"
Minnesota/2 July 2008
From 2008.igem.org
(Difference between revisions)
Emartin9808 (Talk | contribs) |
Emartin9808 (Talk | contribs) |
||
| (4 intermediate revisions not shown) | |||
| Line 9: | Line 9: | ||
|- | |- | ||
| - | | | + | |'''1. Miraculously receive DB3.1 E.Coli cells''' |
|- | |- | ||
| - | | | + | |'''2. Transformation:''' Transform the plasmid base vector containing ccdB gene into DB3.1 E. Coli. The DB3.1 E. Coli strain is resistant to the toxin produced by the ccdB gene, so we transform the base vector into this to amplify the amount of plasmid DNA. |
| + | |- | ||
| + | |[[Image:plasmidprep.JPG|600px||center|thumb|Plasmid prep base vectors]] | ||
| + | |- | ||
| + | |'''3. Plasmid prep:''' Extract the DNA/plasmid with ccdB gene from DB3.1 E. Coli. Will have a large amount of plasmid base vectors containing ccdB gene, and will then double digest to cut out the ccdB gene at it's restriction sites. | ||
|} | |} | ||
Latest revision as of 21:17, 23 July 2008
| Back to Notebook Home | |
| Go to Previous Day (July 1) | Go to Next Day (July 3) |
| 1. Miraculously receive DB3.1 E.Coli cells |
| 2. Transformation: Transform the plasmid base vector containing ccdB gene into DB3.1 E. Coli. The DB3.1 E. Coli strain is resistant to the toxin produced by the ccdB gene, so we transform the base vector into this to amplify the amount of plasmid DNA. |
|
| 3. Plasmid prep: Extract the DNA/plasmid with ccdB gene from DB3.1 E. Coli. Will have a large amount of plasmid base vectors containing ccdB gene, and will then double digest to cut out the ccdB gene at it's restriction sites. |
