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Purdue/10 July 2008
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After letting the transformation solutions sit out overnight, there are enough cells to do bead plating. | After letting the transformation solutions sit out overnight, there are enough cells to do bead plating. | ||
| - | Bead plated, and let incubate at 37C o/n. | + | Bead plated control (pUC19), LacZalpha, and LacY genes, and let incubate at 37C o/n. |
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| + | Found via email from Chris French (Edinburgh) that RFP will make a visible red color. Therefore, we don't need any fancy MacConkey agar combined with a lac operon. Yay! | ||
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| + | Revision 10385: First reporter = phr + RFP, 2nd reporter = SOS (recA) + LacZ. We'll still put X-gal in the substrate. | ||
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| + | Probable RFP gene to use: J06702--the mCherry (modified mRFP-1) gene with an RBS. It's on pSB1A2, 869 bp long, AK resistant, on plate 1004 well 7H. The only problem is that the QC data isn't very good. | ||
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| + | Also: since the stabs from IGEM for the SOS and LacZ genes grew up so well, we're making glycerol stocks. Today, made overnights in amp LB. Put in 37C incubator. | ||
'''Edited by Janie Stine''' | '''Edited by Janie Stine''' | ||
Latest revision as of 19:30, 10 July 2008
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After letting the transformation solutions sit out overnight, there are enough cells to do bead plating. Bead plated control (pUC19), LacZalpha, and LacY genes, and let incubate at 37C o/n.
Found via email from Chris French (Edinburgh) that RFP will make a visible red color. Therefore, we don't need any fancy MacConkey agar combined with a lac operon. Yay!
Revision 10385: First reporter = phr + RFP, 2nd reporter = SOS (recA) + LacZ. We'll still put X-gal in the substrate.
Probable RFP gene to use: J06702--the mCherry (modified mRFP-1) gene with an RBS. It's on pSB1A2, 869 bp long, AK resistant, on plate 1004 well 7H. The only problem is that the QC data isn't very good.
Also: since the stabs from IGEM for the SOS and LacZ genes grew up so well, we're making glycerol stocks. Today, made overnights in amp LB. Put in 37C incubator.
Edited by Janie Stine
