Team:Chiba/Calendar-Home/18 October 2008

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(New page: 朝senderをwashして菌液10mlとLB-agar10mlを混ぜて固まらないうちにプレートに流し込む。 receiverをニトロセルロースで写し取り、senderのプレー...)
 
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朝senderをwashして菌液10mlとLB-agar10mlを混ぜて固まらないうちにプレートに流し込む。
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>[[Team:Chiba|Home]] | [[Team:Chiba/Notebook|Notebook]]
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[[Team:Chiba/Calendar-Home/17 October 2008|17 October 2008 <]]|[[Team:Chiba/Calendar-Home/19 October 2008|> 19 October 2008]]
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receiverをニトロセルロースで写し取り、senderのプレートにはる。そこをスタートとして37℃の培養機にいれ、30分ごとにUVを当ててGFPが出ているかどうかを確認する。
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==Laboratory work==
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===Team:Demo-Rs===
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17 October~
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#Sender Wash
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##Centrifuged 2 tubes containing([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))at 20&deg;C,3600rpm for 6min and discarded supernatant.
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##Added 10mL LB-Amp to each tube.
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##Repeated wash twice.
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#Creating bacterial plates
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##LB-Amp pre-cultured Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908)) tube 1 (10mL) was mixed with LB-Amp-agar(50&deg;C)(10ml)to produce sender containing bacterialplate-1.
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#Lifted with nitrocellulose
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##Receiver([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))colony was transfered to a nitrocellulose filter and placed on each of Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908))containing bacterial plate (1~3) and Sender-absent negative control plate (t=0). Determined the time required for the colonies to fluoresce depending on the bacterial concentration (100 and 1000-fold dilution).
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#Method to detect fluorescence
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##Plates cultured at 37&deg;C were exposed to UV (312nm) light once every 30 minutes to observe GFP fluorescence.
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==results==
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[[Image:Team-Chiba-P1000911.JPG|150px]]
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[[Image:Team-Chiba-P1000915.JPG|150px]]
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[[Image:Team-Chiba-P1000923.JPG|145px]]
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[[Image:Team-Chiba-P1000931.JPG|143px]]
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[[Image:Team-Chiba-P1000936.JPG|140px]]
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Latest revision as of 00:40, 30 October 2008

>Home | Notebook

17 October 2008 <|> 19 October 2008

Laboratory work

Team:Demo-Rs

17 October~

  1. Sender Wash
    1. Centrifuged 2 tubes containing([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))at 20°C,3600rpm for 6min and discarded supernatant.
    2. Added 10mL LB-Amp to each tube.
    3. Repeated wash twice.
  2. Creating bacterial plates
    1. LB-Amp pre-cultured Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908)) tube 1 (10mL) was mixed with LB-Amp-agar(50°C)(10ml)to produce sender containing bacterialplate-1.
  3. Lifted with nitrocellulose
    1. Receiver([http://partsregistry.org/Part:BBa_T9002 BBa_T9002](JW1908))colony was transfered to a nitrocellulose filter and placed on each of Sender([http://partsregistry.org/Part:BBa_S03623 BBa_S03623](JW1908))containing bacterial plate (1~3) and Sender-absent negative control plate (t=0). Determined the time required for the colonies to fluoresce depending on the bacterial concentration (100 and 1000-fold dilution).
  4. Method to detect fluorescence
    1. Plates cultured at 37°C were exposed to UV (312nm) light once every 30 minutes to observe GFP fluorescence.

results

Team-Chiba-P1000911.JPG Team-Chiba-P1000915.JPG Team-Chiba-P1000923.JPG Team-Chiba-P1000931.JPG Team-Chiba-P1000936.JPG 

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