Team:Rice University/Notebook/11 July 2008
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****1uL BSA | ****1uL BSA | ||
****22uL dH2O | ****22uL dH2O | ||
+ | ***DCC | ||
+ | ***Ligation Mix | ||
+ | **** 10uL DCCed DNA | ||
+ | ****1uL T4 | ||
+ | ****2uL Buffer | ||
+ | ****.5uL Vector | ||
+ | ****7.5 dH2O | ||
+ | |||
+ | *Taylor Stevenson | ||
+ | **Prepare 5mL LB O/N of VCS257 (37*C) and VCS257 lambda lysogens (30*C) for preparation of electro-competent cells | ||
+ | |||
+ | <BR><BR><BR><BR> | ||
+ | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center" | ||
+ | !align="center"|[[Team:Rice_University|Home]] | ||
+ | !align="center"|[[Team:Rice_University/Team|The Team]] | ||
+ | !align="center"|[[Team:Rice_University/Project|The Project]] | ||
+ | !align="center"|[[Team:Rice_University/Parts|Parts Submitted to the Registry]] | ||
+ | !align="center"|[[Team:Rice_University/Modeling|Modeling]] | ||
+ | !align="center"|[[Team:Rice_University/Notebook|Notebook]] | ||
+ | |} |
Latest revision as of 18:05, 11 July 2008
- David Ouyang
- Digest and Ligate Putative Amber Suppressors
- Digest Mix
- 1uL EcoRI
- 1uL PstI
- 5uL NEB3
- 20uL Dilute DNA
- 1uL BSA
- 22uL dH2O
- DCC
- Ligation Mix
- 10uL DCCed DNA
- 1uL T4
- 2uL Buffer
- .5uL Vector
- 7.5 dH2O
- Digest Mix
- Digest and Ligate Putative Amber Suppressors
- Taylor Stevenson
- Prepare 5mL LB O/N of VCS257 (37*C) and VCS257 lambda lysogens (30*C) for preparation of electro-competent cells
Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Notebook |
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