Team:Valencia/Notebook/October

From 2008.igem.org

(Difference between revisions)
(OCtober 27th)
 
(12 intermediate revisions not shown)
Line 121: Line 121:
| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
| Mo || Tu || We || Th || Fr || Sa || <Font Color="#FF0000">Su</Font>
|-
|-
-
| <Font Color="#c0c0c0">29</Font> || <Font Color="#c0c0c0">30</Font> || &nbsp;&nbsp;[[Team:Valencia/Notebook/October#October 1st | 1]]1|| &nbsp;2 || &nbsp;3 || &nbsp;4 || &nbsp;5
+
| <Font Color="#c0c0c0">29</Font> || <Font Color="#c0c0c0">30</Font> || &nbsp;&nbsp;[[Team:Valencia/Notebook/October#October 1st | 1]]|| &nbsp;[[Team:Valencia/Notebook/October#October 2nd | 2]] || &nbsp;3 || &nbsp;4 || &nbsp;5
|-
|-
-
| &nbsp;6 || &nbsp;7 || &nbsp;&nbsp;8|| 9 || 10 || 11|| 12
+
| &nbsp;6 || &nbsp;[[Team:Valencia/Notebook/October#October 7th |7]]  || &nbsp;&nbsp;8|| 9 || 10 || 11|| 12
|-
|-
-
| 13 || 14 || &nbsp;15 || 16 || 17 || 18 || 19
+
| [[Team:Valencia/Notebook/October#October 13th |13]] || [[Team:Valencia/Notebook/October#October 14th |14]]  || &nbsp;15 || 16 || [[Team:Valencia/Notebook/October#October 17th |17]] || 18 || 19
|-
|-
-
| 20 || 21 || &nbsp;22 || 23 || 24 || 25 || 26  
+
| [[Team:Valencia/Notebook/October#October 20th |20]] || 21 || &nbsp;22 || [[Team:Valencia/Notebook/October#October 23rd |23]]  || [[Team:Valencia/Notebook/October#October 24th |24]]  || 25 || 26  
|-
|-
-
| 27 || 28 || &nbsp;29 || 30 || 31 || <Font Color="#c0c0c0">&nbsp;1</Font> || <Font Color="#c0c0c0">&nbsp;2</Font>
+
| [[Team:Valencia/Notebook/October#October 27th |27]]  || 28 || &nbsp;29 || 30 || 31 || <Font Color="#c0c0c0">&nbsp;1</Font> || <Font Color="#c0c0c0">&nbsp;2</Font>
|}
|}
|| || valign="top" |  
|| || valign="top" |  
Line 161: Line 161:
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;270 rpm. <br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;270 rpm. <br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Initial temperature 28 ºC.<br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Initial temperature 28 ºC.<br>
 +
 +
=== October 2nd ===
 +
- Team meeting: We talked about the biobricks and some experiments left.
 +
 +
=== October 7th ===
 +
- We repeated the previous experiment:<br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Four strains in LCCs with [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1 % Galactose induction at the beginning. Initial O.D. 2 <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;270 rpm. <br>
 +
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Initial temperature 28 ºC.<br>
 +
 +
=== October 13th ===
 +
- Total DNA was extracted from our yeast strains <br>
 +
- UCP-1 was amplified by [[Team:Valencia/Parts#Preparing_inserts|PCR]] using oligonucleotides matching the sequence and bearing the appropriate Biobrick prefix and suffix.
 +
 +
=== October 14th ===
 +
- We performed an [[Team:Valencia/Parts#Preparing_inserts|agarose gel electrophoresis]] in order to check our PCR, the results was: Intense amplicons, of the expected size, (about 1 kb) were produced for UCP-1, 175-deleted and 76 deleted.
 +
 +
=== October 17th ===
 +
- Team meeting: We discussed several things that need to be finished, such as sending DNA, preparing presentation, poster, wiki...
 +
 +
=== October 20th ===
 +
- Preparing vectors, competent cells were transformed with: pSB1AK3 and pUC18 plasmids.
 +
 +
=== October 23rd ===
 +
- Plasmids were extracted (High pure miniprep plasmid isolation kit ROCHE).
 +
 +
- Plasmids were digested with EcoRI and PstI
 +
 +
=== October 24th ===
 +
- [[Team:Valencia/Parts#Ligating_Biobricks_into_plasmids|Ligating Biobricks]] into plasmids:
 +
Inserts were run into agarose gels and DNA bands excised with a clean scalpel. DNA was extracted from agarose blocks (ultra clean gel spin, DNA purification Kit, MO BIO laboratories). T4 Ligase was used to ligate inserts and vectors.
 +
 +
- Competent cells were transformed and resulting colonies (Amp LB) screened with Fw and Rv primers to confirm the presence of inserts.
 +
 +
=== October 27th ===
 +
- pSB1AK3 containing UCP-1, 175-deleted and 76-deleted were sent to the Registry.
 +

Latest revision as of 22:14, 29 October 2008



April
Mo Tu We Th Fr Sa Su
31  1   2  3  4  5  6
 7  8   9 10 11 12 13
14 15  16 17 18 19 20
21 22  23 24 25 26 27
28 29  30  1  2  3  4
May
Mo Tu We Th Fr Sa Su
28 29  30  1  2  3  4
 5  6   7  8  9 10 11
12 13  14 15 16 17 18
19 20  21 22 23 24 25
26 27  28 29 30 31 1
June
Mo Tu We Th Fr Sa Su
26 27  28  29 30 31  1
 2  3   4  5  6  7  8
 9 10  11 12 13 14 15
16 17  18 19 20 21 22
23 24  25 26 27 28 29
30  1   2  3  4  5  6
July
Mo Tu We Th Fr Sa Su
30  1  2  3  4  5  6
 7  8   9 10 11 12 13
14 15  16 17 18 19 20
21 22  23 24 25 26 27
28 29  30 31   1  2  3
August
Mo Tu We Th Fr Sa Su
28 29  30 31  1  2  3
 4  5   6  7  8  9 10
11 12  13 14 15 16 17
18 19  20 21 22 23 24
25 26  27 28 29 30 31
September
Mo Tu We Th Fr Sa Su
 1  2   3  4  5  6  7
 8  9  10 11 12 13 14
15 16  17 18 19 20 21
22 23  24 25 26 27 28
29 30  1 2 3 4 5
October
Mo Tu We Th Fr Sa Su
29 30    1   2  3  4  5
 6  7   8 9 10 11 12
13 14  15 16 17 18 19
20 21  22 23 24 25 26
27 28  29 30 31  1  2
November
Mo Tu We Th Fr Sa Su
27 28  29 30 31  1  2
 3  4   5  6  7  8  9
10 11  12 13 14 15 16
17 18  19 20 21 22 23
24 25  26 27 28 29 30


October 1st

- We repeated the previous experiment:
      Four strains in LCCs with SP medium.
      1 % Galactose induction at the beginning. Initial O.D. 2
      270 rpm.
       Initial temperature 28 ºC.

October 2nd

- Team meeting: We talked about the biobricks and some experiments left.

October 7th

- We repeated the previous experiment:
      Four strains in LCCs with SP medium.
      1 % Galactose induction at the beginning. Initial O.D. 2
      270 rpm.
       Initial temperature 28 ºC.

October 13th

- Total DNA was extracted from our yeast strains
- UCP-1 was amplified by PCR using oligonucleotides matching the sequence and bearing the appropriate Biobrick prefix and suffix.

October 14th

- We performed an agarose gel electrophoresis in order to check our PCR, the results was: Intense amplicons, of the expected size, (about 1 kb) were produced for UCP-1, 175-deleted and 76 deleted.

October 17th

- Team meeting: We discussed several things that need to be finished, such as sending DNA, preparing presentation, poster, wiki...

October 20th

- Preparing vectors, competent cells were transformed with: pSB1AK3 and pUC18 plasmids.

October 23rd

- Plasmids were extracted (High pure miniprep plasmid isolation kit ROCHE).

- Plasmids were digested with EcoRI and PstI

October 24th

- Ligating Biobricks into plasmids: Inserts were run into agarose gels and DNA bands excised with a clean scalpel. DNA was extracted from agarose blocks (ultra clean gel spin, DNA purification Kit, MO BIO laboratories). T4 Ligase was used to ligate inserts and vectors.

- Competent cells were transformed and resulting colonies (Amp LB) screened with Fw and Rv primers to confirm the presence of inserts.

October 27th

- pSB1AK3 containing UCP-1, 175-deleted and 76-deleted were sent to the Registry.



Back to September    |    October    |    Go to November