Latest revision as of 01:14, 30 October 2008

Birds Eye View Team	Projects	Events	Resources
Sponsors	Experiments	Milestones	Protocols
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Things we did today

Grace

Grace

Grace

**Restreak of transformants**
Plate	Colony	Growth?	Blue?
LB+amp₁₀₀	sp_2.5 blue	No	No
	sp₁₀₀ #1	No	No
	sp₁₀₀ #2	No	No
LB	sp_2.5 blue	Yes	Yes, ~20
	sp₁₀₀ #1	Yes	No
	sp₁₀₀ #2	Yes	No
LB+sp₁₀₀	sp_2.5 blue	No	No
	sp₁₀₀ #1	Yes	No
	sp₁₀₀ #2	Yes	No

Restreak plate of colonies 1, 2 and 3 of the lac+rbs_slr+gfpf construct.

Krystle

Viewed restreaks of lac+rbs+slr+gfgf and nir+rbs+slr+gfpf under blue flourescent light.
lac+rbs+slr+gfpf colony 1 glows!

Krystle

Sequencing for lac+rbs+slr+gfpf only had a good reverse read, must be resequenced
All sequenced nir+rbs+slr+gfpf colonies contain the incorrect insert

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 67: / Line 67: @@
-:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
+:* Observations collected 19 hours after initial plating.
-:* e.g. read through papers, worked on proposal, etc.
+[[Image:glowcolony.jpg|right|thumb|Restreak plate of colonies 1, 2 and 3 of the lac+rbs_slr+gfpf construct.]]
+===Verification of GFP secretion device===
+:<strong>Krystle</strong>
+:*Viewed restreaks of lac+rbs+slr+gfgf and nir+rbs+slr+gfpf under blue flourescent light.
+:*lac+rbs+slr+gfpf colony 1 glows!
+==Dry Lab Work==
+=== Analyzed sequencing results===
+:<strong>Krystle </strong>
+:*Sequencing for lac+rbs+slr+gfpf only had a good reverse read, must be resequenced
+:*All sequenced nir+rbs+slr+gfpf colonies contain the incorrect insert
 = Discussion =