Team:LCG-UNAM-Mexico/Experiments/Experiments

From 2008.igem.org

(Difference between revisions)
 
(3 intermediate revisions not shown)
Line 14: Line 14:
<!--
<!--
.Estilo1 {color: #5C743D}
.Estilo1 {color: #5C743D}
 +
.style17 {font-size: 14px}
-->
-->
</style>
</style>
Line 98: Line 99:
<img src="https://static.igem.org/mediawiki/2008/9/99/Ribbon435773498.gif" border="0" width="579" height="9" />
<img src="https://static.igem.org/mediawiki/2008/9/99/Ribbon435773498.gif" border="0" width="579" height="9" />
             <tr>
             <tr>
-
           <td class="pageName"><div align="center">Experiments</div>            </td>
+
           <td class="pageName"><div align="center"><br>
 +
            Experiments</div>            </td>
         </tr>
         </tr>
          
          
Line 106: Line 108:
           <br>
           <br>
     </table>
     </table>
-
        <a href="#Devices"><p align="justify" class="calHeader">Devices</p>
+
      <p align="center" class="bodyText"><a href="#Devices">Devices</a> | <a href="#sensing">Sensing Dispositive </a></p>
-
        <a href="https://static.igem.org/mediawiki/2008/a/a0/123_exp.pdf"><img src="https://static.igem.org/mediawiki/2008/6/6a/123_exp.png" width="500" border="0" /></a>
+
      <p align="justify" class="calHeader style17"><a name="Devices"></a>Devices</p>    
-
           <a href="https://static.igem.org/mediawiki/2008/4/40/Rcna_exp.pdf"><img src="https://static.igem.org/mediawiki/2008/2/21/Rcna_exp.png" width="500" border="0" /></a>
+
      <a href="https://static.igem.org/mediawiki/2008/a/a0/123_exp.pdf">
 +
      <div align="center">
 +
        <p><img src="https://static.igem.org/mediawiki/2008/6/6a/123_exp.png" width="500" border="0" />
 +
          <br>
 +
           <br>
 +
            <a href="https://static.igem.org/mediawiki/2008/4/40/Rcna_exp.pdf"><img src="https://static.igem.org/mediawiki/2008/2/21/Rcna_exp.png" width="500" border="0" /></a>
           </p>
           </p>
-
     
+
        </p>
-
         <p align="justify" class="calHeader"><a href="#Sensing">
+
         <p><a href="#top"><img src="https://static.igem.org/mediawiki/2008/c/cd/Boton_back.jpg" alt="bac to top" width="190" height="31"  border="0" /></a> </p>
-
        </a></p>
+
       </div>
-
       <a href="#Sensing"><p><span class="calHeader">Sensing dispositive</span><br>
+
      <p align="justify" class="calHeader"><a href="#Sensing">
-
         </p></a>
+
         </a><img src="https://static.igem.org/mediawiki/2008/9/99/Ribbon435773498.gif" alt="ribbon" width="579" height="9" /></p>
-
    <div align="center">
+
      <p class="calHeader style17"><a name="sensing"></a>Sensing dispositive</p>
-
          <p><span class="calHeader"><a name="Devices"></a>Devices</span></p>
+
      <p align="justify" class="bodyText">As planned, we started measuring at 0.5 OD in LB medium with gold  electrodes (gold was chosen because of its high conductivity). By this  initial approach satisfactory results were obtained the first day of  measurements. However, the second measurement day we detected there was  not a constant behaviour after each measurement replicate. The cause  seemed to be the LB usage. This medium contains yeast extract which may  be full of divalent ions. We expected to solve this problem by using a  buffer as TE pH 8 or  isotonic saline solution, but anyone of them would cause an stress to  the cell that could modify or measurements in an unpredicted way.<br><br>
-
          <p>&nbsp;</p>
+
      At the same time, we detected that one of the electrodes was stained(oxidation was performed), so we changed the electrodes  metal, avoiding at the same time heating of the electrodes or medium  and the noise that this could imply. Instead of gold we used chrome  electrodes recovered by platinum.<br><br>
-
          <p>&nbsp;</p>
+
        The second approach was performing measurements in LB with chrome  electrodes recovered by platinum. In this way obtained results were  more consistent after each measurement.      </p>
-
          <p>&nbsp;</p>
+
      <p align="left">Full sensing dispositive explanation <a href="https://static.igem.org/mediawiki/2008/2/26/ResistivityMeasurmentsDetails.pdf">here</a>.<br>
-
          <p><span class="calHeader"><a name="Sensing"></a>Sensing dispositive</span><br>
+
      </p>
-
            <br>
+
 
-
          </p>
+
<div align="center">
-
          <p>&nbsp;</p>
+
           <p><br>
           <p><br>
-
          </p>
 
-
          <p align="center">
 
-
         
 
-
          <br>
 
             <br>
             <br>
              
              
-
            <img src="https://static.igem.org/mediawiki/2008/9/99/Ribbon435773498.gif" alt="ribbon" width="579" height="9" />         </p>
+
          <img src="https://static.igem.org/mediawiki/2008/9/99/Ribbon435773498.gif" alt="ribbon" width="579" height="9" />
-
           
+
          </p>
           </p>
           </p>
-
          <p align="center">
+
      <p align="center">
-
   <a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments"><img src="https://static.igem.org/mediawiki/2008/c/cd/Boton_back.jpg"  border="0" width="190" height="31" /></a>
+
   <a href="#top"><img src="https://static.igem.org/mediawiki/2008/c/cd/Boton_back.jpg"  border="0" width="190" height="31" /></a>
<a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments/Design"><img src="https://static.igem.org/mediawiki/2008/4/44/Boton_exp2.jpg" border="0" width="190" height="31" /></a>
<a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments/Design"><img src="https://static.igem.org/mediawiki/2008/4/44/Boton_exp2.jpg" border="0" width="190" height="31" /></a>
<a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments/Experiments"> <img src="https://static.igem.org/mediawiki/2008/f/fc/Boton_exp3.jpg" border="0" width="190" height="31" /></a></p>
<a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments/Experiments"> <img src="https://static.igem.org/mediawiki/2008/f/fc/Boton_exp3.jpg" border="0" width="190" height="31" /></a></p>
-
<a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments/Results"><p align="center"><img src="https://static.igem.org/mediawiki/2008/a/ad/Boton_exp4.jpg" border="0" width="190" height="31" /></p>
+
<p align="center"><a href="https://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments/Results"><img src="https://static.igem.org/mediawiki/2008/a/ad/Boton_exp4.jpg" border="0" width="190" height="31" /></a></p>
  </p>
  </p>
       </div>
       </div>

Latest revision as of 04:09, 30 October 2008

LCG-UNAM-Mexico:Experiments

Header image
iGEM 2008 TEAM
line decor
  
line decor

 
 
 
  



Experiments

Devices | Sensing Dispositive

Devices

ribbon

Sensing dispositive

As planned, we started measuring at 0.5 OD in LB medium with gold electrodes (gold was chosen because of its high conductivity). By this initial approach satisfactory results were obtained the first day of measurements. However, the second measurement day we detected there was not a constant behaviour after each measurement replicate. The cause seemed to be the LB usage. This medium contains yeast extract which may be full of divalent ions. We expected to solve this problem by using a buffer as TE pH 8 or isotonic saline solution, but anyone of them would cause an stress to the cell that could modify or measurements in an unpredicted way.

At the same time, we detected that one of the electrodes was stained(oxidation was performed), so we changed the electrodes metal, avoiding at the same time heating of the electrodes or medium and the noise that this could imply. Instead of gold we used chrome electrodes recovered by platinum.

The second approach was performing measurements in LB with chrome electrodes recovered by platinum. In this way obtained results were more consistent after each measurement.

Full sensing dispositive explanation here.

 
             

Retrieved from "http://2008.igem.org/Team:LCG-UNAM-Mexico/Experiments/Experiments"