Virginia/25 June 2008
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(Difference between revisions)
(New page: Goals: --make small broth of plated part -B1002 -E0840 stil does not grow --Digest, Purify and ligate RFP(I715039) and GFP(E0240) for a 3a assembly with the standard back bone psb1a3 wit...) |
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Goals: | Goals: | ||
- | --make small broth of plated part -B1002 | + | --make small broth of plated part -B1002 |
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+ | *E0840 stil does not grow | ||
--Digest, Purify and ligate RFP(I715039) and GFP(E0240) for a 3a assembly with the standard back bone psb1a3 with ampR | --Digest, Purify and ligate RFP(I715039) and GFP(E0240) for a 3a assembly with the standard back bone psb1a3 with ampR | ||
+ | |||
+ | --and again for promoter and RBS | ||
+ | |||
+ | --and for BP1 gene and terminator | ||
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+ | |||
+ | [[Team:Virginia/Notebook|Notebook]] |
Latest revision as of 15:13, 27 June 2008
Goals:
--make small broth of plated part -B1002
- E0840 stil does not grow
--Digest, Purify and ligate RFP(I715039) and GFP(E0240) for a 3a assembly with the standard back bone psb1a3 with ampR
--and again for promoter and RBS
--and for BP1 gene and terminator