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| - | <!-- *** What falls between these lines is the Alert Box! You can remove it from your pages once you have read and understood the alert *** -->
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| - | <div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
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| - | <div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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| - | This is a template page. READ THESE INSTRUCTIONS.
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| - | <div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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| - | You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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| - | <div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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| - | You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your Team:Example namespace.
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| - | {|align="justify"|
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| - | == '''University of Edinburgh 2008 iGEM Team''' ==
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| - | === '''Team Members:''' ===
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| - | '''Students:'''
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| - | Andrew Hall, Adler Ma, Antonia Mayer, Omar Gammoh, Wenhong (Tina) Li, Xing (Ariel) He, Zejun Yan.
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| - | '''Main instructors:'''
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| - | Chris French, Alistair Elfick, Hongwu Ma
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| - | |[[Image:logo-edi.jpg|right|frame]]
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| - | ''Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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| - | |[[Image:Team.png|right|frame|Your team picture]]
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| - | <!--- The Mission, Experiments --->
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| - | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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| - | !align="center"|[[Team:Edinburgh|Home]]
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| - | !align="center"|[[Team:Edinburgh/Team|The Team]]
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| - | !align="center"|[[Team:Edinburgh/Project|The Project]]
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| - | !align="center"|[[Team:Edinburgh/Parts|Parts Submitted to the Registry]]
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| - | !align="center"|[[Team:Edinburgh/Modeling|Modeling]]
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| - | !align="center"|[[Team:Edinburgh/Notebook|Notebook]]
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| - | |}
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| - | (''Or you can choose different headings. But you must have a team page, a project page, and a notebook page.'')
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| - | == '''Overall project''' ==
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| - | === Saving the World ===
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| - | === "Cellulose is the most abundant form of fixed carbon, with 100,000,000,000 tons produced in cell walls by plants each year" ===
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| - | (Wilson, 2008) [http://www.blackwell-synergy.com/doi/full/10.1196/annals.1419.026 ]
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| - | ''' Nowadays the world-wide food shortage is becoming more and more important. As we can image, converting cellulose to starch will be one of the most sufficient ways to solve this problem .'''
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| - | == Project summary==
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| - | === Recent Wiki updates ===
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| - | ==== 01 July 2008 ====
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| - | ''' Lysis: An alternative to cellulase secretion page updated. (AH)'''
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| - | Paris '07designed what they call "synthetic multicellular bacteria" (SMB). This involved reproductively capable "germline" cells which were dependent on diaminopimelate (DAP), a metabolite produced by reproductively incapable "somatic" cells. Low concentrations of DAP triggered the transcription of Cre-recombinase (under the influence of DAP-sensitivepromoter), which caused the removal of a gene required for bacterial reproduction by site-specific recombination (SSR), and resulted in the activation of a gene for the production of DAP by them.
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| - | We could adapt this system to cause some cells to produce cellulases and then lyse to release them into the medium. However, a simpler method could by-pass the need for SSR and could be as follows:
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| - | Our cellulase genes could be under the influence of a promoter sensitive to glucose concentration (activated by low [glucose], inhibited by high [glucose]). Low glucose would, therefore, cause the production of cellulases in some cells.
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| - | We could engineer a reporter gene just downstream of a cellulase gene (under the influence of the same promoter), which could act as a transcriptional activator for lysis genes.
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| - | So low glucose concentration would cause some cells to produce cellulases and the lysis gene transcription factor. Thus the cells would lyse, releasing the cellulases into the cellulose growth medium. The cellulases would work ex vivo to break down cellulose into glucose, which could then be taken up by other cells and converted into starch until the glucose is depleted to insufficient levels.
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| - | This seems to be one of those ideas that, if it works, would be very "elegant", an abstract term that biologists seem to love! It might also provide some more interesting modelling opportunities than the very linear metabolic pathways, and we shouldn't need so much data to model this idea. (I still think that secretion of cellulases would be better though - it would be more straightforward, if possible.)
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| - | Next step along this train of thought: Find a promoter that is repressed by glucose!
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| - | === The Experiments ===
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| - | '''Primer Design'''
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| - | '''Our Primers'''
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| - | '''Dr Chris French's OpenWetWare Site - Our one-stop destination for Biobrick protocols'''
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| - | === External Links ===
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| - | '''Dr. Chris French's Wiki'''
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| - | '''Andy's lab book'''
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| - | '''News and Research Articles'''
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| - | == Results ==
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