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Team:Princeton/ListofPlasmidDesigns
From 2008.igem.org
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The highlight code is as follows: | The highlight code is as follows: | ||
| - | style="background:yellow; color:black" | yellow: overlap of primers with the DNA | + | {|align="left" |
| - | style="background:green; color:black" |dark green: restriction site | + | |style="background:yellow; color:black" | yellow: overlap of primers with the DNA |
| - | style="background:magenta; color:black" |pink: "junk" sequence to allow restriction enzymes to cut near the end of a strand. | + | |} |
| - | style="background:cyan; color:black" |light blue: kozak sequence. | + | |
| - | style="background:teal; color:black" |dark blue: stop codon. | + | {|align="left" |
| - | style="background:gray; color:black" |gray: a 2A sequence (the amino acid sequence is cut by the cell) | + | |style="background:green; color:black" |dark green: restriction site |
| - | style="background:red; color:black" |red: either a linker to fuse two proteins (where indicated) or a sequence with a restriction site removed by changing a base pair with the primer. | + | |} |
| + | |||
| + | {|align="left" | ||
| + | |style="background:magenta; color:black" |pink: "junk" sequence to allow restriction enzymes to cut near the end of a strand. | ||
| + | |} | ||
| + | |||
| + | {|align="left" | ||
| + | |style="background:cyan; color:black" |light blue: kozak sequence. | ||
| + | |} | ||
| + | |||
| + | {|align="left" | ||
| + | |style="background:teal; color:black" |dark blue: stop codon. | ||
| + | |} | ||
| + | |||
| + | {|align="left" | ||
| + | |style="background:gray; color:black" |gray: a 2A sequence (the amino acid sequence is cut by the cell) | ||
| + | |} | ||
| + | |||
| + | {|align="left" | ||
| + | |style="background:red; color:black" |red: either a linker to fuse two proteins (where indicated) or a sequence with a restriction site removed by changing a base pair with the primer. | ||
| + | |} | ||
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{|align="center" | {|align="center" | ||
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[https://2008.igem.org/Media:pLV-Ubc-rtTA-2A-LacIKrab-IRES-Puro.pdf pLV-Ubc-rtTA-2A-LacIKrab-IRES-Puro] | [https://2008.igem.org/Media:pLV-Ubc-rtTA-2A-LacIKrab-IRES-Puro.pdf pLV-Ubc-rtTA-2A-LacIKrab-IRES-Puro] | ||
| + | |} | ||
Latest revision as of 03:58, 30 October 2008
PRINCETON IGEM 2008
| Home | Project Overview | Project Details | Experiments | Results | Notebook |
|---|
| Parts Submitted to the Registry | Modeling | The Team | Gallery |
|---|
The highlight code is as follows:
| yellow: overlap of primers with the DNA |
| dark green: restriction site |
| pink: "junk" sequence to allow restriction enzymes to cut near the end of a strand. |
| light blue: kozak sequence. |
| dark blue: stop codon. |
| gray: a 2A sequence (the amino acid sequence is cut by the cell) |
| red: either a linker to fuse two proteins (where indicated) or a sequence with a restriction site removed by changing a base pair with the primer. |
