Team:Chiba/Calendar-Home/24 September 2008

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<br>Ligation
<br>Ligation
-
<br>Vector:BBa_R0062①
+
<br>Vector:[http://partsregistry.org/Part:BBa_R0062 R0062]:1
-
<br>Insert:BBa_E0240②
+
<br>Insert:[http://partsregistry.org/Part:BBa_E0240 E0240]:2
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
-
<td>①&②</td><td>①</td>
+
<td>1&2</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(vector)</td>
+
<td>DNA(vector)(&mu;L)</td>
<td>1</td><td>1</td>
<td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(insert)</td>
+
<td>DNA(insert)(&mu;L)</td>
<td>3.2</td><td>-</td>
<td>3.2</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Ligase</td>
+
<td>Ligase(&mu;L)</td>
<td>1</td><td>1</td>
<td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Ligase Buffer</td>
+
<td>Ligase Buffer(&mu;L)</td>
<td>2</td><td>2</td>
<td>2</td><td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>2.5</td><td>6</td>
<td>2.5</td><td>6</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>10</td><td>10</td>
<td>10</td><td>10</td>
</tr>
</tr>
</table>
</table>
-
<br>->TRANSFORMATION
+
<br>->[[Team:Chiba/protocol/transformation|TRANSFORMATION]]

Latest revision as of 23:13, 29 October 2008

>Home | Notebook

23 September 2008 <|> 25 September 2008

Laboratory work

Team:Communication

Team:Output


Ligation
Vector:[http://partsregistry.org/Part:BBa_R0062 R0062]:1
Insert:[http://partsregistry.org/Part:BBa_E0240 E0240]:2

Sample No. 1&21
DNA(vector)(μL) 11
DNA(insert)(μL) 3.2-
Ligase(μL) 11
Ligase Buffer(μL) 22
dH2O(μL) 2.56
TOTAL(μL) 1010


->TRANSFORMATION

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