Team:Chiba/Calendar-Home/10 September 2008

From 2008.igem.org

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===Team:Communication===
===Team:Communication===
-
(9/9)-->
+
(9/9)-->'''Colony Count'''
-
:Colony Count
+
#LuxI([http://partsregistry.org/Part:BBa_K084012 BBa_K084012])
-
#LuxI
+
#LuxI+LVA([http://partsregistry.org/Part:BBa_K084014 BBa_K084014])
-
#LuxI with LVA
+
#LasI([http://partsregistry.org/Part:BBa_K084007 BBa_K084007])
-
#Las
+
#Background([http://partsregistry.org/Part:BBa_R0010 R0010])
-
#Background(R0010)
+
#Background([http://partsregistry.org/Part:BBa_C0261 BBa_C0261])
-
#Background(C0261)
+
Pick colony
Pick colony
'''[[Team:Chiba/protocol/PCR|Colony-PCR]]'''
'''[[Team:Chiba/protocol/PCR|Colony-PCR]]'''
-
::Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate(BBa_F2620[http://partsregistry.org/Part:BBa_F2620](2007)).
+
::Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate([http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)).
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
Line 26: Line 25:
</tr>
</tr>
<tr>
<tr>
-
<td>dNTP mix(μL)</td>
+
<td>dNTP mix(&mu;L)</td>
<td>5</td>
<td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Foward Primer(μL)</td>
+
<td>Foward Primer(&mu;L)</td>
<td>0.3</td>
<td>0.3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Reverse Primer(μL)</td>
+
<td>Reverse Primer(&mu;L)</td>
<td>0.3</td>
<td>0.3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>taq DNA polymerase(μL)</td>
+
<td>taq DNA polymerase(&mu;L)</td>
<td>0.5</td>
<td>0.5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Thermopol Buffer(μL)</td>
+
<td>Thermopol Buffer(&mu;L)</td>
<td>3</td>
<td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Nuclease free water(μL)</td>
+
<td>Nuclease free water(&mu;L)</td>
<td>20</td>
<td>20</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL(μL)</td>
+
<td>TOTAL(&mu;L)</td>
<td>30</td>
<td>30</td>
</tr>
</tr>
</table>
</table>
-
??
+
 
-
:95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃
+
:95&deg;C,5min -> ( 95&deg;C,1min -> 52&deg;C,1min -> 72&deg;C,1min )･･･25cycles -> 72&deg;C,10min -> 6&deg;C
-
??
+
 
-->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
-->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 +
{|align="justify"
 +
|[[Image:Chiba-0910.JPG]]
 +
 +
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample DNA</td>
 +
<td>1</td>
 +
</tr>
 +
<tr>
 +
<td>Loading Dye</td>
 +
<td>1</td>
 +
</tr>
 +
<tr>
 +
<td>dH<sub>2</sub>O</td>
 +
<td>4</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>6μL</td>
 +
</tr>
 +
</table>
 +
 +
|
 +
:From left;
 +
::Marker
 +
::#[http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
 +
::#[http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
 +
::#[http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
 +
::#[http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
 +
::#[http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
 +
::#[http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
 +
::#[http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> Bad
 +
::#[http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
 +
::#[http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
 +
::#[http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
 +
::Marker
 +
::#[http://partsregistry.org/Part:BBa_K084014 BBa_K084014] -> OK
 +
::#[http://partsregistry.org/Part:BBa_K084014 BBa_K084014] -> OK
 +
::#[http://partsregistry.org/Part:BBa_K084014 BBa_K084014] -> OK
 +
::#[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] ---control -> OK
 +
::Marker
 +
|}
 +
--->(28/8)'''[[Team:Chiba/protocol/DNA Purification/sigma|Miniprep]]'''
--->(28/8)'''[[Team:Chiba/protocol/DNA Purification/sigma|Miniprep]]'''
-
eluted with 50μL of TE buffer.
+
eluted with 50&mu;L of TE buffer.
===Team:Output===
===Team:Output===

Latest revision as of 23:10, 29 October 2008

>Home | Notebook

9 September 2008 <|> 11 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(9/9)-->Colony Count

  1. LuxI([http://partsregistry.org/Part:BBa_K084012 BBa_K084012])
  2. LuxI+LVA([http://partsregistry.org/Part:BBa_K084014 BBa_K084014])
  3. LasI([http://partsregistry.org/Part:BBa_K084007 BBa_K084007])
  4. Background([http://partsregistry.org/Part:BBa_R0010 R0010])
  5. Background([http://partsregistry.org/Part:BBa_C0261 BBa_C0261])

Pick colony

Colony-PCR

Colony PCR of 10 colonies from ligation plate(9/9-(2)) and 3 colonies from ligation plate(9/9-(3)) one from control plate([http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)).
DNA Template(μL) 1
dNTP mix(μL) 5
Foward Primer(μL) 0.3
Reverse Primer(μL) 0.3
taq DNA polymerase(μL) 0.5
Thermopol Buffer(μL) 3
Nuclease free water(μL) 20
TOTAL(μL) 30


95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C


-->Gel Check

Chiba-0910.JPG
Sample DNA 1
Loading Dye 1
dH2O 4
TOTAL 6μL
From left;
Marker
  1. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
  2. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
  3. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
  4. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
  5. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
  6. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
  7. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> Bad
  8. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
  9. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
  10. [http://partsregistry.org/Part:BBa_K084007 BBa_K084007] -> OK
Marker
  1. [http://partsregistry.org/Part:BBa_K084014 BBa_K084014] -> OK
  2. [http://partsregistry.org/Part:BBa_K084014 BBa_K084014] -> OK
  3. [http://partsregistry.org/Part:BBa_K084014 BBa_K084014] -> OK
  4. [http://partsregistry.org/Part:BBa_F2620 BBa_F2620] ---control -> OK
Marker


--->(28/8)Miniprep

eluted with 50μL of TE buffer.

Team:Output

TIME Responce(liquid)

TIME Responce(solid)

Colony PCR

-->Gel check

-->Mini prep

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/10_September_2008"