purified P71A (glgC mut 1+2), P71 and P72 (both glgC mut 1+2+3) to SOB2 ("son of BABEL 2"). (AM)
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* Blunt-ended ligations '''L45~L47''' of purified P71A (''glgC'' mut 1+2), P71 and P72 (both ''glgC'' mut 1+2+3) to SOB2 ("son of BABEL 2"). (AM)
* Prepared competent cells (labelled 'iGEM JM109 18/8 1-35') 1-24 = 100 microlitres each. 26-35 = 200 microlitres each. Stored at -80C. (AH, AM)
* Prepared competent cells (labelled 'iGEM JM109 18/8 1-35') 1-24 = 100 microlitres each. 26-35 = 200 microlitres each. Stored at -80C. (AH, AM)
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:::: '''[[Edinburgh/19_August_2008|Next Entry >]]'''
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'''[[Edinburgh/19_August_2008|Next Entry >]]'''

Latest revision as of 21:39, 29 October 2008

< Previous Entry | Notebook Index | Next Entry >

Week 10

Monday 18 August 08

  • Minipreps:
    • M144-M147: Minipreps of cenA (1,2,3,4). Mistake made: I used methanol(920ul) instead of ethanol for M144-M147.
    • M148-M151: Minipreps of cex (1,2,3,4).
    • M152-M155: Minipreps of dxs-crtE (1,2,3,4).(OG)
  • Minipreps 144-155 were digested with EcoRI/PstI. These need running on a gel tomorrow. (AH)
  • Maxipreps:
    • X11: Zea mays isoamylase 1 (SU1+Pzntr22).
    • X12: Maxiprep of Zea mays isoamylase 2 (ISO2+Pzntr22). (HX)
  • Combined biobricks:
    • L42: dxs+LIMS (M137) to appY (M82).
    • L43: crtB+crtI (M109) to appY (M82).
    • L44: PcstA (M130) to lacZ. (YAN)
  • Blunt-ended ligations L45~L47 of purified P71A (glgC mut 1+2), P71 and P72 (both glgC mut 1+2+3) to SOB2 ("son of BABEL 2"). (AM)
  • Prepared competent cells (labelled 'iGEM JM109 18/8 1-35') 1-24 = 100 microlitres each. 26-35 = 200 microlitres each. Stored at -80C. (AH, AM)

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