Team:NTU-Singapore/Notebook/4 July 2008

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(New page: =Friday 4 July= *Hung, Darius: **Ligation of E7 into empty plasmid vector extracted from GFP plasmid. ***Gel electrophoresis for the E7 plasmids synthesized by PCR (with 2 blunt ends recog...)
 
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=Friday 4 July=
=Friday 4 July=
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*Hung, Darius:
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===Hung, Darius===
**Ligation of E7 into empty plasmid vector extracted from GFP plasmid.
**Ligation of E7 into empty plasmid vector extracted from GFP plasmid.
***Gel electrophoresis for the E7 plasmids synthesized by PCR (with 2 blunt ends recognized by EcoRI and PstI).
***Gel electrophoresis for the E7 plasmids synthesized by PCR (with 2 blunt ends recognized by EcoRI and PstI).
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***Ligation of empty plasmid (from GFP) and E7 insert.
***Ligation of empty plasmid (from GFP) and E7 insert.
***Transformation and cloning into homemade top10 cells.
***Transformation and cloning into homemade top10 cells.
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**Lu Chao, Hung: transformation and cell cloning of LacI-GFP and LacI-RFP again.
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===Lu Chao, Hung===
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*Lu Chao: inoculation of LacI-RBS cells.
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transformation and cell cloning of LacI-GFP and LacI-RFP again.
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*Choon Kit: documentation of lab protocols.
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===Lu Chao===
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inoculation of LacI-RBS cells.
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===Choon Kit===
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documentation of lab protocols.
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Latest revision as of 02:24, 28 October 2008


Contents

Friday 4 July

Hung, Darius

    • Ligation of E7 into empty plasmid vector extracted from GFP plasmid.
      • Gel electrophoresis for the E7 plasmids synthesized by PCR (with 2 blunt ends recognized by EcoRI and PstI).
      • Gel extraction of E7 bands (around 2kb) to remove all the oligonucleotides.
      • Purification for E7 after extracted.
      • Digestion of GFP by EcoRI and PstI. Incubation for 3 hours.
      • Digestion of purified E7 by EcoRI and PstI. Incubation for 3 hours.
      • Gel electrophoresis and gel extraction for GFP to get the empty plasmid vector (around 2kb).
      • PCR purification for digested E7 to remove all the enzymes, buffers and small nucleotides.
      • Ligation of empty plasmid (from GFP) and E7 insert.
      • Transformation and cloning into homemade top10 cells.

Lu Chao, Hung

transformation and cell cloning of LacI-GFP and LacI-RFP again.

Lu Chao

inoculation of LacI-RBS cells.

Choon Kit

documentation of lab protocols.

Retrieved from "http://2008.igem.org/Team:NTU-Singapore/Notebook/4_July_2008"