Team:University of Ottawa/27 June 2008

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(New page: __TOC__ '''Dan''' :'''Ligation''' ::<li> Ligation was performed on the digested 0 1 A B PCR products, new samples were created according to the following legend: :::)
 
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__TOC__
__TOC__
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==Today in the lab==
'''Dan'''
'''Dan'''
:'''Ligation'''
:'''Ligation'''
::<li> Ligation was performed on the digested 0 1 A B PCR products, new samples were created according to the following legend:
::<li> Ligation was performed on the digested 0 1 A B PCR products, new samples were created according to the following legend:
-
:::
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:::{| class="wikitable"
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|-
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| '''Components''' || '''New Name'''
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|-
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| 0B + 0A || 1
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|-
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| 0B + 1A || 2
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|-
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| 1B + 0A || 3
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|-
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| 1B + 1A || 4
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|}
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::<li> Gel indicated that no ligation occured. There are several reasons for this:
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:::1. We only have a 2 bp overhang
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:::2.We are not using enough enzyme
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:::3. We did not use enough buffer
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::<li>Enough buffer was added and enzyme was doubled, left to react over the weekend

Latest revision as of 19:55, 30 July 2008

Untitled Document

 

 

Contents

Today in the lab

Dan

Ligation
  • Ligation was performed on the digested 0 1 A B PCR products, new samples were created according to the following legend:
    • Components New Name
    0B + 0A 1
    0B + 1A 2
    1B + 0A 3
    1B + 1A 4
  • Gel indicated that no ligation occured. There are several reasons for this:
    1. We only have a 2 bp overhang
    2.We are not using enough enzyme
    3. We did not use enough buffer
  • Enough buffer was added and enzyme was doubled, left to react over the weekend
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/27_June_2008"