Circuit Testing

Inputs

• Methionine
• Galactose

Outputs

• CFP
• mCherry
• YFP

Viability Tests

In addition to testing the promoters and parts provided to us, we wish to apply our network to tests that involve

Apoptosis test- induce apoptosis externally and then save cells with the limiter device! -this test has important medical relevance, seeing as many tumor phenotypes are characterized by a noted decline or absence of apoptotic activity

-for yeast, extrinsically-signaled apoptosis depends on oxidative stress or salt -involves the following system: Possible target for downregulation could be Bir1p (from BIR1 gene), a homolog of survivin from humans that is thought to stabilize XIAP Another possible target is AIF1, a homolog of the human AIF1 -released from mito.and travels to nucleus -interesting fact about S. cerevisiae is that they are facultative anaerobic yeast and can survive even with complete removal of mitochondria, making them an ideal system for studying PCD (Frolich- Yeast apoptosos)

http://www.nature.com/embor/journal/v6/n11/full/7400514.html