Team:Chiba/Reference

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{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
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!align="center"|[[Team:Chiba|Home]]
!align="center"|[[Team:Chiba|Home]]
!align="center"|[[Team:Chiba/Team|The Team]]
!align="center"|[[Team:Chiba/Team|The Team]]
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!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
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__NOTOC__
==UV response==
==UV response==
#Julianne Forman ''et al.:''Photobiological and thermal effects of photoactivating UVA light doses on cell cultures.Photochem. Photobiol. Sci., 2007, 6, 649–658,'''649'''
#Julianne Forman ''et al.:''Photobiological and thermal effects of photoactivating UVA light doses on cell cultures.Photochem. Photobiol. Sci., 2007, 6, 649–658,'''649'''
Line 24: Line 23:
#[http://www.nature.com/nature/journal/v417/n6892/abs/nature00833.html Rong-guang Zhang ''et al''.:Structure of a bacterial quorumsensing transcription factor complexed with pheromone and DNA.NATURE '''417''',2002,971-974]
#[http://www.nature.com/nature/journal/v417/n6892/abs/nature00833.html Rong-guang Zhang ''et al''.:Structure of a bacterial quorumsensing transcription factor complexed with pheromone and DNA.NATURE '''417''',2002,971-974]
#[http://www.sciencemag.org/cgi/content/abstract/sci;272/5268/1655 Margret I. Moré ''et al.:''Enzymatic Synthesis of a Quorum-Sensing Autoinducer Through Use of Defined Substrates.PNAS.1996.272.1655-1658.]
#[http://www.sciencemag.org/cgi/content/abstract/sci;272/5268/1655 Margret I. Moré ''et al.:''Enzymatic Synthesis of a Quorum-Sensing Autoinducer Through Use of Defined Substrates.PNAS.1996.272.1655-1658.]
-
#[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=38458 A. L. SCHAEFER ''et al.:''Generation of cell-to-cell signals in quorum sensing:Acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein.PNAS.93, pp. 9507, September 1996,Biochemistry]
 
#[http://www.pnas.org/content/96/8/4360.abstract M. R. PARSEK ''et al.:'' Acyl homoserine-lactone quorum-sensing signal generation.PNAS.96.pp.4363.1999.Biochemistry]
#[http://www.pnas.org/content/96/8/4360.abstract M. R. PARSEK ''et al.:'' Acyl homoserine-lactone quorum-sensing signal generation.PNAS.96.pp.4363.1999.Biochemistry]
#[http://aem.asm.org/cgi/content/abstract/67/2/575 ANDERSEN ''et al.:''gfp-Based N-Acyl Homoserine-Lactone Sensor Systems for Detection of Bacterial Communication.Vol. 67, No. 2.575–585.2001.APPL. ENVIRON. MICROBIOL.]
#[http://aem.asm.org/cgi/content/abstract/67/2/575 ANDERSEN ''et al.:''gfp-Based N-Acyl Homoserine-Lactone Sensor Systems for Detection of Bacterial Communication.Vol. 67, No. 2.575–585.2001.APPL. ENVIRON. MICROBIOL.]
#[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T30-42G0M1Y-7&_user=136872&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=136872&md5=6c39fbc4f537ea2148bed1f3ad3bfa9b L Ravn. ''et al.:''Methods for detecting acylated homoserine lactones produced by Gram-negative bacteria and their application in studies of AHL-production kinetics.Journal of Microbiological Methods.'''44'''(2001).239-251]
#[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T30-42G0M1Y-7&_user=136872&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=136872&md5=6c39fbc4f537ea2148bed1f3ad3bfa9b L Ravn. ''et al.:''Methods for detecting acylated homoserine lactones produced by Gram-negative bacteria and their application in studies of AHL-production kinetics.Journal of Microbiological Methods.'''44'''(2001).239-251]
 +
 +
====Vibrio fischeri====
 +
#[http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=38458 A. L. SCHAEFER ''et al.:''Generation of cell-to-cell signals in quorum sensing:Acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein.PNAS.93, pp. 9507, September 1996,Biochemistry]
#[http://authors.library.caltech.edu/5553/ C. H. Collins.''et al.:''Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones.Mol.Microbiol.2005.'''55'''(3).712–723]
#[http://authors.library.caltech.edu/5553/ C. H. Collins.''et al.:''Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones.Mol.Microbiol.2005.'''55'''(3).712–723]
 +
 +
====Pseudomonas aeruginosa====
 +
#[http://pubs.acs.org/cgi-bin/abstract.cgi/bichaw/2005/44/i08/abs/bi048005m.html Raychaudhuri.A ''et al''.:Chemical Mechanism and Substrate Specificity of RhlI, an Acylhomoserine Lactone Synthase from Pseudomonas aeruginosa.Biochemistry,44(8),2974 -2981,2005]
 +
#[http://www.pnas.org/content/91/1/197.abstract J P Pearson.''et al.:''Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes.PNAS,4,1994,'''91''',No. 1 ,197-201 ]
#[http://www.pnas.org/content/92/20/9427.abstract M K Winson ''et al.:''Multiple N-acyl-L-homoserine lactone signal molecules regulate production of virulence determinants and secondary metabolites in Pseudomonas aeruginosa.PNAS,(1995),'''92'''No.20,9427-9431]
#[http://www.pnas.org/content/92/20/9427.abstract M K Winson ''et al.:''Multiple N-acyl-L-homoserine lactone signal molecules regulate production of virulence determinants and secondary metabolites in Pseudomonas aeruginosa.PNAS,(1995),'''92'''No.20,9427-9431]
 +
====others====
#[http://www.journalarchive.jst.go.jp/english/jnlabstract_en.php?cdjournal=antibiotics1968&cdvol=46&noissue=3&startpage=441 Chhabra, S. R. ''et al.:''AUTOREGULATION OF CARBAPENEM BIOSYNTHESIS IN Erwinia carotovora BY ANALOGUES OF N-(3-OXOHEXANOYL)-L-HOMOSERINE LACTONE,(1993) J. Antibiot. 46, 441-454.]
#[http://www.journalarchive.jst.go.jp/english/jnlabstract_en.php?cdjournal=antibiotics1968&cdvol=46&noissue=3&startpage=441 Chhabra, S. R. ''et al.:''AUTOREGULATION OF CARBAPENEM BIOSYNTHESIS IN Erwinia carotovora BY ANALOGUES OF N-(3-OXOHEXANOYL)-L-HOMOSERINE LACTONE,(1993) J. Antibiot. 46, 441-454.]
-
#[http://www.pnas.org/content/91/1/197.abstract J P Pearson.''et al.:''Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes.PNAS,4,1994,'''91''',No. 1 ,197-201 ]
+
===Cross-Talk===
-
====Cross-Talk====
+
#[http://www3.interscience.wiley.com/journal/118512088/abstract L. Steindler.V.Venturi.:Detection of quorum sensing N-acyl homoserine lactone signal molecules by bacterial biosensors.FEMS Microbiol Lett '''266''' (2007) 1-9L].
#[http://www3.interscience.wiley.com/journal/118512088/abstract L. Steindler.V.Venturi.:Detection of quorum sensing N-acyl homoserine lactone signal molecules by bacterial biosensors.FEMS Microbiol Lett '''266''' (2007) 1-9L].
#[http://www3.interscience.wiley.com/journal/119124142/abstract M.K Winson ''et al.:''Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing.FEMS Microbiology Letters 163 (1998) 185-192]
#[http://www3.interscience.wiley.com/journal/119124142/abstract M.K Winson ''et al.:''Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing.FEMS Microbiology Letters 163 (1998) 185-192]
#[http://jb.asm.org/cgi/content/abstract/176/10/3076 K.M.GRAY ''et al''.Interchangeability and Specificity of Components from the Quorum-Sensing Regulatory Systems of Vibrio fischeri and Pseudomonas aeruginosa.J.BACTERIOL.'''176''',1994,3076-3080]
#[http://jb.asm.org/cgi/content/abstract/176/10/3076 K.M.GRAY ''et al''.Interchangeability and Specificity of Components from the Quorum-Sensing Regulatory Systems of Vibrio fischeri and Pseudomonas aeruginosa.J.BACTERIOL.'''176''',1994,3076-3080]
-
#[http://www.nature.com/msb/journal/v4/n1/full/msb200824.html FK Balagadde ''et al''.:A synthetic Escherichia coli predator–prey ecosystem.Molecular Systems Biology 4:'''187''',2008]#[http://www.pnas.org/content/104/44/17300.abstract K. Brenner ''et al''.:Engineered bidirectional communication mediates a consensus in a microbial biofilm consortium.PNAS.2007.'''104'''.no.44.17300–17304]
+
#[http://www.nature.com/msb/journal/v4/n1/full/msb200824.html FK Balagadde ''et al''.:A synthetic Escherichia coli predator–prey ecosystem.Molecular Systems Biology 4:'''187''',2008]
 +
#[http://www.pnas.org/content/104/44/17300.abstract K. Brenner ''et al''.:Engineered bidirectional communication mediates a consensus in a microbial biofilm consortium.PNAS.2007.'''104'''.no.44.17300–17304]
#[http://www.nature.com/nbt/journal/v24/n6/abs/nbt1209.html C. H. Collins. ''et al''.:Dual selection enhances the signaling specificity of a variant of the quorum-sensing transcriptional activator LuxR.Nature Biotechnology '''24''', 708 - 712 (2006)]
#[http://www.nature.com/nbt/journal/v24/n6/abs/nbt1209.html C. H. Collins. ''et al''.:Dual selection enhances the signaling specificity of a variant of the quorum-sensing transcriptional activator LuxR.Nature Biotechnology '''24''', 708 - 712 (2006)]
#[http://mic.sgmjournals.org/cgi/content/abstract/151/11/3589 B. Koch. ''et al''.:The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors.Microbiology '''151''' (2005),3589-3602]
#[http://mic.sgmjournals.org/cgi/content/abstract/151/11/3589 B. Koch. ''et al''.:The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors.Microbiology '''151''' (2005),3589-3602]
Line 42: Line 48:
=== Positive Feedback Loops(PFLs))===
=== Positive Feedback Loops(PFLs))===
#[http://pubs.acs.org/cgi-bin/abstract.cgi/acbcct/2006/1/i11/abs/cb6004245.html D. J. Sayut ''et al.:''Construction and Engineering of Positive Feedback Loops.ACS Chemical Biology.'''1'''.No.11.(2006)]<br>
#[http://pubs.acs.org/cgi-bin/abstract.cgi/acbcct/2006/1/i11/abs/cb6004245.html D. J. Sayut ''et al.:''Construction and Engineering of Positive Feedback Loops.ACS Chemical Biology.'''1'''.No.11.(2006)]<br>
-
#[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4PRHJ6K-2&_user=136872&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=136872&md5=3ed7ffab9f831d102f5e99353843c080 D. J. Sayut ''et al.:''Noise and kinetics of LuxR positive feedback loops.Biochem. Biophys. Res. Commun.'''363'''(3),2007,667-673]
+
#[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4PRHJ6K-2&_user=136872&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=136872&md5=3ed7ffab9f831d102f5e99353843c080 D. J. Sayut ''et al.:''Noise and kinetics of LuxR positive feedback loops.Biochem. Biophys. Res. Commun.'''363'''(3),2007,667-673.]
 +
=== AiiA ===
 +
#[http://www.jbc.org/cgi/content/full/279/14/13645 Wang et al.:Specificity and Enzyme Kinetics of the Quorum-quenching N-Acyl Homoserine Lactone Lactonase (AHL-lactonase).J. Biol. Chem.279(14),13645-13651,2004.]
 +
 
=== promoter ===
=== promoter ===
#[http://www.nature.com/msb/journal/v3/n1/synopsis/msb4100187.html Cox RS III, Surette MG,Elowitz MB.:Programming gene expression with combinatorial promoters Mol Syst Biol. 2007 Nov 13;3:145.]
#[http://www.nature.com/msb/journal/v3/n1/synopsis/msb4100187.html Cox RS III, Surette MG,Elowitz MB.:Programming gene expression with combinatorial promoters Mol Syst Biol. 2007 Nov 13;3:145.]
Line 49: Line 58:
#[http://aem.asm.org/cgi/content/abstract/64/6/2240 Andersen.''et al.:''New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria.1998,2240-2246,'''64''',No.6 ]
#[http://aem.asm.org/cgi/content/abstract/64/6/2240 Andersen.''et al.:''New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria.1998,2240-2246,'''64''',No.6 ]
#[http://www.nature.com/nbt/journal/v20/n1/full/nbt0102-87.html T Nagai. ''et al.:''A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications.Nature Biotechnology,'''20''',87 - 90,(2002)]
#[http://www.nature.com/nbt/journal/v20/n1/full/nbt0102-87.html T Nagai. ''et al.:''A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications.Nature Biotechnology,'''20''',87 - 90,(2002)]
 +
#[http://www.ncbi.nlm.nih.gov/pubmed/15558047 Shaner ''et al.:''  Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22, 1567-1572 (2004)]
-
 
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{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |
-
{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
+
!align="center"|[[Team:Chiba|Home]]
!align="center"|[[Team:Chiba|Home]]
!align="center"|[[Team:Chiba/Team|The Team]]
!align="center"|[[Team:Chiba/Team|The Team]]
!align="center"|[[Team:Chiba/Project|The Project]]
!align="center"|[[Team:Chiba/Project|The Project]]
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
!align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
 +
!align="center"|[[Team:Chiba/Reference|Reference]]
!align="center"|[[Team:Chiba/Notebook|Notebook]]
!align="center"|[[Team:Chiba/Notebook|Notebook]]
 +
!align="center"|[[Team:Chiba/Acknowledgements|Acknowledgements]]
|}
|}

Latest revision as of 01:21, 30 October 2008

Chiba-U.gif

UV response

  1. Julianne Forman et al.:Photobiological and thermal effects of photoactivating UVA light doses on cell cultures.Photochem. Photobiol. Sci., 2007, 6, 649–658,649
  2. Jaime L. Matta et al.:Environmental UV-A and UV-B Threshold Doses for Apoptosis and Necrosis in Human Fibroblasts.Photochemistry and Photobiology,2005,81,563–568

Bacterial Communication(Quorum Sensing)

general

  1. [http://www.pnas.org/content/100/suppl.2/14549.full Michiko E. Taga. Bonnie L.Bassler.:Chemical communication among bacteria.PNAS.November 25, 2003,100.suppl.2]
  2. [http://arjournals.annualreviews.org/doi/abs/10.1146/annurev.micro.55.1.165?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dncbi.nlm.nih.gov Melissa B. Miller and Bonnie L. Bassler.:QUORUM SENSING IN BACTERIA.annurev.micro.55.1.165.2001]

specifics

  1. [http://www.nature.com/nature/journal/v417/n6892/abs/nature00833.html Rong-guang Zhang et al.:Structure of a bacterial quorumsensing transcription factor complexed with pheromone and DNA.NATURE 417,2002,971-974]
  2. [http://www.sciencemag.org/cgi/content/abstract/sci;272/5268/1655 Margret I. Moré et al.:Enzymatic Synthesis of a Quorum-Sensing Autoinducer Through Use of Defined Substrates.PNAS.1996.272.1655-1658.]
  3. [http://www.pnas.org/content/96/8/4360.abstract M. R. PARSEK et al.: Acyl homoserine-lactone quorum-sensing signal generation.PNAS.96.pp.4363.1999.Biochemistry]
  4. [http://aem.asm.org/cgi/content/abstract/67/2/575 ANDERSEN et al.:gfp-Based N-Acyl Homoserine-Lactone Sensor Systems for Detection of Bacterial Communication.Vol. 67, No. 2.575–585.2001.APPL. ENVIRON. MICROBIOL.]
  5. [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T30-42G0M1Y-7&_user=136872&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=136872&md5=6c39fbc4f537ea2148bed1f3ad3bfa9b L Ravn. et al.:Methods for detecting acylated homoserine lactones produced by Gram-negative bacteria and their application in studies of AHL-production kinetics.Journal of Microbiological Methods.44(2001).239-251]

Vibrio fischeri

  1. [http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=38458 A. L. SCHAEFER et al.:Generation of cell-to-cell signals in quorum sensing:Acyl homoserine lactone synthase activity of a purified Vibrio fischeri LuxI protein.PNAS.93, pp. 9507, September 1996,Biochemistry]
  2. [http://authors.library.caltech.edu/5553/ C. H. Collins.et al.:Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones.Mol.Microbiol.2005.55(3).712–723]

Pseudomonas aeruginosa

  1. [http://pubs.acs.org/cgi-bin/abstract.cgi/bichaw/2005/44/i08/abs/bi048005m.html Raychaudhuri.A et al.:Chemical Mechanism and Substrate Specificity of RhlI, an Acylhomoserine Lactone Synthase from Pseudomonas aeruginosa.Biochemistry,44(8),2974 -2981,2005]
  2. [http://www.pnas.org/content/91/1/197.abstract J P Pearson.et al.:Structure of the autoinducer required for expression of Pseudomonas aeruginosa virulence genes.PNAS,4,1994,91,No. 1 ,197-201 ]
  3. [http://www.pnas.org/content/92/20/9427.abstract M K Winson et al.:Multiple N-acyl-L-homoserine lactone signal molecules regulate production of virulence determinants and secondary metabolites in Pseudomonas aeruginosa.PNAS,(1995),92No.20,9427-9431]

others

  1. [http://www.journalarchive.jst.go.jp/english/jnlabstract_en.php?cdjournal=antibiotics1968&cdvol=46&noissue=3&startpage=441 Chhabra, S. R. et al.:AUTOREGULATION OF CARBAPENEM BIOSYNTHESIS IN Erwinia carotovora BY ANALOGUES OF N-(3-OXOHEXANOYL)-L-HOMOSERINE LACTONE,(1993) J. Antibiot. 46, 441-454.]

Cross-Talk

  1. [http://www3.interscience.wiley.com/journal/118512088/abstract L. Steindler.V.Venturi.:Detection of quorum sensing N-acyl homoserine lactone signal molecules by bacterial biosensors.FEMS Microbiol Lett 266 (2007) 1-9L].
  2. [http://www3.interscience.wiley.com/journal/119124142/abstract M.K Winson et al.:Construction and analysis of luxCDABE-based plasmid sensors for investigating N-acyl homoserine lactone-mediated quorum sensing.FEMS Microbiology Letters 163 (1998) 185-192]
  3. [http://jb.asm.org/cgi/content/abstract/176/10/3076 K.M.GRAY et al.Interchangeability and Specificity of Components from the Quorum-Sensing Regulatory Systems of Vibrio fischeri and Pseudomonas aeruginosa.J.BACTERIOL.176,1994,3076-3080]
  4. [http://www.nature.com/msb/journal/v4/n1/full/msb200824.html FK Balagadde et al.:A synthetic Escherichia coli predator–prey ecosystem.Molecular Systems Biology 4:187,2008]
  5. [http://www.pnas.org/content/104/44/17300.abstract K. Brenner et al.:Engineered bidirectional communication mediates a consensus in a microbial biofilm consortium.PNAS.2007.104.no.44.17300–17304]
  6. [http://www.nature.com/nbt/journal/v24/n6/abs/nbt1209.html C. H. Collins. et al.:Dual selection enhances the signaling specificity of a variant of the quorum-sensing transcriptional activator LuxR.Nature Biotechnology 24, 708 - 712 (2006)]
  7. [http://mic.sgmjournals.org/cgi/content/abstract/151/11/3589 B. Koch. et al.:The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors.Microbiology 151 (2005),3589-3602]

Positive Feedback Loops(PFLs))

  1. [http://pubs.acs.org/cgi-bin/abstract.cgi/acbcct/2006/1/i11/abs/cb6004245.html D. J. Sayut et al.:Construction and Engineering of Positive Feedback Loops.ACS Chemical Biology.1.No.11.(2006)]
  2. [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4PRHJ6K-2&_user=136872&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_version=1&_urlVersion=0&_userid=136872&md5=3ed7ffab9f831d102f5e99353843c080 D. J. Sayut et al.:Noise and kinetics of LuxR positive feedback loops.Biochem. Biophys. Res. Commun.363(3),2007,667-673.]

AiiA

  1. [http://www.jbc.org/cgi/content/full/279/14/13645 Wang et al.:Specificity and Enzyme Kinetics of the Quorum-quenching N-Acyl Homoserine Lactone Lactonase (AHL-lactonase).J. Biol. Chem.279(14),13645-13651,2004.]

promoter

  1. [http://www.nature.com/msb/journal/v3/n1/synopsis/msb4100187.html Cox RS III, Surette MG,Elowitz MB.:Programming gene expression with combinatorial promoters Mol Syst Biol. 2007 Nov 13;3:145.]

Output

  1. [http://aem.asm.org/cgi/content/abstract/64/6/2240 Andersen.et al.:New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria.1998,2240-2246,64,No.6 ]
  2. [http://www.nature.com/nbt/journal/v20/n1/full/nbt0102-87.html T Nagai. et al.:A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications.Nature Biotechnology,20,87 - 90,(2002)]
  3. [http://www.ncbi.nlm.nih.gov/pubmed/15558047 Shaner et al.: Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22, 1567-1572 (2004)]