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Revision as of 18:36, 9 July 2008 (view source) Aronlau (Talk | contribs) ← Older edit		Latest revision as of 02:10, 29 October 2008 (view source) Aronlau (Talk | contribs)
(17 intermediate revisions not shown)
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	==pBca1256-K112319==		==pBca1256-K112319==
	<pre>		<pre>
-	PCR al0019/al0020 on MG1655    (465bp, EcoRI/BamHI/DpnI)	+	PCR al0019/al0024 on MG1655 (404bp, gp = A)
		+	PCR al0023/al0023 on MG1655 (86bp, gp=B)
		+	-----------------------------------------------
		+	PCR al0019/al0020 on A+B     (465bp, EcoRI/BamHI/DpnI)
	Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)		Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
	Product is pBca1256-K112319		Product is pBca1256-K112319
Line 250:		Line 253:
	==pBjh1601CK-K112322==		==pBjh1601CK-K112322==
		+	<pre>
	PCR JH089F/JH089R on Salmonella L2.    (379 bp, EcoRI/BamHI/DpnI)		PCR JH089F/JH089R on Salmonella L2.    (379 bp, EcoRI/BamHI/DpnI)
	Sub into pBjh1601CK            (EcoRI/BamHI, 3134+910, L)		Sub into pBjh1601CK            (EcoRI/BamHI, 3134+910, L)
Line 258:		Line 262:
	JH089R  Reverse biobricking of {Pdps}		JH089R  Reverse biobricking of {Pdps}
	cgttaGGATCCaatctcatatcctcttgatg		cgttaGGATCCaatctcatatcctcttgatg
		+	</pre>
	==pBCa1601AC-K112323==		==pBCa1601AC-K112323==
		+	<pre>
	PCR ca998/JH090R on pBjh1601AC-K112321-2    (445 bp, EcoRI/BamHI)		PCR ca998/JH090R on pBjh1601AC-K112321-2    (445 bp, EcoRI/BamHI)
	Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)		Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)
Line 268:		Line 274:
	JH090R  Reverse biobricking of {H-NS!} Salmonella		JH090R  Reverse biobricking of {H-NS!} Salmonella
	cgttaGGATCCTTATTcCTTGATCAGG		cgttaGGATCCTTATTcCTTGATCAGG
		+	</pre>
		+	==pBCa1601CK-K112324==
		+	<pre>
		+	Digest (BgIII methylated) K112902 CA, (BamHI methylated) K112709 AK      (BamHI/BgIII/XHoI)
		+	Product is pBjh1601CK-K112324
		+	</pre>
		+
		+	==pBCa1601CK-K112325==
		+	<pre>
		+	Digest (BgIII methylated) K112704 CA, (BamHI methylated) K112709 AK      (BamHI/BgIII/XHoI)
		+	Product is pBjh1601CK-K112325
		+	</pre>
		+
		+	==pBCa1601AC-K112327==
		+	<pre>
		+	Digest (BgIII methylated) K112230 AK, (BamHI methylated) K112203 KC      (BamHI/BgIII/XHoI)
		+	Product is pBjh1601AC-K112327
		+	</pre>
		+
		+	==pBCa1601AC-K112328==
		+	<pre>
		+	Digest (BgIII methylated) K112227 AK, (BamHI methylated) K112102 KC      (BamHI/BgIII/XHoI)
		+	Product is pBjh1601AC-K112328
		+	</pre>
		+
		+	==pBCa1601AC-K112329==
		+	<pre>
		+	Digest  (BgIII methylated) K112230 AK, (BamHI methylated) K112113 CK      (BamHI/BgIII/XHoI)
		+	Product is pBjh1601AC-K112329
		+	</pre>
		+
		+	==pBCa1601AC-K112330==
		+	<pre>
		+	Digest K112614 AK (BgIII, XhoI)
		+	Digest K112105 KC(BamHI, XhoI)
		+	Product is pBjh1601AC-K112330
		+	</pre>
		+
		+	==pBCa1601AC-K112331==
		+	<pre>
		+	Digest K112615 AK (BgIII, XhoI)
		+	Digest K112108 KC(BamHI, XhoI)
		+	Product is pBjh1601AC-K112331
		+	</pre>
		+
		+	==pBCa1601AC-K112332==
		+	<pre>
		+	Digest  (BgIII methylated) K112615 AK, (BamHI methylated) K112216 KC      (BamHI/BgIII/XHoI)
		+	Product is pBjh1601AC-K112332
		+	</pre>
		+
		+	==pBCa1601AC-K112333==
		+	<pre>
		+	Digest (BgIII methylated) K112614 AK, (BamHI methylated) K112222 KC      (BamHI/BgIII/XHoI)
		+	Product is pBjh1601AC-K112333
		+	</pre>
		+
		+	==pBca1601AK-K112334==
		+	<pre>
		+	Digest (BgIII methylated) K112230 AC, (BamHI methylated) K112324 CK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601AK-K112334
		+	</pre>
		+
		+	==pBca1601AK-K112335==
		+	<pre>
		+	Digest (BgIII methylated) K112227 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
		+	Product is pBjh1601AK-K112335
		+	</pre>
		+
		+	==pBca1601AK-K112336==
		+	<pre>
		+	Digest (BgIII methylated) K112614 AC   (BamHI/XhoI)
		+	Digest (BamHI methylated) K112324 CK      (BgIII/XHoI)
		+	Product is pBjh1601AK-K112336
		+	</pre>
		+
		+	==pBca1601AK-K112337==
		+	<pre>
		+	Digest (BgIII methylated) K112615 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
		+	Product is pBjh1601AK-K112337
		+	</pre>
		+
		+	==pBca1601AK-K112339==
		+	<pre>
		+	Digest (BgIII methylated) K112327 AC    (BamHI/XhoI)
		+	Digest (BamHI methylated) K112324 CK    (BgIII/XHoI)
		+	Product is pBjh1601AK-K112339
		+	</pre>
		+
		+
		+	==pBca1601AK-K112340==
		+	<pre>
		+	Digest (BgIII methylated) K112328 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
		+	Product is pBjh1601AK-K112340
		+	</pre>
		+
		+	==pBca1601AK-K112341==
		+	<pre>
		+	Digest (BgIII methylated) K112329 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
		+	Product is pBjh1601AK-K112341
		+	</pre>
		+
		+	==pBca1601AK-K112342==
		+	<pre>
		+	Digest (BgIII methylated) K112330 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
		+	Product is pBjh1601AK-K112342
		+	</pre>
		+
		+
		+	==pBca1601AK-K11234==
		+	<pre>
		+	Digest (BgIII methylated) K112332 AC, (BamHI methylated) K112324 CK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601AK-K112344
		+	</pre>
		+
		+	==pBca1601AK-K112348==
		+	<pre>
		+	Digest (BgIII methylated) K112328 AC, (BamHI methylated) K112325 CK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601AK-K112348
		+	</pre>
		+
		+	==pBca1601AK-K112349==
		+	<pre>
		+	Digest (BgIII methylated) K112329 AC, (BamHI methylated) K112325 CK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601AK-K112349
		+	</pre>
		+
		+	==pBca1601AK-K112350==
		+	<pre>
		+	Digest (BgIII methylated) K112330 AC (BamHI/XhoI)
		+	Digest (BamHI methylated) K112325 CK    (BgIII/XHoI)
		+	Product is pBjh1601AK-K112350
		+	</pre>
		+	==pBca1601AK-K112351==
		+	<pre>
		+	Digest (BgIII methylated) K112331 AC (BamHI/XhoI)
		+	Digest (BamHI methylated) K112325 CK    (BgIII/XHoI)
		+	Product is pBjh1601AK-K112351
		+	</pre>
		+	==pBca1601CK-K112354==
		+	<pre>
		+	Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112334 AK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601CK-K112354
		+	</pre>
		+	==pBca1601CK-K112355==
		+	<pre>
		+	Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112335 AK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601CK-K112354
		+	</pre>
		+	==pBca1601CK-K112356==
		+	<pre>
		+	Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112336 AK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601CK-K112354
		+	</pre>
		+	==pBca1601CK-K112357==
		+	<pre>
		+	Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112337 AK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601CK-K112354
		+	</pre>
		+	==pBca1601CK-K112360==
		+	<pre>
		+	Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112340 AK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601CK-K112360
		+	</pre>
		+	==pBca1601CK-K112361==
		+	<pre>
		+	Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112341 AK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601CK-K112361
		+	</pre>
		+	==pBca1601CK-K112362==
		+	<pre>
		+	Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112342 AK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601CK-K112362
		+	</pre>
		+	==pBca1601CK-K112363==
		+	<pre>
		+	Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112343 AK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601CK-K112363
		+	</pre>
		+	==pBca1601CK-K112368==
		+	<pre>
		+	Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112348 AK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601CK-K112368
		+	</pre>
		+	==pBca1601CK-K112369==
		+	<pre>
		+	Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112349 AK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601CK-K112369
		+	</pre>
		+	==pBca1601CK-K112370==
		+	<pre>
		+	Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112350 AK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601CK-K112370
		+	</pre>
		+	==pBca1601CK-K112371==
		+	<pre>
		+	Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112351 AK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601CK-K112371
		+	</pre>
		+	==pBca1601CK-K112372==
		+	<pre>
		+	Digest (BgIII methylated) K112121 CA, (BamHI methylated) K112709 AK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601CK-K112372
		+	</pre>
		+	==pBca1601CK-K112373==
		+	<pre>
		+	Digest (BgIII methylated) BBa_K112232 CA, (BamHI methylated) K112709 AK    (BamHI/BgIII/XHoI)
		+	Product is pBjh1601CK-K112373
		+	</pre>

---

Latest revision as of 02:10, 29 October 2008

Contents 1 pBca1256-K112300 2 pBca1256-K112301 3 pBca1256-K112302 4 pBca1256-K112303 5 pBca1256-K112304 6 pBca1256-K112305 7 pBca1256-K112306 8 pBca1256-K112307 9 pBca1256-K112308 10 pBca1256-K112309 11 pBca1256-K112310 12 pBca1256-K112311 13 pBca1256-K112312 14 pBca1256-K112313 15 pBca1256-K112314 16 pBca1256-K112315 17 pBca1256-K112316 18 pBca1256-K112317 19 pBca1256-K112318 20 pBca1256-K112319 21 pBca1256-K112320 22 pBjh1601AC-K112321 23 pBjh1601CK-K112322 24 pBCa1601AC-K112323 25 pBCa1601CK-K112324 26 pBCa1601CK-K112325 27 pBCa1601AC-K112327 28 pBCa1601AC-K112328 29 pBCa1601AC-K112329 30 pBCa1601AC-K112330 31 pBCa1601AC-K112331 32 pBCa1601AC-K112332 33 pBCa1601AC-K112333 34 pBca1601AK-K112334 35 pBca1601AK-K112335 36 pBca1601AK-K112336 37 pBca1601AK-K112337 38 pBca1601AK-K112339 39 pBca1601AK-K112340 40 pBca1601AK-K112341 41 pBca1601AK-K112342 42 pBca1601AK-K11234 43 pBca1601AK-K112348 44 pBca1601AK-K112349 45 pBca1601AK-K112350 46 pBca1601AK-K112351 47 pBca1601CK-K112354 48 pBca1601CK-K112355 49 pBca1601CK-K112356 50 pBca1601CK-K112357 51 pBca1601CK-K112360 52 pBca1601CK-K112361 53 pBca1601CK-K112362 54 pBca1601CK-K112363 55 pBca1601CK-K112368 56 pBca1601CK-K112369 57 pBca1601CK-K112370 58 pBca1601CK-K112371 59 pBca1601CK-K112372 60 pBca1601CK-K112373

pBca1256-K112300

PCR al0001/al0002 on pSB3C6-Bjh1501     (508bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112300
-----------------------------------------------
al0001	Forward Biobricking of lambda lysozyme with start and stop codons
CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
al0002	Reverse Biobricking of lambda lysozyme with start and stop codons
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112301

PCR al0003/al0002 on pSB3C6-Bjh1501     (505bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112301
-----------------------------------------------
al0003	Forward Biobricking of Lambda Lysozyme with stop codon and no start codon
CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
al0002	Reverse Biobricking of Lambda Lysozyme with stop codon and no start codon
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112302

PCR al0001/al0004 on pSB3C6-Bjh1501     (505bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112302
-----------------------------------------------
al0001	Forward Biobricking of lambda lysozyme with start codon and no stop codon
CCATAGAATTCatgAGATCTatggtagaaatcaataatcaacgtaaggcgttcctcg
al0004	Reverse Biobricking of lambda lysozyme with start codon and no stop codon
CGttaGGATCCtacatcaatctctctgaccgttccgc

pBca1256-K112303

PCR al0003/al0004 on pSB3C6-Bjh1501     (502bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112303
-----------------------------------------------
al0003	Forward Biobricking of lambda lysozyme with no start/stop codon
CCATAGAATTCatgAGATCTgtagaaatcaataatcaacgtaaggcgttcctcg
al0004	Reverse Biobricking of lambda lysozyme with no start/stop codon
CGttaGGATCCtacatcaatctctctgaccgttccgc

pBca1256-K112304

PCR al0005/al0002 on pSB3C6-Bjh1501     (512bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112304
-----------------------------------------------
al0005	Forward Biobricking of lambda lysozyme with spacer
CCATAGAATTCatgAGATCTgaagatggtagaaatcaataatcaacgtaaggcgttcctcg
al0002	Reverse Biobricking of lambda lysozyme with spacer
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112305

PCR al0006/al0002 on pSB3C6-Bjh1501     (527bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112305
-----------------------------------------------
al0006	Forward Biobricking of lambda lysozyme with rbs site
CCATAGAATTCatgAGATCTaaaaaagccggagtagaagatgg
al0002	Reverse Biobricking of lambda lysozyme with rbs site
CGttaGGATCCtcatacatcaatctctctgaccgttccgcc

pBca1256-K112306

PCR al0007/al0008 on pSB3C6-Bjh1502     (349bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112306
-----------------------------------------------
al0007	Forward Biobricking of lambda holin with start and stop codons
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg
al0008	Reverse Biobricking of lambda holin with start and stop codons
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112307

PCR al0009/al0008 on pSB3C6-Bjh1502     (346bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112307
-----------------------------------------------
al0009	Forward Biobricking of lambda holin with stop codon and no start codon
CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc
al0008	Reverse Biobricking of lambda holin with stop codon and no start codon
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112308

PCR al0007/al0010 on pSB3C6-Bjh1502     (346bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112308
-----------------------------------------------
al0007	Forward Biobricking of lambda holin with start and no stop codon
CCATAGAATTCatgAGATCTatgccagaaaaacatgacctgttgg
al0010	Reverse Biobricking of lambda holin wtih start and no stop codon
CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112309

PCR al0009/al0010 on pSB3C6-Bjh1502     (343bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112309
-----------------------------------------------
al0009	Forward Biobricking of lambda holin with no start and stop codon
CCATAGAATTCatgAGATCTccagaaaaacatgacctgttggcc
al0010	Reverse Biobricking of lambda holin with no start and stop codon
CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112310

PCR al0011/al0008 on pSB3C6-Bjh1502     (353bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112310
-----------------------------------------------
al0011	Forward Biobricking of lambda holin with spacer
CCATAGAATTCatgAGATCTgaagatgccagaaaaacatgacctg
al0008	Reverse Biobricking of lambda holin with spacer
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112311

PCR al0012/al0008 on pSB3C6-Bjh1502     (369bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112311
-----------------------------------------------
al0012	Forward Biobricking of lambda holin with rbs site
CCATAGAATTCatgAGATCTattgggggtaagacCtgaag
al0008	Reverse Biobricking of lambda holin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112312

PCR al0013/al0008 on pSB3C6-Bjh1503     (355bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112312
-----------------------------------------------
al0013	Forward Biobricking of lambda antiholin with start and stop codons
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
al0008	Reverse Biobricking of lambda antiholin with start and stop codons
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112313

PCR al0014/al0008 on pSB3C6-Bjh1503     (352bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112313
-----------------------------------------------
al0014	Forward Biobricking of lambda antiholin with stop codon and no start codon
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
al0008	Reverse Biobricking of lambda antiholin with stop codon and no start codon
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112314

PCR al0013/al0010 on pSB3C6-Bjh1503     (352bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112314
-----------------------------------------------
al0013	Forward Biobricking of lambda antiholin with start codon and no stop codon
CCATAGAATTCatgAGATCTatgaagCtgccagaaaaacatgacc
al0010	Reverse Biobricking of lambda antiholin with start codon and no stop codon
CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112315

PCR al0014/al0010 on pSB3C6-Bjh1503     (349bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112315
-----------------------------------------------
al0014	Forward Biobricking of lambda antiholin with no start and stop codons
CCATAGAATTCatgAGATCTaagCtgccagaaaaacatgacctg
al0010	Reverse Biobricking of lambda antiholin with no start and stop codons
CGttaGGATCCttgatttctaccatcttctactccggc

pBca1256-K112316

PCR al0015/al0008 on pSB3C6-Bjh1503     (359bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112316
-----------------------------------------------
al0015	Forward Biobricking of lambda antiholin with spacer
CCATAGAATTCatgAGATCTagacatgaagCtgccagaaaaacatgacc
al0008	Reverse Biobricking of lambda antiholin with spacer
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112317

PCR al0016/al0008 on pSB3C6-Bjh1503     (375bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112317
-----------------------------------------------
al0016	Forward Biobricking of lambda antiholin with rbs site
CCATAGAATTCatgAGATCTccccttattgggggtaagacatg
al0008	Reverse Biobricking of lambda antiholin with rbs site
CGttaGGATCCttattgatttctaccatcttctactccggc

pBca1256-K112318

PCR al0017/al0018 on MG1655     (467bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112318
-----------------------------------------------
al0017	Forward Biobricking of bolA promoter
CCATAGAATTCatgAGATCTtgctgtggcagtgtaatcgtc
al0018	Reverse Biobricking of bolA promoter
CGttaGGATCCcttctatccgctcacgtatc

pBca1256-K112319

PCR al0019/al0024 on MG1655 (404bp, gp = A)
PCR al0023/al0023 on MG1655 (86bp, gp=B)
-----------------------------------------------
PCR al0019/al0020 on A+B     (465bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112319
-----------------------------------------------
al0019	Forward Biobricking of ftsQ promoter
CCATAGAATTCatgAGATCTttgattgaaaaatggctaagtgggccgg
al0020	Reverse Biobricking of ftsQ promoter
CGttaGGATCCcctcttcttcgctgtttcgc
al0023 Forward Removing of EcoRI
ggtagtacgaattGtggaactggcg
al0024 Reverse Removing of EcoRI
cgccagttccaCaattcgtactacc

pBca1256-K112320

PCR al0021/al0022 on MG1655     (804bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112320
-----------------------------------------------
al0021	Forward Biobricking of ftsAZ promoter
CCATAGAATTCatgAGATCTaacgacagtgttggtgagcg
al0022	Reverse Biobricking of ftsAZ promoter
CGttaGGATCCgtaccaatctccagtcctactaccag

pBjh1601AC-K112321

PCR JH088F/JHO88R on Salmonella L2.     (435bp, EcoRI/BamHI/DpnI)
Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)
Product is pBjh1601AC-K112321
-----------------------------------------------
JH088F   Forward biobricking of {H-NS!}   
cgataGAATTCatgAGATCTATGAGCGAAGCACTTAAAATTC
JH088R   Reverse biobricking of {H-NS!}   
cgttaGGATCCTTATTGCTTGATCAGGAAATCG

pBjh1601CK-K112322

PCR JH089F/JH089R on Salmonella L2.    (379 bp, EcoRI/BamHI/DpnI)
Sub into pBjh1601CK             (EcoRI/BamHI, 3134+910, L)
Product is pBjh1601CK-K112322
-----------------------------------------------
JH089F   Forward biobricking of {Pdps}   
cgataGAATTCatgAGATCTaacgattcgacgcgaaccgc
JH089R   Reverse biobricking of {Pdps}   
cgttaGGATCCaatctcatatcctcttgatg

pBCa1601AC-K112323

PCR ca998/JH090R on pBjh1601AC-K112321-2    (445 bp, EcoRI/BamHI)
Sub into pBjh1601AC                      (EcoRI/BamHI, 3195+910, L)
Product is pBjh1601AC-K112323
-----------------------------------------------
ca998   Forward biobricking of {H-NS!} Salmonella   
gtatcacgaggcagaatttcag
JH090R   Reverse biobricking of {H-NS!} Salmonella   
cgttaGGATCCTTATTcCTTGATCAGG

pBCa1601CK-K112324

Digest (BgIII methylated) K112902 CA, (BamHI methylated) K112709 AK      (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112324

pBCa1601CK-K112325

Digest (BgIII methylated) K112704 CA, (BamHI methylated) K112709 AK      (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112325

pBCa1601AC-K112327

Digest (BgIII methylated) K112230 AK, (BamHI methylated) K112203 KC      (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112327

pBCa1601AC-K112328

Digest (BgIII methylated) K112227 AK, (BamHI methylated) K112102 KC      (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112328

pBCa1601AC-K112329

Digest  (BgIII methylated) K112230 AK, (BamHI methylated) K112113 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112329

pBCa1601AC-K112330

Digest K112614 AK (BgIII, XhoI)
Digest K112105 KC(BamHI, XhoI)
Product is pBjh1601AC-K112330

pBCa1601AC-K112331

Digest K112615 AK (BgIII, XhoI)
Digest K112108 KC(BamHI, XhoI)
Product is pBjh1601AC-K112331

pBCa1601AC-K112332

Digest  (BgIII methylated) K112615 AK, (BamHI methylated) K112216 KC      (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112332

pBCa1601AC-K112333

Digest (BgIII methylated) K112614 AK, (BamHI methylated) K112222 KC      (BamHI/BgIII/XHoI)
Product is pBjh1601AC-K112333

pBca1601AK-K112334

Digest (BgIII methylated) K112230 AC, (BamHI methylated) K112324 CK     (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112334

pBca1601AK-K112335

Digest (BgIII methylated) K112227 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112335

pBca1601AK-K112336

Digest (BgIII methylated) K112614 AC	  (BamHI/XhoI)
Digest (BamHI methylated) K112324 CK      (BgIII/XHoI)
Product is pBjh1601AK-K112336

pBca1601AK-K112337

Digest (BgIII methylated) K112615 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112337

pBca1601AK-K112339

Digest (BgIII methylated) K112327 AC     (BamHI/XhoI)
Digest (BamHI methylated) K112324 CK     (BgIII/XHoI)
Product is pBjh1601AK-K112339

pBca1601AK-K112340

Digest (BgIII methylated) K112328 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112340

pBca1601AK-K112341

Digest (BgIII methylated) K112329 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112341

pBca1601AK-K112342

Digest (BgIII methylated) K112330 AC, (BamHI methylated) K112324 CK      (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112342

pBca1601AK-K11234

Digest (BgIII methylated) K112332 AC, (BamHI methylated) K112324 CK     (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112344

pBca1601AK-K112348

Digest (BgIII methylated) K112328 AC, (BamHI methylated) K112325 CK     (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112348

pBca1601AK-K112349

Digest (BgIII methylated) K112329 AC, (BamHI methylated) K112325 CK     (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112349

pBca1601AK-K112350

Digest (BgIII methylated) K112330 AC	 (BamHI/XhoI)
Digest (BamHI methylated) K112325 CK     (BgIII/XHoI)
Product is pBjh1601AK-K112350

pBca1601AK-K112351

Digest (BgIII methylated) K112331 AC	 (BamHI/XhoI)
Digest (BamHI methylated) K112325 CK     (BgIII/XHoI)
Product is pBjh1601AK-K112351

pBca1601CK-K112354

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112334 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112354

pBca1601CK-K112355

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112335 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112354

pBca1601CK-K112356

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112336 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112354

pBca1601CK-K112357

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112337 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112354

pBca1601CK-K112360

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112340 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112360

pBca1601CK-K112361

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112341 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112361

pBca1601CK-K112362

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112342 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112362

pBca1601CK-K112363

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112343 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112363

pBca1601CK-K112368

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112348 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112368

pBca1601CK-K112369

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112349 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112369

pBca1601CK-K112370

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112350 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112370

pBca1601CK-K112371

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112351 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112371

pBca1601CK-K112372

Digest (BgIII methylated) K112121 CA, (BamHI methylated) K112709 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112372

pBca1601CK-K112373

Digest (BgIII methylated) BBa_K112232 CA, (BamHI methylated) K112709 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112373

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