Team:Chiba/Calendar-Home/10 September 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Communication)
(→Team:Communication)
Line 11: Line 11:
#LuxI+LVA([http://partsregistry.org/Part:BBa_K084014 BBa_K084014])
#LuxI+LVA([http://partsregistry.org/Part:BBa_K084014 BBa_K084014])
#LasI([http://partsregistry.org/Part:BBa_K084007 BBa_K084007])
#LasI([http://partsregistry.org/Part:BBa_K084007 BBa_K084007])
-
#Background(R0010)
+
#Background([http://partsregistry.org/Part:BBa_R0010 R0010])
-
#Background(C0261)
+
#Background([http://partsregistry.org/Part:BBa_C0261 BBa_C0261])
Pick colony
Pick colony

Revision as of 22:12, 29 October 2008

>Home | Notebook

9 September 2008 <|> 11 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(9/9)-->Colony Count

  1. LuxI([http://partsregistry.org/Part:BBa_K084012 BBa_K084012])
  2. LuxI+LVA([http://partsregistry.org/Part:BBa_K084014 BBa_K084014])
  3. LasI([http://partsregistry.org/Part:BBa_K084007 BBa_K084007])
  4. Background([http://partsregistry.org/Part:BBa_R0010 R0010])
  5. Background([http://partsregistry.org/Part:BBa_C0261 BBa_C0261])

Pick colony

Colony-PCR

Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate([http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)).
DNA Template(μL) 1
dNTP mix(μL) 5
Foward Primer(μL) 0.3
Reverse Primer(μL) 0.3
taq DNA polymerase(μL) 0.5
Thermopol Buffer(μL) 3
Nuclease free water(μL) 20
TOTAL(μL) 30


95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C


-->Gel Check

--->(28/8)Miniprep

eluted with 50μL of TE buffer.

Team:Output

TIME Responce(liquid)

TIME Responce(solid)

Colony PCR

-->Gel check

-->Mini prep

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/10_September_2008"