Team:NTU-Singapore/Notebook/2 July 2008

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(New page: =Wednesday 2 July= *Arrival of E7+Imm Oligo **PCR E7+Imm *Attempted Ligation of Fe-GFP & LacI-Rbs34 **Failed Attempt due to faulty gel ***Loading dye was unable to keep DNA sample settled ...)
 
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=Wednesday 2 July=
=Wednesday 2 July=
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{|border="1" style="background-color:#ffffcc;" cellpadding="20"
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*Arrival of E7+Imm Oligo
*Arrival of E7+Imm Oligo
**PCR E7+Imm
**PCR E7+Imm
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***Samples settled into the gel and started floating out of it.
***Samples settled into the gel and started floating out of it.
*Preliminary Speculation of problem is due to loading dye.
*Preliminary Speculation of problem is due to loading dye.
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===Luchao, Hung===
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**miniprep for pFe,pLacI,GFP and RBS 34
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**Transformation and cloning for [http://partsregistry.org/wiki/index.php?title=Part:BBa_I763004 BBa_I763004 LacI-GFP] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450 LacI-RFP]. (the transformation on Tuesday was uncessful possibly because of the old agar plates).
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Latest revision as of 02:23, 28 October 2008

Wednesday 2 July

  • Arrival of E7+Imm Oligo
    • PCR E7+Imm
  • Attempted Ligation of Fe-GFP & LacI-Rbs34
    • Failed Attempt due to faulty gel
      • Loading dye was unable to keep DNA sample settled at the bottom of the well
      • Samples settled into the gel and started floating out of it.
  • Preliminary Speculation of problem is due to loading dye.

Luchao, Hung

    • miniprep for pFe,pLacI,GFP and RBS 34
    • Transformation and cloning for [http://partsregistry.org/wiki/index.php?title=Part:BBa_I763004 BBa_I763004 LacI-GFP] and [http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450 LacI-RFP]. (the transformation on Tuesday was uncessful possibly because of the old agar plates).