Team:Chiba/Calendar-Home/13 September 2008

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(Difference between revisions)

Latest revision as of 23:52, 29 October 2008

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12 September 2008 <|> 14 September 2008

Laboratory work

Team:Input

Time Delay Test

time-delay test
- transformants

receiver

[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908)

sender

Plac-RhlI (JW1908)-Amp
Plac-LasI (JW1908)-Amp
Ptet-LuxI (JW1908)-Amp

48-well plate(Deep Well)

1:2ml	2:2ml,AHL	2:2ml	3:2ml	4:2ml	-
-	-	-	-	-	-
1:1ml,2:1ml,IPTG:2μl	1:100μl,2:1ml,IPTG:1.1μl	110μl,21ml,IPTG:101μl	1:1μl,21ml,IPTG:1.001μl	1:1ml,2:100μl,IPTG:1.1μl	1:1ml,2:10μl,IPTG:1.01μl
-	-	-	-	-	-
1:1ml,3:1ml,IPTG:2μl	1:100μl,3:1ml,IPTG:1.1μl	1:10μl,3:1ml,IPTG:1.01μl	1:1μl,3:1ml,IPTG	1:1ml,3:100μl,IPTG:1.1μl	1:1ml,3:10μl,IPTG:1.01μl
-	-	-	-	-	-
1:1ml,4:1ml,IPTG:2μl	1:100μl,4:1ml,IPTG:1.1μl	1:10μl,4:1ml,IPTG:1.0μl	1:1μl,4:1ml,IPTG:1.001μl	1:1ml,4:100μl,IPTG:1.0μl	1:1ml,4:10μl,IPTG1.01μl
-	-	-	-	-	-

Team:Communication

Gel Check

[http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)

Sample No.	1~3
Sample DNA(μL)	1
Loading Dye(μL)	1
dH₂O(μL)	4
TOTAL(μL)	6

From left;

[http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)

-->Digestion

[http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)

Sample No.	1	2	3	4
Sample DNA(μL)	5	5	1	1
EcoRI(μL)	0.5	o.5	-	-
SpeI(μL)	0.5	0.5	-	-
Buffer 1(μL)	1	1	-	-
Buffer 3(μL)	-	-	1	-
BSA(μL)	1	1	-	-
dH₂O(μL)	2	2	7.5	-
TOTAL(μL)	10	10	10	1

--->(9/14)[[1]]

Team:Output

no work

@@ Line 5: / Line 5: @@
 ==Laboratory work==
 ===Team:Input===
 Time Delay Test
-①T9002(Ptet) -p15a-Kan(BW)
+*[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]]
+**transformants
+receiver
+#[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908)
+<BR>sender
+#Plac-RhlI (JW1908)-Amp
+#Plac-LasI (JW1908)-Amp
+#Ptet-LuxI (JW1908)-Amp
-②Plac-RhlI -Amp(BW)
-③Plac-LasI -Amp(BW)
+-well plate(Deep Well)
+<table width="300" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<td width="257">1:2ml</td>
+<td>2:2ml,AHL</td><td>2:2ml</td><td>3:2ml</td><td>4:2ml</td><td>-</td>
+<tr>
+<td>-</td>
+<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
+</tr>
+<tr>
+<td>1:1ml,2:1ml,IPTG:2μl</td>
+<td>1:100&mu;l,2:1ml,IPTG:1.1&mu;l</td><td>110&mu;l,21ml,IPTG:101&mu;l</td><td>1:1μl,21ml,IPTG:1.001&mu;l</td><td>1:1ml,2:100&mu;l,IPTG:1.1&mu;l</td><td>1:1ml,2:10&mu;l,IPTG:1.01&mu;l</td>
+</tr>
+<tr>
+<td>-</td>
+<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
+</tr>
+<tr>
+<td>1:1ml,3:1ml,IPTG:2&mu;l</td>
+<td>1:100&mu;l,3:1ml,IPTG:1.1&mu;l</td><td>1:10&mu;l,3:1ml,IPTG:1.01&mu;l</td><td>1:1μl,3:1ml,IPTG</td><td>1:1ml,3:100&mu;l,IPTG:1.1&mu;l</td><td>1:1ml,3:10&mu;l,IPTG:1.01&mu;l</td>
+</tr>
+<tr>
+<td>-</td>
+<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
+</tr>
+<tr>
+<td>1:1ml,4:1ml,IPTG:2&mu;l</td>
+<td>1:100&mu;l,4:1ml,IPTG:1.1&mu;l</td><td>1:10&mu;l,4:1ml,IPTG:1.0&mu;l</td><td>1:1&mu;l,4:1ml,IPTG:1.001&mu;l</td><td>1:1ml,4:100&mu;l,IPTG:1.0&mu;l</td><td>1:1ml,4:10&mu;l,IPTG1.01&mu;l</td>
+</tr>
+<tr>
+<td>-</td>
+<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
+</tr>
+</table>
-④Ptet-LuxI -Amp(BW)
-*プレ培
-①LB-Kan 2ml培養
-②、③LB-Amp -0.2%グルコース 2ml培養
-④LB-Amp　2ml培養
-↓over night
-*本培
-ml遠心管に①の菌液100μlとLB-Kanを20mlを加える。
+===Team:Communication===
+:'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+::#[http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
+::#[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
+::#[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
-ml遠心管に②、③それぞれの菌液10μlとLB-Amp 0.2%グルコースをそれぞれに6mlを加える。
+{|align="justify"
+|[[Image:Chiba-0913.JPG]]
+|
+:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+<tr>
+<td width="257">Sample No.</td>
+<td>1~3</td>
+</tr>
+<tr>
+<td>Sample DNA(&mu;L)</td>
+<td>1</td></tr>
+<tr>
+<td>Loading Dye(&mu;L)</td>
+<td>1</td></tr>
+<tr>
+<td>dH<sub>2</sub>O(&mu;L)</td>
+<td>4</td></tr>
+<tr>
+<td>TOTAL(&mu;L)</td>
+<td>6</td></tr>
+</table>
+:From left;
+::#[http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
+::#[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
+::#[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
-ml遠心管に④のLB-Ampを6mlを加える。
+|}
-OD>2となったらWash
-*①に関して
+-->'''[[Team:Chiba/protocol/digestion|Digestion]]'''
+::#[http://partsregistry.org/Part:BBa_Co161 BBa_C0161](2007)
+::#[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
+::#[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](2007)
-①
-↓冷却遠心(3700rpm,3min)
-上澄みを捨てる
+<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
-LB-Kanを20ml加える。
-*②③④に関して
-②、③、④
-↓冷却遠心(3700rpm,3min)
-上澄みを捨てる。
-LBを2ml加える。
-:::↓冷却遠心(3700rpm,3min)
-上澄みを捨てる。
-LBを2ml加える。
-:::↓冷却遠心(3700rom,3min)
-上澄み捨てる。
-LB-Ampを6ml加える。
-欠Deep Well
-<table width="300" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
-<td width="257">①:2ml</td>
-<td>②:2ml,AHL</td><td>②:2ml</td><td>③:2ml</td><td>④2ml</td><td>-</td>
 <tr>
-<td>-</td>
+<td width="257">Sample No.</td>
-<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
+<td>1</td><td>2</td><td>3</td><td>4</td>
 </tr>
 <tr>
-<td>①:1ml,②:1ml,IPTG:2μl</td>
+<td>Sample DNA(&mu;L)</td>
-<td>①:100μl,②:1ml,IPTG:1.1μl</td><td>①10μl,②1ml,IPTG:101μl</td><td>①:1μl,②1ml,IPTG:1.001μl</td><td>①:1ml,②:100μl,IPTG:1.1μl</td><td>①:1ml,②:10μl,IPTG:1.01μl</td>
+<td>5</td><td>5</td><td>1</td><td>1</td>
 </tr>
 <tr>
-<td>-</td>
+<td>EcoRI(&mu;L)</td>
-<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
+<td>0.5</td><td>o.5</td><td>-</td><td>-</td>
 </tr>
 <tr>
-<td>①:1ml,③:1ml,IPTG:2μl</td>
+<td>SpeI(&mu;L)</td>
-<td>①:100μl,③:1ml,IPTG:1.1μl</td><td>①:10μl,③:1ml,IPTG:1.01μl</td><td>①:1μl,③:1ml,IPTG</td><td>①:1ml,③:100μl,IPTG:1.1μl</td><td>①:1ml,③:10μl,IPTG:1.01μl</td>
+<td>0.5</td><td>0.5</td><td>-</td><td>-</td>
 </tr>
 <tr>
-<td>-</td>
+<td>Buffer 1(&mu;L)</td>
-<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
+<td>1</td><td>1</td><td>-</td><td>-</td>
 </tr>
 <tr>
-<td>①:1ml,④:1ml,IPTG:2μl</td>
+<td>Buffer 3(&mu;L)</td>
-<td>①100μl,④:1ml,IPTG:1.1μl</td><td>①:10μl,④:1ml,IPTG:1.0μl</td><td>①:1μl,④:1ml,IPTG:1.001μl</td><td>①:1ml,④:100μl,IPTG:1.0μl</td><td>①:1ml,④:10μl,IPTG1.01μl</td>
+<td>-</td><td>-</td><td>1</td><td>-</td>
 </tr>
 <tr>
-<td>-</td>
+<td>BSA(&mu;L)</td>
-<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
+<td>1</td><td>1</td><td>-</td><td>-</td>
+</tr>
+<tr>
+<td>dH<sub>2</sub>O(&mu;L)</td>
+<td>2</td><td>2</td><td>7.5</td><td>-</td>
+</tr>
+<tr>
+<td>TOTAL(&mu;L)</td>
+<td>10</td><td>10</td><td>10</td><td>1</td>
 </tr>
 </table>
-===Team:Communication===
+--->(9/14)[[https://2008.igem.org/Team:Chiba/protocol/ligation/dephosphorylation|SAP]]
-no work
 ===Team:Output===
 no work

Team:Chiba/Calendar-Home/13 September 2008

From 2008.igem.org

Latest revision as of 23:52, 29 October 2008

Contents

Laboratory work

Team:Input

Team:Communication

Team:Output