Edinburgh/Notebook/Plates

From 2008.igem.org

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== Plates ==
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'''Plates 1-2:''' transformation with a BioBrick from the Registry stock: failed. (18.06.08)<br />
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<div id="colortab" class="ddcolortabs">
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<ul>
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Project" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
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<div class="ddcolortabsline">&nbsp;</div>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
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'''Plates 1 and 2:''' transformation with a BioBrick from the Registry stock: failed. (18.06.08)<br />
+
'''Plate 3:''' L1 (pSB1A2+''dxs'') transformation, 100μl. (27.06.08)<br />
'''Plate 3:''' L1 (pSB1A2+''dxs'') transformation, 100μl. (27.06.08)<br />
'''Plate 4:''' L2 (pSB1A2+''appY'') transformation, 100μl. (27.06.08) - Failed<br />
'''Plate 4:''' L2 (pSB1A2+''appY'') transformation, 100μl. (27.06.08) - Failed<br />
Line 302: Line 17:
'''Plate 13:''' possible Babel2+''glgC'' transformants. (28.06.08)<br />
'''Plate 13:''' possible Babel2+''glgC'' transformants. (28.06.08)<br />
'''Plate 14:''' more subcultures from plates 5 and 6. (29.06.08)<br />
'''Plate 14:''' more subcultures from plates 5 and 6. (29.06.08)<br />
-
'''Plates 15 and 16:''' retransformation of religated L2. Failed again. (03.07.08)<br />
+
'''Plates 15-16:''' retransformation of religated L2. Failed again. (03.07.08)<br />
'''Plate 17:''' ''Pantoea ananatis'' for ''crt'' PCR reactions. (05.07.08)<br />
'''Plate 17:''' ''Pantoea ananatis'' for ''crt'' PCR reactions. (05.07.08)<br />
-
'''Plates 18 and 19:''' L5 (pSB1A2+''appY'') transformation, 100μl and 900μl. (05.07.08)<br />
+
'''Plates 18-19:''' L5 (pSB1A2+''appY'') transformation, 100μl and 900μl. (05.07.08)<br />
'''Plate 20:''' subculture from plate 19. (06.07.08)<br />
'''Plate 20:''' subculture from plate 19. (06.07.08)<br />
'''Plate 21:''' subcultures from plate 19. (07.07.08)<br />
'''Plate 21:''' subcultures from plate 19. (07.07.08)<br />
-
'''Plates 22 and 23:''' L6 (BABEL2+''glgC''-mut1) transformation. No colonies. (08.07.08)<br />
+
'''Plates 22-23:''' L6 (BABEL2+''glgC''-mut1) transformation. No colonies. (08.07.08)<br />
-
'''Plates 24 and 25:''' L7 (BABEL2+''glgC''-mut2) transformation. (08.07.08)<br />
+
'''Plates 24-25:''' L7 (BABEL2+''glgC''-mut2) transformation. (08.07.08)<br />
-
'''Plates 26 and 27:''' subcultures from plates 24 and 25 (L7). (09.07.08)<br />
+
'''Plates 26-27:''' subcultures from plates 24 and 25 (L7). (09.07.08)<br />
-
'''Plates 28 and 29:''' ''Cellulomonas fimi'' from initial stock. (11.07.08)<br />
+
'''Plates 28-29:''' ''Cellulomonas fimi'' from initial stock. (11.07.08)<br />
-
'''Plates 30 and 31:''' possible pSB1A2+''crtB'' (L9) transformants. (11.07.08)<br />
+
'''Plates 30-31:''' possible pSB1A2+''crtB'' (L9) transformants. (11.07.08)<br />
-
'''Plates 32 and 33:''' possible pSB1A2+''crtI''-mut (L10) transformants. (11.07.08)<br />
+
'''Plates 32-33:''' possible pSB1A2+''crtI''-mut (L10) transformants. (11.07.08)<br />
'''Plate 34:''' possible pSB1A2+rbs+''dxs'' clones from Nimisha's plates. (11.07.08)<br />
'''Plate 34:''' possible pSB1A2+rbs+''dxs'' clones from Nimisha's plates. (11.07.08)<br />
'''Plate 35:''' patches of possible pSB1A2+''crtB'' transformants from Plate 30. (12.07.08)<br />
'''Plate 35:''' patches of possible pSB1A2+''crtB'' transformants from Plate 30. (12.07.08)<br />
'''Plate 36:''' patches of possible pSB1A2+''crtI''-mut transformants from Plates 32 and 33. (12.07.08)<br />
'''Plate 36:''' patches of possible pSB1A2+''crtI''-mut transformants from Plates 32 and 33. (12.07.08)<br />
-
*Plate 37:* subcultuure of _Cellulomonas fimi_ on NA with filter paper (cellulose)
+
'''Plate 37:''' subculture of ''C. fimi'' on NA with filter paper (cellulose). (15.07.08)<br />
-
*Plates 38 and 39:* L15 transformation (rbs+dxs PCR product with Edinbrick1)
+
'''Plates 38-39:''' L15 transformation (pSB1A2+rbs+''dxs''). (15.07.08)<br />
-
*Plates 40 and 41:* L13 transformation (glgC M19 PCR produce to mutate site 1)
+
'''Plates 40-41:''' L13 transformation (BABEL2+''glgC''-mut1,2). (15.07.08)<br />
-
*Plates 42, 44 and 45:* Patches of possible glgC M19 transformants - subcultures from plate 41
+
'''Plates 42, 44 and 45:''' Patches of possible BABEL2+''glgC''-mut1,2 transformants - subcultures from plate 41. (17.07.08)<br />
-
*Plate 43:* Patches of possible dxs+rbs transformants - subculture from plate 38 or 39
+
'''Plate 43:''' Patches of possible pSB1A2+rbs+''dxs'' transformants - subcultures from plate 38 or 39. (17.07.08)<br />
-
*Plates 46~47:* L16 transformation (appY+rbs PCR product with Edinbrick 1) (18/07/08)
+
'''Plates 46-47:''' L16 transformation (rbs+''appY'' with Edinbrick1) (18.07.08) - No growth<br />
-
*Plates 48~50:* _Cellulomonas fimi_ from overnight cultures made from plate 37. (24/07/08)
+
'''Plates 48-50:''' ''Cellulomonas fimi'' from overnight cultures made from plate 37. (24.07.08: AM)<br />
-
*Plates 51/52:* L19 Transforations (rbs\-_dxs_\-Edinbrick) (26/07/08: CF)
+
'''Plates 51-52:''' L19 (pSB1A2+rbs+''dxs'') transformation. (26.07.08: CF) - No growth<br />
-
*Plates 53/54:* L20 Transforations (rbs\-_appY_\-Edinbrick) (26/07/08: CF)
+
'''Plates 53-54:''' L20 (pSB1A2+rbs+''appY'') transformation. (26.07.08: CF) - No growth<br />
-
*Plates 55/56:* L21 Transforations (rbs\-_crtB_\-Edinbrick) (26/07/08: CF)
+
'''Plates 55-56:''' L21 (pSB1A2+rbs+''crtB'') transformation. (26.07.08: CF) - No growth<br />
-
*Plates 57/58:* L22 Transforations (rbs\-_crtI_\-Edinbrick) (26/07/08: CF)
+
'''Plates 57-58:''' L22 (pSB1A2+rbs+''crtI'') transformation. (26.07.08: CF)<br />
-
*Plates 59/60:* L23 Transforations (PzntA-Edinbrick) (26/07/08: CF)
+
'''Plates 59-60:''' L23 (pSB1A2+P''zntA'') transformation. (26.07.08: CF)<br />
-
*Plates 61/62:* L24 Transforations (_crtE_\-pSB1A2 - from BABEL) (26/07/08: CF)
+
'''Plates 61-62:''' L24 (pSB1A2+''crtE'' - from BABEL2) transformation. (26.07.08: CF)<br />
-
*Plates 63/64:* Repeat transformations of L20 (28/07/08: Yan, OG)
+
'''Plates 63-64:''' Repeat transformation of L20 (pSB1A2+rbs+''appY''). (28.07.08: Yan, OG) - No growth<br />
-
*Plates 65/66:* Repeat transformations of L21 (28/07/08: Yan, OG)
+
'''Plates 65-66:''' Repeat transformation of L21 (pSB1A2+rbs+''crtB''). (28.07.08: Yan, OG)<br />
-
*Plates 67/68:* glgc mutant 1&2 transformation (29.07.2008: Yan)
+
'''Plates 67-68:''' L25 (pSB1A2+''glgC''-mut1,2) transformation. (29.07.2008: Yan)<br />
-
*Plates 69/70:* rbs+dxs, 100 and 900 µl (30.07.2008: CF)
+
'''Plates 69-70:''' pSB1A2+rbs+''dxs'' ligation. (30.07.2008: CF)<br />
-
*Plates 71/72:* crtE coding sequence in Babel2, 100 and 900 µl (30.07.2008: CF)
+
'''Plates 71-72:''' ligation of ''crtE'' to BABEL2. (30.07.2008: CF)<br />
-
*Plate 73:* Subs from Plate 67 (30.07.2008: CF)
+
'''Plate 73:''' Subs from plate 67 (pSB1A2+''glgC''-mut1,2) (30.07.2008: CF)<br />
-
*Plate 74:* Subs from Plates 69-72 (30.07.2008: CF)
+
'''Plate 74:''' Subs from plates 69-72 (pSB1A2+rbs+''dxs'' and BABEL2+''crtE'') (30.07.2008: CF)<br />
-
*Plates 75/76:* L26 (Edi-rbs\-_appY_) 100 microlitres/900 microlitres (31.07.2008: HX)
+
'''Plates 75-76:''' L26 (pSB1A2+rbs+''appY'') transformation. (31.07.2008: HX)<br />
-
*Plates 77/78:* L27 (Edi-rbs\-_crtB_) 100 microlitres/900 microlitres (31.07.2008: HX)
+
'''Plates 77-78:''' L27 (pSB1A2+rbs+''crtB'') transformation. (31.07.2008: HX)<br />
-
*Plates 79/80:* L28 (Edi\-_cenA_) 100 microlitres/900 microlitres (31.07.2008: HX)
+
'''Plates 79-80:''' L28 (pSB1A2+''cenA'') transformation' (31.07.2008: HX)<br />
-
*Plates 81/82:* L29 (Edi\-_cex_) 100 microlitres/900 microlitres (31.07.2008: HX)
+
'''Plates 81-82:''' L29 (pSB1A2+''cex'') transformation' (31.07.2008: HX)<br />
-
*Plate 83:* Subs from Plate 76 (L26: Edi-rbs+appY) (01.08.2008: OG)
+
'''Plate 83:''' Subs from Plate 76 (L26: pSB1A2+rbs+''appY'') (01.08.2008: OG)<br />
-
*Plate 84:* Subs from Plate 77 (L27: Edi-rbs+crtB) (01.08.2008: HX)
+
'''Plate 84:''' Subs from Plate 77 (L27: pSB1A2+rbs+''crtB'') (01.08.2008: HX)<br />
-
*Plate 85:* Subs from Plate 80 (L28: Edi+cenA) (01.08.2008: AM)
+
'''Plate 85:''' Subs from Plate 80 (L28: pSB1A2+''cenA'') (01.08.2008: AM)<br />
-
*Plate 86:* Subs from Plates 81/82 (L29: Edi+cex) (01.08.2008: AM)
+
'''Plate 86:''' Subs from Plates 81-82 (L29: pSB1A2+''cex'') (01.08.2008: AM)<br />
-
*Plate 89/90:* L30 (Self-ligation of P15) 100ml/900ml&nbsp;(06.08.2008: HX)
+
'''Plates 89-90:''' L30 (Self-ligation of BABEL2+''glgC''-mut1,2,3) transformation. (06.08.2008: HX)<br />
-
*Plate 91/92:* L31 (Double digestion of P36/P39 by OG) 100ml/900ml (06.08.2008: HX)
+
'''Plates 91-92:''' L31 (''glgC''-mut1,2 to Edinbrick1) transformation. (06.08.2008: HX)<br />
-
*Plate 93:* Subculture from Plate 56 (M67, pSB1A2-rbs\-_crtB_) (05.08.08: CF)
+
'''Plate 93:''' Subculture from Plate 56 (M67, pSB1A2+rbs+''crtB''). (06.08.08: CF)<br />
-
*Plate 94:* Subculture from Plate 89,90 (07.08.08: HX)
+
'''Plate 94:''' Subcultures from Plates 89-90 (BABEL2+''glgC''-mut1,2,3). (07.08.08: HX)<br />
-
*Plate 95:* Subculture from Plate 92 (07.08.08: HX)
+
'''Plate 95:''' Subculture from Plate 92 (pSB1A2+''glgC''-mut1,2). (07.08.08: HX)<br />
-
*Plate 96:* LB, ampicillin plate spread with subcultures from plate 43. (HX, 07.08.08)
+
'''Plate 96:''' LB, ampicillin plate spread with subcultures from plate 43 (pSB1A2+rbs+''dxs''). (07.08.08: HX)<br />
-
*Plate 97:* LB, ampicillin, 2% glucose plate spread with subcultures from plate 43. (HX, 07.08.08)
+
'''Plate 97:''' LB, ampicillin, 2% glucose plate spread with subcultures from plate 43 (pSB1A2+rbs+''dxs''). (07.08.08: HX)<br />
-
*Plates 98/99:* Transformation of L32 (_crtB_/_crtI_) (08.08.08: HX)
+
'''Plates 98-99:''' Transformation of L32 (pSB1A2+''crtB''+''crtI''). (08.08.08: HX)<br />
-
*Plate 100/101:* Repeat transformation of L31 (Double digestion of P36/P39 by OG) (08.08.08: HX)
+
'''Plates 100-101:''' Repeat transformation of L31 (''glgC''-mut1,2 to Edinbrick1) (08.08.08: HX)<br />
-
*Plate 102:* Patches from plate 99 (L32, crtBI) (10.08.08: CF)
+
'''Plate 102:''' Patches from plate 99 (L32, pSB1A2+''crtB''+''crtI''). (09.08.08: CF)<br />
-
*Plate 103:* Patches from plate 101 (L31, glgC double mutant+pSB1A2) (10.08.08: CF)
+
'''Plate 103:''' Patches from plate 101 (L31, pSB1A2+''glgC''-mut1,2). (09.08.08: CF)<br />
-
*Plate 104/105:* Transform L36 pSB1A2-crtY 100µl/900µl (10.08.08: CF)
+
'''Plate 104-105:''' Transformation of L36 (pSB1A2+''crtY'') 100µl/900µl. (10.08.08: CF)<br />
-
*Plate 106/107:* Transform L37 pSB1A2-rbs-crtY 100µl/900µl (10.08.08: CF)
+
'''Plate 106-107:''' Transformation of L37 (pSB1A2+rbs+''crtY'') 100µl/900µl. (10.08.08: CF)<br />
-
*Plate 108:* Subcultures from plates 105 and 106 (11.08.08: CF)
+
'''Plate 108:''' Subcultures from plates 105 and 106. (11.08.08: CF)<br />
-
*Plate 109/110:* Transformation of L33 pSB1A2-PcstA 100µl/900µl (11.08.08: Yan)
+
'''Plates 109-110:''' Transformation of L33 (pBS1A2+pCstA) 100µl/900µl (11.08.08: Yan)<br />
-
*Plate 111/112:* Transformation of L34 dxs-crtE 100µl/900µl (11.08.08: Yan)
+
'''Plates 111-112:''' Transformation of L34 (pBS1A2+''dxs''+''crtE'') 100µl/900µl (11.08.08: Yan)<br />
-
*Plate 113/114:* Transformation of L35 dxs-lims 100µl/900µl (11.08.08: Yan)
+
'''Plates 113-114:''' Transformation of L35 (pBS1A2+''dxs''+''LIMS1'') 100µl/900µl (11.08.08: Yan)<br />
-
*Plate 115:* Subs from L33 (Pl. 109/110: pSB1A2-PcstA), L34 (Pl. 111/112: dxs-crtE) and L35 (Pl. 113/114: dxs-lims)
+
'''Plate 115:''' Subs from L33 (Pl. 109/110: pSB1A2-P''cstA''), L34 (Pl. 111/112: dxs-crtE) and L35 (Pl. 113/114: ''dxs-LIMS'')<br />
-
*Plates 116/117:* Transformation of L38 (psb1A2\-_crtE_) (13.08.08: HX)
+
'''Plates 116/117:''' Transformation of L38 (psb1A2-''crtE'') (13.08.08: HX)<br />
-
*Plate 118:* Subs from plate 116 (pSB1A2+crtE 100 microlitres). (14.08.08: AH)
+
'''Plate 118:''' Subs from plate 116 (pSB1A2+''crtE'' 100 microlitres). (14.08.08: AH)<br />
-
*Plate 119/120:* Transformation of L40 (CenA+Edinbrick1) (14.08.08: YAN)
+
'''Plate 119/120:''' Transformation of L40 (''cenA''+Edinbrick1) (14.08.08: YAN)<br />
-
*Plate 121/122:* Transformation of L39 (Cex+Edinbrick1) (14.08.08: YAN)
+
'''Plate 121/122:''' Transformation of L39 (''cex''+Edinbrick1) (14.08.08: YAN)<br />
-
*Plate 123/124:* Repeat to transform with L39 (cex+Edinbrick1)&nbsp; (15.08.08: HX)
+
'''Plate 123/124:''' Repeat to transform with L39 (''cex''+Edinbrick1)&nbsp; (15.08.08: HX)<br />
-
*Plate 125/126:* Transform with L40 (cenA+Edinbrick1) (15.08.08: HX)
+
'''Plate 125/126:''' Transform with L40 (''cenA''+Edinbrick1) (15.08.08: HX)<br />
-
*Plate 127/128:* Transform with L41 (dxs/crtE) (15.08.08: HX)
+
'''Plate 127/128:''' Transform with L41 (''dxs/crtE'') (15.08.08: HX)<br />
-
 
+
'''Plate 129:''' ''cenA'' & ''cex'' white colonies from Plates 120 & 123-126 (16/08: CF)<br />
-
*Plate 129:* CenA & CeX white colonies from Plates 120 & 123-126 (16/08: CF)
+
'''Plate 130:''' ''dxs-crtE'' white colonies from plate 127-128 (16/08: CF)<br />
-
 
+
'''Plate 131/132:''' maize pENTR221 transformation, ''SU1 (ISA1)'' Kan 10 (16/08: CF)<br />
-
*Plate 130:* dxs-CrtE white colonies from plate 127-128 (16/08: CF)
+
'''Plate 133/134:''' maize pENTR221 transformation, ''ISO2 (ISA2)'' Kan 10 (16/08: CF)<br />
-
 
+
'''Plate 135:''' patches(colonies) from plates 131,133 (16/08: CF)<br />
-
*Plate 131/132:* maize pENTR221 transformation, ISA1 Kan 10 (16/08: CF)
+
'''Plate 136/137:''' Transformation with L48 (''dxs+LIMS+appY'') (20.08.08: Yan)<br />
-
 
+
'''Plate 138/139:''' Transformation with L49 (''crtBI+appY'') (20.08.08: YAN)<br />
-
*Plate 133/134:* maize pENTR221 transformation, ISA2 Kan 10 (16/08: CF)
+
'''Plate 140:''' Transformation with Edinbrick1 only as control (20.08.08: YAN)<br />
-
 
+
'''Plates 141~147:''' Transformations of L50~L56, 100ml (21.08.08: AM)<br/>
-
*Plate 135:* patches(colonies) from plates 131,133 (16/08: CF)
+
'''Plates 148~154:''' Transformations of L50~L56, 900ml (21.08.08: AM)<br/>
-
 
+
'''Plate 155:''' Subs from plates 137-139. (21.08.08: AH)<br/>
-
*Plate 136/137:* Transformation with L48 (dxs+Lims+Appy) (20.08.08: Yan)
+
'''Plate 158:''' Subs from plates 141, 148: L50 (SOB2-''glgC'' mut 1+2) (22.08.08: AM)<br/>
-
 
+
'''Plate 159:''' Subs from plates 142, 149: L51 (SOB2-''glgC'' mut 1+2+3) (22.08.08: AM)<br/>
-
*Plate 138/139:* Transformation with L49 (CrtBI+Appy) (20.08.08: YAN)
+
'''Plate 160:''' Subs from plates 143, 150: L52 (SOB2-''glgC'' mut 1+2+3); subs from plate 153: L55 (SOB2-rbs-''glgC'' mut 1+2+3 (22.08.08: AM)<br/>
-
 
+
'''Plate 161:''' Subs from plates 144, 151: L53 (SOB2-rbs-''glgC'' mut 1+2) (22.08.08: AM)<br/>
-
*Plate 140/141:* Transformation with Edinbrick1 only as control (20.08.08: YAN)
+
'''Plate 162:''' Subs from plates 147, 154: L56 (''lacZ''-P''cstA'') WHITE colonies (22.08.08: AM)<br/>
 +
'''Plate 163:''' Subs from plates 154: L56 (''lacZ''-P''cstA'') BLUE colonies (22.08.08: AM)<br/>
 +
'''Plate 164:''' 18 subs from plate 126 (pSB1A2+''cenA'') (28.08.08: AH)<br/>
 +
'''Plate 165:''' pSB1A2-CHU2268 (beta glucosidase, from P90), 100µl (28.08.08: AM)<br/>
 +
'''Plate 166:''' pSB1A2-CHU2268 (beta glucosidase, from P90), 900µl (28.08.08: AM)<br/>
 +
'''Plate 167:''' Transformation of L57 (''appY'' into ''crtBI''),100µl (29.08.08: YAN)<br/>
 +
'''Plate 168:''' Transformation of L57 (''appY'' into ''crtBI''),900µl (29.08.08: YAN)<br/>
 +
'''Plate 169:''' Sub-clone from Plate 167-168 (30.08.09: CF)<br/>
 +
'''Plates 170/171:''' Transformation of L58 (04.09.08: CF)<br/>
 +
'''Plates 172/173:''' Transformation of L59 (04.09.08: CF)<br/>
 +
'''Plates 174/175:''' Transformation of L60 (04.09.08: CF)<br/>
 +
'''Plates 176/177:''' Transformation of L61 (04.09.08: CF)<br/>
 +
'''Plate 178:''' sub-clone from L58, L59 (05.09.08: CF)<br/>
 +
'''Plate 179:''' sub-clone from L60, L61 (05.09.08: CF)<br/>
 +
'''Plates 180/181:''' Transformation of L62 (''dxs+crtE'' into ''crtBI'')  (10.09.08: YAN)<br/>
 +
'''Plates 182/183:''' Transformation of L63 (P90 into Edin1) (10.09.08: YAN)<br/>
 +
'''Plates 184/185:''' Transformation of L64 (''cenA'' into Edin1)  (10.09.08: YAN)<br/>
 +
'''Plates 188/189:''' Repeat transformation with L62 (''dxs+crtE'' into ''crtBI''), 100/900µl (11.09.08: AM)<br/>
 +
'''Plates 190/191:''' Repeat transformation with L63 (pSB1A2-CHU2268), 100/900µl (11.09.08: AM)<br/>
 +
'''Plates 192/193:''' Repeat transformation with L64 (pSB1A2-''cenA''), 100/900µl (11.09.08: AM)<br/>
 +
'''Plate 194:''' Transformation with Edinbrick 1 as control, 900µl (11.09.08: AM)<br/>
 +
'''Plate 195:''' Possible SOB-''glgC'' triple mutant subs from plates 186-7 (12.09.08: CF)<br/>
 +
'''Plate 196:''' Possible ''dxs+crtEB2'' subs from plate 181 (12.09.08: CF)<br/>
 +
'''Plate 197:''' Possible ''cenA+bglX'' subs from plates 183, 185 (12.09.08: CF)<br/>
 +
'''Plate 198:''' Possible ''dxs+crtE'' into ''crtBI'' subs from plates 188, 189 (12.09.08: AM)<br/>
 +
'''Plate 199:''' Possible pSB1A2-CHU2268 subs from plate 191 (12.08.08: AM)<br/>
 +
'''Plate 200/201:''' Transformation of L65 (P''lac''+''dxs+LIMS+appY'') (17.09.08:CF)<br/>
 +
'''Plate 202/203:''' Transformation of L66 (P''cstA''+''lacZ' '') (17.09.08:CF)<br/>
 +
'''Plate 204:''' control.

Latest revision as of 00:25, 30 October 2008

< Back to Notebook

Plates

Plates 1-2: transformation with a BioBrick from the Registry stock: failed. (18.06.08)
Plate 3: L1 (pSB1A2+dxs) transformation, 100μl. (27.06.08)
Plate 4: L2 (pSB1A2+appY) transformation, 100μl. (27.06.08) - Failed
Plate 5: L3 (BABEL1+glgC) transformation, 100μl. (27.06.08)
Plate 6: L4 (BABEL2+glgC) transformation, 100μl. (27.06.08)
Plate 7: L1 (pSB1A2+dxs) transformation, 900μl. (27.06.08)
Plate 8: L2 (pSB1A2+appY) transformation, 900μl. (27.06.08) - Failed
Plate 9: L3 (BABEL1+glgC) transformation, 900μl. (27.06.08)
Plate 10: L4 (BABEL2+glgC) transformation, 900μl. (27.06.08)
Plate 11: possible pSB1A2+dxs transformants. (28.06.08)
Plate 12: possible Babel1+glgC transformants. (28.06.08)
Plate 13: possible Babel2+glgC transformants. (28.06.08)
Plate 14: more subcultures from plates 5 and 6. (29.06.08)
Plates 15-16: retransformation of religated L2. Failed again. (03.07.08)
Plate 17: Pantoea ananatis for crt PCR reactions. (05.07.08)
Plates 18-19: L5 (pSB1A2+appY) transformation, 100μl and 900μl. (05.07.08)
Plate 20: subculture from plate 19. (06.07.08)
Plate 21: subcultures from plate 19. (07.07.08)
Plates 22-23: L6 (BABEL2+glgC-mut1) transformation. No colonies. (08.07.08)
Plates 24-25: L7 (BABEL2+glgC-mut2) transformation. (08.07.08)
Plates 26-27: subcultures from plates 24 and 25 (L7). (09.07.08)
Plates 28-29: Cellulomonas fimi from initial stock. (11.07.08)
Plates 30-31: possible pSB1A2+crtB (L9) transformants. (11.07.08)
Plates 32-33: possible pSB1A2+crtI-mut (L10) transformants. (11.07.08)
Plate 34: possible pSB1A2+rbs+dxs clones from Nimisha's plates. (11.07.08)
Plate 35: patches of possible pSB1A2+crtB transformants from Plate 30. (12.07.08)
Plate 36: patches of possible pSB1A2+crtI-mut transformants from Plates 32 and 33. (12.07.08)
Plate 37: subculture of C. fimi on NA with filter paper (cellulose). (15.07.08)
Plates 38-39: L15 transformation (pSB1A2+rbs+dxs). (15.07.08)
Plates 40-41: L13 transformation (BABEL2+glgC-mut1,2). (15.07.08)
Plates 42, 44 and 45: Patches of possible BABEL2+glgC-mut1,2 transformants - subcultures from plate 41. (17.07.08)
Plate 43: Patches of possible pSB1A2+rbs+dxs transformants - subcultures from plate 38 or 39. (17.07.08)
Plates 46-47: L16 transformation (rbs+appY with Edinbrick1) (18.07.08) - No growth
Plates 48-50: Cellulomonas fimi from overnight cultures made from plate 37. (24.07.08: AM)
Plates 51-52: L19 (pSB1A2+rbs+dxs) transformation. (26.07.08: CF) - No growth
Plates 53-54: L20 (pSB1A2+rbs+appY) transformation. (26.07.08: CF) - No growth
Plates 55-56: L21 (pSB1A2+rbs+crtB) transformation. (26.07.08: CF) - No growth
Plates 57-58: L22 (pSB1A2+rbs+crtI) transformation. (26.07.08: CF)
Plates 59-60: L23 (pSB1A2+PzntA) transformation. (26.07.08: CF)
Plates 61-62: L24 (pSB1A2+crtE - from BABEL2) transformation. (26.07.08: CF)
Plates 63-64: Repeat transformation of L20 (pSB1A2+rbs+appY). (28.07.08: Yan, OG) - No growth
Plates 65-66: Repeat transformation of L21 (pSB1A2+rbs+crtB). (28.07.08: Yan, OG)
Plates 67-68: L25 (pSB1A2+glgC-mut1,2) transformation. (29.07.2008: Yan)
Plates 69-70: pSB1A2+rbs+dxs ligation. (30.07.2008: CF)
Plates 71-72: ligation of crtE to BABEL2. (30.07.2008: CF)
Plate 73: Subs from plate 67 (pSB1A2+glgC-mut1,2) (30.07.2008: CF)
Plate 74: Subs from plates 69-72 (pSB1A2+rbs+dxs and BABEL2+crtE) (30.07.2008: CF)
Plates 75-76: L26 (pSB1A2+rbs+appY) transformation. (31.07.2008: HX)
Plates 77-78: L27 (pSB1A2+rbs+crtB) transformation. (31.07.2008: HX)
Plates 79-80: L28 (pSB1A2+cenA) transformation' (31.07.2008: HX)
Plates 81-82: L29 (pSB1A2+cex) transformation' (31.07.2008: HX)
Plate 83: Subs from Plate 76 (L26: pSB1A2+rbs+appY) (01.08.2008: OG)
Plate 84: Subs from Plate 77 (L27: pSB1A2+rbs+crtB) (01.08.2008: HX)
Plate 85: Subs from Plate 80 (L28: pSB1A2+cenA) (01.08.2008: AM)
Plate 86: Subs from Plates 81-82 (L29: pSB1A2+cex) (01.08.2008: AM)
Plates 89-90: L30 (Self-ligation of BABEL2+glgC-mut1,2,3) transformation. (06.08.2008: HX)
Plates 91-92: L31 (glgC-mut1,2 to Edinbrick1) transformation. (06.08.2008: HX)
Plate 93: Subculture from Plate 56 (M67, pSB1A2+rbs+crtB). (06.08.08: CF)
Plate 94: Subcultures from Plates 89-90 (BABEL2+glgC-mut1,2,3). (07.08.08: HX)
Plate 95: Subculture from Plate 92 (pSB1A2+glgC-mut1,2). (07.08.08: HX)
Plate 96: LB, ampicillin plate spread with subcultures from plate 43 (pSB1A2+rbs+dxs). (07.08.08: HX)
Plate 97: LB, ampicillin, 2% glucose plate spread with subcultures from plate 43 (pSB1A2+rbs+dxs). (07.08.08: HX)
Plates 98-99: Transformation of L32 (pSB1A2+crtB+crtI). (08.08.08: HX)
Plates 100-101: Repeat transformation of L31 (glgC-mut1,2 to Edinbrick1) (08.08.08: HX)
Plate 102: Patches from plate 99 (L32, pSB1A2+crtB+crtI). (09.08.08: CF)
Plate 103: Patches from plate 101 (L31, pSB1A2+glgC-mut1,2). (09.08.08: CF)
Plate 104-105: Transformation of L36 (pSB1A2+crtY) 100µl/900µl. (10.08.08: CF)
Plate 106-107: Transformation of L37 (pSB1A2+rbs+crtY) 100µl/900µl. (10.08.08: CF)
Plate 108: Subcultures from plates 105 and 106. (11.08.08: CF)
Plates 109-110: Transformation of L33 (pBS1A2+pCstA) 100µl/900µl (11.08.08: Yan)
Plates 111-112: Transformation of L34 (pBS1A2+dxs+crtE) 100µl/900µl (11.08.08: Yan)
Plates 113-114: Transformation of L35 (pBS1A2+dxs+LIMS1) 100µl/900µl (11.08.08: Yan)
Plate 115: Subs from L33 (Pl. 109/110: pSB1A2-PcstA), L34 (Pl. 111/112: dxs-crtE) and L35 (Pl. 113/114: dxs-LIMS)
Plates 116/117: Transformation of L38 (psb1A2-crtE) (13.08.08: HX)
Plate 118: Subs from plate 116 (pSB1A2+crtE 100 microlitres). (14.08.08: AH)
Plate 119/120: Transformation of L40 (cenA+Edinbrick1) (14.08.08: YAN)
Plate 121/122: Transformation of L39 (cex+Edinbrick1) (14.08.08: YAN)
Plate 123/124: Repeat to transform with L39 (cex+Edinbrick1)  (15.08.08: HX)
Plate 125/126: Transform with L40 (cenA+Edinbrick1) (15.08.08: HX)
Plate 127/128: Transform with L41 (dxs/crtE) (15.08.08: HX)
Plate 129: cenA & cex white colonies from Plates 120 & 123-126 (16/08: CF)
Plate 130: dxs-crtE white colonies from plate 127-128 (16/08: CF)
Plate 131/132: maize pENTR221 transformation, SU1 (ISA1) Kan 10 (16/08: CF)
Plate 133/134: maize pENTR221 transformation, ISO2 (ISA2) Kan 10 (16/08: CF)
Plate 135: patches(colonies) from plates 131,133 (16/08: CF)
Plate 136/137: Transformation with L48 (dxs+LIMS+appY) (20.08.08: Yan)
Plate 138/139: Transformation with L49 (crtBI+appY) (20.08.08: YAN)
Plate 140: Transformation with Edinbrick1 only as control (20.08.08: YAN)
Plates 141~147: Transformations of L50~L56, 100ml (21.08.08: AM)
Plates 148~154: Transformations of L50~L56, 900ml (21.08.08: AM)
Plate 155: Subs from plates 137-139. (21.08.08: AH)
Plate 158: Subs from plates 141, 148: L50 (SOB2-glgC mut 1+2) (22.08.08: AM)
Plate 159: Subs from plates 142, 149: L51 (SOB2-glgC mut 1+2+3) (22.08.08: AM)
Plate 160: Subs from plates 143, 150: L52 (SOB2-glgC mut 1+2+3); subs from plate 153: L55 (SOB2-rbs-glgC mut 1+2+3 (22.08.08: AM)
Plate 161: Subs from plates 144, 151: L53 (SOB2-rbs-glgC mut 1+2) (22.08.08: AM)
Plate 162: Subs from plates 147, 154: L56 (lacZ-PcstA) WHITE colonies (22.08.08: AM)
Plate 163: Subs from plates 154: L56 (lacZ-PcstA) BLUE colonies (22.08.08: AM)
Plate 164: 18 subs from plate 126 (pSB1A2+cenA) (28.08.08: AH)
Plate 165: pSB1A2-CHU2268 (beta glucosidase, from P90), 100µl (28.08.08: AM)
Plate 166: pSB1A2-CHU2268 (beta glucosidase, from P90), 900µl (28.08.08: AM)
Plate 167: Transformation of L57 (appY into crtBI),100µl (29.08.08: YAN)
Plate 168: Transformation of L57 (appY into crtBI),900µl (29.08.08: YAN)
Plate 169: Sub-clone from Plate 167-168 (30.08.09: CF)
Plates 170/171: Transformation of L58 (04.09.08: CF)
Plates 172/173: Transformation of L59 (04.09.08: CF)
Plates 174/175: Transformation of L60 (04.09.08: CF)
Plates 176/177: Transformation of L61 (04.09.08: CF)
Plate 178: sub-clone from L58, L59 (05.09.08: CF)
Plate 179: sub-clone from L60, L61 (05.09.08: CF)
Plates 180/181: Transformation of L62 (dxs+crtE into crtBI) (10.09.08: YAN)
Plates 182/183: Transformation of L63 (P90 into Edin1) (10.09.08: YAN)
Plates 184/185: Transformation of L64 (cenA into Edin1) (10.09.08: YAN)
Plates 188/189: Repeat transformation with L62 (dxs+crtE into crtBI), 100/900µl (11.09.08: AM)
Plates 190/191: Repeat transformation with L63 (pSB1A2-CHU2268), 100/900µl (11.09.08: AM)
Plates 192/193: Repeat transformation with L64 (pSB1A2-cenA), 100/900µl (11.09.08: AM)
Plate 194: Transformation with Edinbrick 1 as control, 900µl (11.09.08: AM)
Plate 195: Possible SOB-glgC triple mutant subs from plates 186-7 (12.09.08: CF)
Plate 196: Possible dxs+crtEB2 subs from plate 181 (12.09.08: CF)
Plate 197: Possible cenA+bglX subs from plates 183, 185 (12.09.08: CF)
Plate 198: Possible dxs+crtE into crtBI subs from plates 188, 189 (12.09.08: AM)
Plate 199: Possible pSB1A2-CHU2268 subs from plate 191 (12.08.08: AM)
Plate 200/201: Transformation of L65 (Plac+dxs+LIMS+appY) (17.09.08:CF)
Plate 202/203: Transformation of L66 (PcstA+lacZ' ) (17.09.08:CF)
Plate 204: control.

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